miRNA-222-3p enhances the proliferation and suppresses the apoptosis of acute myeloid leukemia cells by targeting Axin2 and modulating the Wnt/β-catenin pathway

Liu, Zhenyan; Zhong, Liang; Dan, Wenran; Chu, Xuan; Liu, Chen; Luo, Xu; Zhang, Zhonghui; Yang, Lu; Wan, Peng; Wang, Xiao; Liu, Beizhong

doi:10.1016/j.bbrc.2022.06.054

Cited by 3 publications

(3 citation statements)

References 28 publications

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“…MiR-222 was highly downregulated but was not associated with the response to treatment or FAB classification [133]. On the contrary, other studies suggest that miR-222 is overexpressed in AML and upregulates the Wnt/β-catenin pathway by inhibiting Axin2, a tumor suppressor [134]. Also, miR-222 and miR-181 had a higher expression in AML than in MDS both in peripheral blood and bone marrow [135].…”

Section: Micrornas Associated With a Bad Prognosis In Aml And M2 Pola...mentioning

confidence: 89%

MicroRNAs Associated with a Bad Prognosis in Acute Myeloid Leukemia and Their Impact on Macrophage Polarization

Jimbu,

Mesaros,

Joldes

et al. 2024

Biomedicines

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MicroRNAs (miRNAs) are short, non-coding ribonucleic acids (RNAs) associated with gene expression regulation. Since the discovery of the first miRNA in 1993, thousands of miRNAs have been studied and they have been associated not only with physiological processes, but also with various diseases such as cancer and inflammatory conditions. MiRNAs have proven to be not only significant biomarkers but also an interesting therapeutic target in various diseases, including cancer. In acute myeloid leukemia (AML), miRNAs have been regarded as a welcome addition to the limited therapeutic armamentarium, and there is a vast amount of data on miRNAs and their dysregulation. Macrophages are innate immune cells, present in various tissues involved in both tissue repair and phagocytosis. Based on their polarization, macrophages can be classified into two groups: M1 macrophages with pro-inflammatory functions and M2 macrophages with an anti-inflammatory action. In cancer, M2 macrophages are associated with tumor evasion, metastasis, and a poor outcome. Several miRNAs have been associated with a poor prognosis in AML and with either the M1 or M2 macrophage phenotype. In the present paper, we review miRNAs with a reported negative prognostic significance in cancer with a focus on AML and analyze their potential impact on macrophage polarization.

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Section: Micrornas Associated With a Bad Prognosis In Aml And M2 Pola...mentioning

confidence: 89%

MicroRNAs Associated with a Bad Prognosis in Acute Myeloid Leukemia and Their Impact on Macrophage Polarization

Jimbu,

Mesaros,

Joldes

et al. 2024

Biomedicines

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show abstract

“…The copyright holder for this preprint this version posted June 17, 2024. ; https://doi.org /10.1101/2024.06.13.598949 doi: bioRxiv preprint noncoding RNA or transcriptional regulatory proteins; for example, microRNAs (miRNAs) usually negatively modulate the expression of their target genes, or otherwise regulate important biological processes, such as cell differentiation and apoptosis (29)(30)(31).…”

Section: Introductionmentioning

confidence: 99%

“…Numerous studies have shown that oncogenes are often regulated by upstream noncoding RNA or transcriptional regulatory proteins; for example, microRNAs (miRNAs) usually negatively modulate the expression of their target genes, or otherwise regulate important biological processes, such as cell differentiation and apoptosis (29-31). We hypothesized that a certain miRNA may be involved in regulating the expression of WT1.…”

Section: Introductionmentioning

confidence: 99%

The up-regulation of TGF-β1 by miRNA-132-3p/WT1 is involved in inducing leukemia cells to differentiate into macrophages

Wang,

Zhang

et al. 2024

Preprint

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Although it has been shown that abnormal expression of Wilm’s tumor gene 1 (WT1) is associated with the occurrence of leukemia, the specific mechanism via which it induces leukemia cells to differentiate into macrophages remains poorly understood. Based on the prediction that the microRNA miRNA-132-3p is the miRNA that possibly lies upstream of the WT1 gene, we hypothesized that miRNA-132-3p may participate in the polarization process of macrophages through regulating expression of the WT1 gene. The focus of the present study was therefore to investigate the role of the miRNA-132-3p/WT1 signaling axis in the differentiation of THP-1 leukemia cells into macrophages induced by PMA. The results obtained indicated that, compared with the control group, the proliferation of THP-1 cells was clearly inhibited by PMA, and the cell cycle was arrested at G0/G1 phase, associated with an upregulation of CD11b and CD14 expression. Induced by PMA, the expression level of miRNA-132-3p was increased, WT1 expression was decreased, and the expression level of TGF-β1 was increased. Following transfection with miRNA-132-3p mimics, however, the expression of WT1 in the THP-1 cells was downregulated, with upregulation of the CD11b and CD14 antigens, whereas this downregulation of WT1 mediated by miRNA-132-3p mimics could be reversed by co-transfection with WT1 vector, which was accompanied by downregulation of the CD11b and CD14 antigens. The luciferase activity of the co-transfected miRNA-132-3p mimic + WT1-wild-type (WT) group was found to be statistically significantly lower compared with that of the co-transfected miRNA-132-3p mimic + WT1-mutated (MUT) group. Furthermore, chromatin immunoprecipitation experiments showed that WT1 was able to directly target the promoter of the downstream target gene TGF-β1, which led to the negative modulation of TGF-β1 expression, whereas downregulation of WT1 led to an upregulation of the expression of TGF-β1, which thereby promoted the differentiation of THP-1 cells into macrophages. Taken together, the present study has provided evidence, to the best of the authors’ knowledge for the first time, that the miRNA-132-3p/WT1/TGF-β1 axis is able to regulate the committed differentiation of leukemia cells into macrophages.

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Regulatory mechanism of miR-722 on C5aR1 and its functions against bacterial inflammation in half-smooth tongue sole (Cynoglossus semilaevis)

Liu,

Tan,

Chen

et al. 2023

International Journal of Biological Macromolecules

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miRNA-222-3p enhances the proliferation and suppresses the apoptosis of acute myeloid leukemia cells by targeting Axin2 and modulating the Wnt/β-catenin pathway

Cited by 3 publications

References 28 publications

MicroRNAs Associated with a Bad Prognosis in Acute Myeloid Leukemia and Their Impact on Macrophage Polarization

MicroRNAs Associated with a Bad Prognosis in Acute Myeloid Leukemia and Their Impact on Macrophage Polarization

The up-regulation of TGF-β1 by miRNA-132-3p/WT1 is involved in inducing leukemia cells to differentiate into macrophages

Regulatory mechanism of miR-722 on C5aR1 and its functions against bacterial inflammation in half-smooth tongue sole (Cynoglossus semilaevis)

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