miRNA-185 regulates the VEGFA signaling pathway in dairy cows with retained fetal membranes

Zheng, Chao; Zou, X.; Lin, Hsin-Hung; Zhao, Bingjie; Zhang, M.L.; Luo, Chen; Fu, Shixin

doi:10.1016/j.theriogenology.2017.12.050

Cited by 9 publications

(11 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MiRNAs encode 1%–5% of all mammalian genes and regulate the expression of approximately 30% of all protein‐coding genes. In a previous study, miRNA‐185 was differentially expressed in the maternal placenta of Holstein cows with RP (Zheng et al., 2018a,b). Importantly, phospholipase A (PLA) was identified as a key limiting enzyme in the production of arachidonic acid (ARA), which is regulated by the VEGFA signalling pathway (Roskoski Jr, 2012).…”

Section: The Molecular Role In the Retention Of The Placentamentioning

confidence: 87%

“…Therefore, Zheng et al. (2018a,b) aimed to explore the pathogenesis of RP by investigating the regulatory relationship between miRNA‐185 and the VEGFA signalling pathway. Quantitative polymerase chain reaction (qPCR) and western blotting (WB) were performed to detect the mRNA and proteins levels, respectively, of genes involved in the VEGFA signalling pathway.…”

Section: The Molecular Role In the Retention Of The Placentamentioning

confidence: 99%

“…Importantly, it was found that STIM1 was a target gene of miRNA‐185, and regulated by miRNA‐in UCE cells. It has been reported that miRNA‐185 can influence the release of the foetal placenta via the VEGFA signalling pathway (Zheng et al., 2018a,b). VEGFA, as a G protein, can bind the VEGFA receptor to activate PLC downstream.…”

Section: The Molecular Role In the Retention Of The Placentamentioning

confidence: 99%

See 2 more Smart Citations

Latest update on predictive indicators, risk factors and ‘Omic’ technologies research of retained placenta in dairy cattle – A review

Amin

Hussein

2022

Reprod Domestic Animals

View full text Add to dashboard Cite

Retained placenta is a pathological condition defined as failure to expel foetal membranes within 12–24 h after parturition. It instigates important financial losses to the dairy industry causing considerable economic loss. It is a multifactorial disorder. Although different hypotheses have been proposed, contradictory results and no single factor explains the development of the disease. This indicates that there are overall significant gaps in our knowledge about the various factors affecting the aetiopathology and pathobiology of the retained placenta. Thus, it will be of great interest to investigate the potential contributing factors that influence the occurrence of RP which provide us with the mechanisms to avoid the occurrence of the disease. In addition, different predictive indicators of RP should be a part of our research, thus providing the essential procedures applied to deal with the disease and the development of novel therapeutic and prophylactic strategies in its management. Furthermore, lately, the molecular role takes attention to understanding its effect in the occurrence of the disease. Such information might help dairy scientists in the future to design better management and preventive practices for RP in dairy cows.

show abstract

Section: The Molecular Role In the Retention Of The Placentamentioning

confidence: 87%

Section: The Molecular Role In the Retention Of The Placentamentioning

confidence: 99%

Section: The Molecular Role In the Retention Of The Placentamentioning

confidence: 99%

See 1 more Smart Citation

Latest update on predictive indicators, risk factors and ‘Omic’ technologies research of retained placenta in dairy cattle – A review

Amin

Hussein

2022

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…Vascular endothelial growth factor (VEGF) A, reportedly regulated by miRNA-185, may induce the release of arachidonic acid through the signaling pathway that influences RFM (10,33). With miRNA-185 incriminated by the activation of the VEGFA signaling pathway, the expression levels of most of the investigated genes (VEGFA, PLC, PRK, RAF, MEK, MAPK, and PLA) are reduced particularly by the abnormal expression of P-p44/42 MAPK, which influenced the release of fetal placenta after calving (103). The overexpression of miRNA-185 inhibited the VEGFA signaling pathway, which plays a role in retaining the fetal membrane during postpartum that may lead to endometritis.…”

Section: Mirna-185 Regulates the Vegfa Signaling Pathwaymentioning

confidence: 99%

MicroRNAome: Potential and Veritable Immunomolecular Therapeutic and Diagnostic Baseline for Lingering Bovine Endometritis

Oladejo

et al. 2020

Front. Vet. Sci.

