miRNA-1284, a regulator of HMGB1, inhibits cell proliferation and migration in osteosarcoma

Abstract

Previous literatures have reported the role of human micro RNA-1284 (hsa-miR-1284, in short miR-1284) in diverse cancers. However, its biological function in osteosarcoma pathogenesis remains unknown. In the present study, we investigated the potential role of miR-1284 in osteosarcoma. Expression of miR-1284 and high mobility group box 1 (HMGB1) were examined in 80 tissues obtained from 40 patients. MiR-1284 level was measured in five osteosarcoma cell lines. Relative luciferase activity and HMGB1 expression were examined in MG-63 and U2OS cells transfected with wild-type or mutant 3′-UTR of HMGB1 in the presence of miR-1284 mimics or miR-NC. Cell viability, colony formation, and cell migration were measured in MG-63, U2OS and hFOB 1.19 cells, which were transfected with miR-1284 mimics or miR-NC. In the rescue experiments, recombinant HMGB1 plasmid was transfected into MG-63 and U2OS cells, and cell viability and migration were determined again. Our results indicated that relative level of miR-1284 was lower in tumor tissues compared with its adjacent tissues and it was found suppressed at lower levels in MG-63 and U2OS cell lines. Expression of HMGB1 is significantly elevated in tumor tissues and negatively correlated with miR-1284 expression. MiR-1284 exerted its function by directly binding to 3′-UTR of HMGB1 and regulates expression of HMGB1. The overexpression of miR-1284 inhibited the cell proliferation and migration, and altered the protein expression of epithelial–mesenchymal transition (EMT)-associated genes (E-cadherin, N-cadherin, Vimentin, and Snail), which was reversed by HMGB1 overexpression. In conclusion, miR-1284 can function as a new regulator to inhibit osteosarcoma cell proliferation and migration by targeting HMGB1.

CD59 receptor targeted delivery of miRNA-1284 and cisplatin-loaded liposomes for effective therapeutic efficacy against cervical cancer cells

“…Earlier reports have revealed that downregulation of HMGB1 could lead to the inhibition of cell proliferation. Results clearly highlight our expectation that downregulation of HMGB1 could be directly related to the proliferation of cervical cancer cells (Wu and Yang 2018;Lv and Guan 2018). It is worth noting that CD59-antibody conjugated formulation exhibited a significantly lower cell viability compared to that of free CDDP.…”

MicroRNA‐320a inhibits invasion and metastasis in osteosarcoma by targeting cytoplasmic polyadenylation element‐binding protein 1

“…For example, miRNA‐21 was found upregulated in osteosarcoma tissues and cells and inhibition of miRNA‐21 could inhibit osteosarcoma cell proliferation . miR‐1284 was proved to be a new regulator to inhibit osteosarcoma cell proliferation and migration …”

Rs145204276 and rs4759314 affect the prognosis of prostate cancer by modulating the GAS5/miR-1284/HMGB1 and HOTAIR/miR-22/HMGB1 signalling pathways

“…HMGB1 has been implicated in the pathogenesis and prognosis of PC [19,20]. Meanwhile, it was reported that both miR-1284 and miR-22 function as regulators of HMGB1 expression [21,22]. In addition, GAS5 and HOTAIR play a role as competing endogenous RNAs against miR-1284 and miR-22, respectively [21,23].…”