miRNA-218/FANCI is associated with metastasis and poor prognosis in lung adenocarcinoma: a bioinformatics analysis

Ye, Guanchao; Liu, Yafei; Huang, Lan; Zhang, Chunyang; Sheng, Yinliang; Wu, Bin; Wu, Chunli; Qi, Yu

doi:10.21037/atm-21-3823

Cited by 10 publications

(4 citation statements)

References 27 publications

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“…16-HBE cell line was obtained from the Basic Department of Chengde Medical College (Chengde, China). The cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (Vivacell, Shanghai, China) and 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA) in a humidified 37 °C incubator with 5% CO 2 [ 13 ]. The cells (approximately 4 × 10 5 /well) were plated in six-well plates and incubated for 24 h [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

Role of miR-584-5p in Lipopolysaccharide-Stimulated Human Bronchial Epithelial Cell Inflammation and Apoptosis

Zhang

Lin-ying

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Acute lung injury (ALI)/acute respiratory distress syndrome is a common clinical syndrome characterized by respiratory failure. MicroRNAs (miRNAs) are closely related to ALI and acute respiratory distress syndrome. TargetScan software analysis showed that miR-584-5p can bind to the 3ʹ noncoding region of TLR4, which is involved in the occurrence and development of ALI, thereby affecting the inflammatory pathway and inflammation development. Thus, we aimed to determine whether miR-584-5p affects ALI. Human bronchial epithelial (16-HBE) cells were transfected with miR-584-5p mimics or inhibitors and then stimulated with lipopolysaccharide (LPS).The cell viability, apoptosis, release of proinflammatory factors, mTOR, and NF-κB pathway protein expression were evaluated respectively. Mimic584 increased, whereas inhibitor584 decreased, LPS-stimulated inflammation. The protein expression of inflammatory factors was significantly increased in 16-HBE cells in the mimic584 + LPS group and decreased in the inhibitor584 + LPS group. Mimic584 activated mTOR and the NF-κB-related proteins P65 and p-p65, whereas inhibitor584 inactivated the proteins in 16-HBE cells. Overexpression of miR-584 significantly promoted apoptosis in LPS-stimulated 16-HBE cells. There were no differences in the proliferation and cell cycle of LPS-stimulated 16-HBE cells regardless of mimic584 or inhibitor584 transfection. Collectively, we demonstrated that inhibitor584 can alleviate ALI-induced expression of inflammatory factors via mTOR signaling and the NF-κB pathway. In conclusion, we found that inhibitor584 transfection could be a potential therapeutic strategy for ALI.

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Section: Methodsmentioning

confidence: 99%

Role of miR-584-5p in Lipopolysaccharide-Stimulated Human Bronchial Epithelial Cell Inflammation and Apoptosis

Zhang

Lin-ying

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…Which may contribute to cancer cells' survival, and promote tumor cell resistance against radiotherapy and chemotherapy [10]. Besides, the functions of the FANCI protein have been investigated in various cancers, and FANCI has also been proven to be regarded as a prognostic biomarker, given its high expression is associated with poor survival rate and therapeutic outcome in lung adenocarcinoma, cervical cancer, and ovarian cancer [11][12][13]. However, there is currently a lack of studies on the relationship between the expression of FANCI and liver cancer.…”

Section: Ivyspringmentioning

confidence: 99%

FANCI is Associated with Poor Prognosis and Immune Infiltration in Liver Hepatocellular Carcinoma

Hou¹,

Li²,

Yu³

et al. 2023

Int. J. Med. Sci.