View full text Add to dashboard Cite

The bovine endometrium is a natural pathogen invasion barrier of the uterine tissues' endometrial epithelial cells that can resist foreign pathogen invasion by controlling the inflammatory immune response. Some pathogens suppress the innate immune system of the endometrium, leading to prolonged systemic inflammatory response through the blood circulation or cellular degradation resulting in bovine endometritis by bacterial endotoxins. The microRNA (miRNA) typically involves gene expression in multicellular organisms in post-transcription regulation by affecting both the stability and the translation of messenger RNA. Accumulated evidence suggests that miRNAs are important regulators of genes in several cellular processes. They are a class of endogenous non-coding RNAs, which play pivotal roles in the inflammatory response of reproductive diseases. Studies confirmed that miRNAs play a key regulatory role in various inflammatory diseases by mediating the molecular mechanism of inflammatory cytokines via signal pathways. It implicates some miRNAs in the occurrence of bovine endometritis, resorting to regulating the activities of some inflammatory cytokines, chemokine, differentially expressed genes, and protein through modulating of specific cellular signal pathways functions. This review dwells on improving the knowledge of the role of miRNAs involvement in inflammatory response as to early diagnosis, control, and prevention of bovine endometritis and consequently enlighten on the molecular improvement of the genes coded by various differentially expressed miRNA through the need to adopt recent genetic technologies and the development of new pharmaceutical preparations.

show abstract

“…Recent studies showed that various miRNAs play major roles in the molecular mechanism of placental development ( 5 – 7 ). In addition, miRNAs are involved in the biological functions of trophoblast and endometrial epithelial cells ( 8 – 10 ). For example, Zheng et al found that miRNA-185 regulates endometrial epithelial cells by targeting stromal interaction molecule 1 (STIM1) ( 9 ) and that miRNA-185 mediates the vascular endothelial growth factor (VEGF) A signaling pathway to regulate RP ( 10 ).…”

Section: Introductionmentioning

confidence: 99%

miRNA-150_R-1 mediates the HIF-1/ErbB signaling pathway to regulate the adhesion of endometrial epithelial cells in cows experiencing retained placenta

Li²,

Wang³

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

Retained placenta (RP) refers to reproductive disorders caused by the failure of fetal membranes to be expelled 12 h after delivery in dairy cows. Postpartum adhesion of the fetal membranes to the uterus causes diseases such as mastitis or endometritis, which threatening the profitability of the dairy industry. Emerging evidence suggests that micro RNAs (miRNAs) play crucial roles in various processes, such as the occurrence and progression of fetal membranes discharge. However, the molecular mechanisms of miRNAs in RP remain unknown. In this study, we performed RNA-sequencing to characterize the expression profiles of mRNAs and miRNAs in caudal vein blood samples of postpartum Holstein cows whose fetal membranes were discharged normally or retained to identify RP-related genes and evaluate their molecular mechanisms. We identified 44 differentially expressed miRNAs (19 upregulated and 25 downregulated) and 706 differentially expressed mRNAs (325 upregulated and 381 downregulated) in the RP group compared to the normal fetal membranes discharge group. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis revealed that differentially expressed mRNAs were mainly enriched in the extracellular matrix, cell adhesion, and autoimmunity-related biological processes or pathways. Further analyses using RNA-sequencing, a dual luciferase reporter system, quantitative reverse transcription-PCR, immunofluorescence, and western blotting verified that endothelial PAS domain protein 1 (EPAS1) is regulated by miR-150_R-1 in endometrial epithelial cells. We demonstrated the relationship between EPAS1 and RP and confirmed that EPAS1 is upregulated in the blood and placenta of cows that experience RP. Further, we proposed a model of the miRNA-mRNA negative regulatory network mediated by the HIF-1/ErbB signaling pathway to show its regulatory role in RP.

show abstract

miRNA-185 regulates the VEGFA signaling pathway in dairy cows with retained fetal membranes

Cited by 9 publications

References 29 publications

Latest update on predictive indicators, risk factors and ‘Omic’ technologies research of retained placenta in dairy cattle – A review

Latest update on predictive indicators, risk factors and ‘Omic’ technologies research of retained placenta in dairy cattle – A review

MicroRNAome: Potential and Veritable Immunomolecular Therapeutic and Diagnostic Baseline for Lingering Bovine Endometritis

miRNA-150_R-1 mediates the HIF-1/ErbB signaling pathway to regulate the adhesion of endometrial epithelial cells in cows experiencing retained placenta

Contact Info

Product

Resources

About