MiRNA-199a-3p Regulates C2C12 Myoblast Differentiation through IGF-1/AKT/mTOR Signal Pathway

Long, Jia; Li, Yuefeng; Wu, Guofang; Song, Ziyi; Lu, Hongzhao; Song, Chengchuang; Zhang, Qiangling; Zhu, Jiayu; Yang, Gongshe; Shi, Xin’e

doi:10.3390/ijms15010296

Cited by 80 publications

(64 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2A). These data were similar to the fluctuation observed with miR-199a-3p, which was shown to inhibit C2C12 myoblast differentiation (43). Therefore, we speculated that the expression of miR-351-5p in formed myotubes would negatively affect C2C12 myoblast differentiation.…”

Section: Mir-351-5p Negatively Modulates Myoblast Differentiationsupporting

confidence: 87%

MicroRNA‐351‐5p mediates skeletal myogenesis by directly targeting lactamase‐β and is regulated bylnc‐mg

Zhang

Zhao

et al. 2018

FASEB j.

View full text Add to dashboard Cite

Skeletal muscle is an important and complex organ with a variety of functions in humans and animals. Skeletal myogenesis is a multistep and complex process, and increasing evidence suggests that microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) play critical roles in skeletal myogenesis. In this study the expression of miR-351-5p is dynamically regulated during skeletal myogenesis in vitro and in vivo. Cell-counting kit-8, qRT-PCR, and EdU immunofluorescence analysis showed that miR-351-5p overexpression promoted the proliferation and inhibited the differentiation of C2C12 myoblast, whereas inhibition of miR-351-5p had the opposite effect. In addition, miR-351-5p mediated the regulation of muscle fiber type transition in vivo. In vitro, loss of miR-351-5p in muscle tissues promoted muscle hypertrophy and increased slow-twitch fibers in the gastrocnemius muscles of mice. Luciferase reporter assay and functional analyses demonstrated that lactamase β ( LACTB) is a direct target of miR-351-5p involved in the regulation of skeletal myogenesis. Expression levels of a myogenesis-associated lncRNA ( lnc-mg) correlated negatively with miR-351-5p and positively with LACTB during C2C12 myoblast proliferation and differentiation. Further analyses showed that lnc-mg acted as a molecular sponge for miR-351-5p, demonstrating its involvement in the negative regulation of LACTB by miR-351-5p during skeletal myogenesis. These findings indicate that miRNA-351-5p functions in skeletal myogenesis by targeting LACTB and is regulated by lnc-mg, supporting the role of the competing endogenous RNA network in skeletal myogenesis.-Du, J., Zhang, P., Zhao, X., He, J., Xu, Y., Zou, Q., Luo, J., Shen, L., Gu, H., Tang, Q., Li, M., Jiang, Y., Tang, G., Bai, L., Li, X., Wang, J., Zhang, S., Zhu, L. MicroRNA-351-5p mediates skeletal myogenesis by directly targeting lactamase β and is regulated by lnc-mg.

show abstract

Section: Mir-351-5p Negatively Modulates Myoblast Differentiationsupporting

confidence: 87%

MicroRNA‐351‐5p mediates skeletal myogenesis by directly targeting lactamase‐β and is regulated bylnc‐mg

Zhang

Zhao

et al. 2018

FASEB j.

View full text Add to dashboard Cite

show abstract

“…Among microRNAs carried by EVs, miR-223 represents a novel regulator of macrophage polarization, being responsible of suppressing classic pro-inflammatory pathways and enhancing the alternative anti-inflammatory responses, whereas the enforced expression of mir-146b in human monocytes leads to a significant reduction in the production of several proinflammatory cytokines and chemokines, such as IL6 [25]. In addition, the increased expression pattern of miR-126 and miR-199a plays important roles in the repair process restoring vascular integrity and inducing cell differentiation, respectively [27,28]. An increasing number of literature reports indicate that MSCs possess the capacity to reduce inflammation and to promote tissue repair processes by their paracrine activity [13,46].…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal Stem Cell-Derived Extracellular Vesicles as Mediators of Anti-Inflammatory Effects: Endorsement of Macrophage Polarization