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Objective: This study aimed to validate FANCI as a potential marker for both prognosis and therapy in liver hepatocellular carcinoma. Method: FANCI expression data were acquired from GEPIA, HPA, TCGA, and GEO databases. The impact of clinicopathological features was analyzed by UALCAN. The prognosis of Liver Hepatocellular Carcinoma (LIHC) patients with highly expressed FANCI was constructed utilizing Kaplan-Meier Plotter. GEO2R was employed to identify differentially expressed genes (DEGs). Metascape was used to analyze functional pathways correlations. Protein-Protein interaction (PPI) networks were generated by Cytoscape. Furthermore, molecular complex detection (MCODE) was utilized to recognize Hub genes, which were selected to establish a prognostic model. Lastly, the relationship between FANCI and immune cell infiltration in LIHC was examined. Results: Compared to adjacent tissues, FANCI expression levels were significantly higher in LIHC tissues and were positively correlated to the cancer grade, stage, and prior hepatitis B virus (HBV) infection. High expression of FANCI was found to be associated with poor prognosis in LIHC (HR=1.89, p<0.001). DEGs that were positively correlated with FANCI were involved in various processes, including the cell cycle, VEGF pathway, immune system processes, and biogenesis of ribonucleoproteins. MCM10, TPX2, PRC1, and KIF11 were identified as key genes closely related to FANCI and poor prognosis. A reliable five-variable prognostic model was constructed with strong predictive capability. Lastly, a positive correlation was observed between FANCI expression and tumor-infiltration levels of CD8+ T cells, B cells, regulatory T (Tregs), CD4+ T helper 2 (Th2), and macrophage M2 cells. Conclusion: FANCI may hold promise as a potential biomarker for predicting prognostic outcomes, and a valuable therapeutic target for LIHC patients, with a focus on anti-proliferation, anti-chemoresistance, and combination with immunotherapy.

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“…FANCI is a potential biomarker for the prognosis of hepatocellular carcinoma [18]. A lot of relevant papers have also shown that the high expression of FANCI in tumors is closely related to the survival rate and poor prognosis of cancer patients, such as FANCI may interact with extracellular matrix receptors to affect the poor prognosis of lung adenocarcinoma [19] and pathogenic FANCI mutations increase the risk of familial ovarian cancer [20]. However, the reports of the mechanism of FANCI in glioma development and progression are still very few.…”

Section: Introductionmentioning

confidence: 99%

Knocking down of FANCI expression inhibits the biological behavior of glioma and mediates apoptosis by downregulating the Akt/Bcl2 pathway

Li,

Wang,

Liu

et al. 2024

Preprint

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Purpose Previous studies have shown that FANCI has cancer susceptibility, and high expression of FANCI promotes the progression of breast cancer, ovarian cancer, and other cancers. However, the potential mechanism of action of FANCI in glioma progression is unclear. Methods To explore the role of FANCI in glioma progression, we determined the expression of FANCI in glioma patients and its relationship with prognosis through database analysis and gene chip. And then further conducted in vitro functional experiments (overexpression and knockdown) and in vivo nude mouse xenograft model experiments. Results This study found that FANCI was significantly overexpressed in glioma, positively correlated with WHO grade, and closely related to patient prognosis. In vitro functional experiments showed that inhibiting the expression of FANCI could inhibit the proliferation, migration, and invasion of glioma and promote apoptosis. At the same time, the nude mouse xenograft model also confirmed that inhibition of FANCI could inhibit glioma in vivo. In addition, the low expression of FANCI inhibited the phosphorylation of Akt and the expression of Bcl-2 by western blotting. Conclusion FANCI promotes glioma growth and may mediate apoptosis by regulating Akt/Bcl-2. This study preliminarily explored the role of FANCI in glioma growth and provided data support for further application of FANCI in clinical practice.

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miRNA-218/FANCI is associated with metastasis and poor prognosis in lung adenocarcinoma: a bioinformatics analysis

Cited by 10 publications

References 27 publications

Role of miR-584-5p in Lipopolysaccharide-Stimulated Human Bronchial Epithelial Cell Inflammation and Apoptosis

Role of miR-584-5p in Lipopolysaccharide-Stimulated Human Bronchial Epithelial Cell Inflammation and Apoptosis

FANCI is Associated with Poor Prognosis and Immune Infiltration in Liver Hepatocellular Carcinoma

Knocking down of FANCI expression inhibits the biological behavior of glioma and mediates apoptosis by downregulating the Akt/Bcl2 pathway

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