Sicco

Reverberi

Balbi

et al. 2017

Stem Cells Translational Medicine

459

359

View full text Add to dashboard Cite

Mesenchymal Stem Cells (MSCs) are effective therapeutic agents enhancing the repair of injured tissues mostly through their paracrine activity. Increasing evidences show that besides the secretion of soluble molecules, the release of extracellular vesicles (EVs) represents an alternative mechanism adopted by MSCs. Since macrophages are essential contributors toward the resolution of inflammation, which has emerged as a finely orchestrated process, the aim of the present study was to carry out a detailed characterization of EVs released by human adipose derived‐MSCs to investigate their involvement as modulators of MSC anti‐inflammatory effects inducing macrophage polarization. The EV‐isolation method was based on repeated ultracentrifugations of the medium conditioned by MSC exposed to normoxic or hypoxic conditions (EVNormo and EVHypo). Both types of EVs were efficiently internalized by responding bone marrow‐derived macrophages, eliciting their switch from a M1 to a M2 phenotype. In vivo, following cardiotoxin‐induced skeletal muscle damage, EVNormo and EVHypo interacted with macrophages recruited during the initial inflammatory response. In injured and EV‐treated muscles, a downregulation of IL6 and the early marker of innate and classical activation Nos2 were concurrent to a significant upregulation of Arg1 and Ym1, late markers of alternative activation, as well as an increased percentage of infiltrating CD206pos cells. These effects, accompanied by an accelerated expression of the myogenic markers Pax7, MyoD, and eMyhc, were even greater following EVHypo administration. Collectively, these data indicate that MSC‐EVs possess effective anti‐inflammatory properties, making them potential therapeutic agents more handy and safe than MSCs. stem cells translational medicine 2017 Stem Cells Translational Medicine 2017;6:1018–1028

show abstract

“…Inappropriate over-expression of miR-486 resulted in abnormal regeneration, presumably through the misregulation of many target genes (116). Another miRNA, miR-199a-3p, was recently shown to regulate multiple targets within the AKT/mTOR signaling pathway in vitro; however, the importance of miR-199a-3p in the regulation of skeletal muscle regeneration remains unknown (117). These findings indicate that components of the AKT/mTOR signaling pathway are subject to regulation by both muscle-specific and non-muscle-specific miRNAs.…”

Section: Manipulation Of Microrna Expression On De Novo Skeletal Mmentioning

confidence: 99%

The role of microRNAs in skeletal muscle health and disease

2015

View full text Add to dashboard Cite

Over the last decade non-coding RNAs have emerged as importance regulators of gene expression. In particular, microRNAs are a class of small RNAs of ~ 22 nucleotides that repress gene expression through a post-transcriptional mechanism. MicroRNAs have been shown to be involved in a broader range of biological processes, both physiological and pathological, including myogenesis, adaptation to exercise and various myopathies. The purpose of this review is to provide a comprehensive summary of what is currently known about the role of microRNAs in skeletal muscle health and disease.

show abstract

MiRNA-199a-3p Regulates C2C12 Myoblast Differentiation through IGF-1/AKT/mTOR Signal Pathway

Cited by 80 publications

References 29 publications

MicroRNA‐351‐5p mediates skeletal myogenesis by directly targeting lactamase‐β and is regulated bylnc‐mg

MicroRNA‐351‐5p mediates skeletal myogenesis by directly targeting lactamase‐β and is regulated bylnc‐mg

Mesenchymal Stem Cell-Derived Extracellular Vesicles as Mediators of Anti-Inflammatory Effects: Endorsement of Macrophage Polarization

The role of microRNAs in skeletal muscle health and disease

Contact Info

Product

Resources

About