miRNA‑27a promotes the proliferation and inhibits apoptosis of human pancreatic cancer cells by Wnt/β-catenin pathway

Cui, Zhigang; Li, Geng; Kong, Dan

doi:10.3892/or.2017.6120

Cited by 13 publications

(15 citation statements)

References 16 publications

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“…It is well known that miRNA is essential for regulating gene expression, 7 and studies have shown that miRNA can serve as an active role in the promotion or suppression of numerous cancers 8 . Simultaneously, miRNA has also been reported to be involved in controlling biological processes, including cell growth, apoptosis, 9 and cell cycle 10 . For instance, miR‐137 was downregulated in OSCC and related to tumor differentiation, 11 and upregulation of miR‐101 significantly suppressed the proliferation, apoptosis resistance, migration, and invasion of OSCC cells 12 .…”

Section: Introductionmentioning

confidence: 99%

miR‐454 performs tumor‐promoting effects in oral squamous cell carcinoma via reducing NR3C2

Guo

Wang

et al. 2020

J Oral Pathology Medicine

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Background Aberrant miRNAs expression regulates the occurrence and progression of a variety of cancers, including oral squamous cell carcinoma (OSCC). This study aims to illustrate the potential effects of miR‐454/nuclear receptor subfamily 3 group C member 2 (NR3C2) on the biological behaviors of OSCC cells. Methods GEO database was applied to detect and analyze the expression of miR‐545 and NR3C2 in OSCC tissues. Two OSCC cell lines including CAL27 and Tca‐83 were utilized to determine the function of miR‐454/NR3C2 on OSCC cells biological behaviors. miR‐454 and NR3C2 expressions were regulated by miR‐454 mimic/inhibitor and pcDNA3.1‐NR3C2/si‐NR3C2, respectively. Cells biological behaviors were evaluated by cell proliferation, colony formation, and transwell assays. Results The data collected from GEO database indicated that miR‐454 expression was upregulated in OSCC tissues; however, the expression of NR3C2 assumed a downward trend. In vitro experiments, the expression trend of miR‐454 in OSCC cell lines was consistent with that of the trend in tissues, and the OSCC cells growth and movement abilities significantly decreased after miR‐454 depletion. Through co‐transfection experiments, we explored that the abilities of OSCC cell proliferation, colony formation, invasion, and migration obviously reduced after miR‐454 depletion, but these phenomena were mitigated to some extent after NR3C2 silencing. Conclusion The study illustrates that miR‐454 acts as an active regulator to facilitate OSCC cells growth, colony formation, invasion, and migration by targeting NR3C2, which may afford a novel perspective and possibility for the targeted treatment of OSCC.

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Section: Introductionmentioning

confidence: 99%

miR‐454 performs tumor‐promoting effects in oral squamous cell carcinoma via reducing NR3C2

Guo

Wang

et al. 2020

J Oral Pathology Medicine

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“…MicroRNAs (miRNAs) are a species of the non-protein coding RNA family, represented by short, single-stranded RNA approximately 18–22 nucleotides in length, and are the key regulators of post-transcriptional gene expression [8, 9]. Importantly, studies suggested a high association between abnormal miRNA expressions and the development and progression of numerous cancer types [9–12].…”

Section: Introductionmentioning

confidence: 99%

Upregulation of microRNA-23b-3p induced by farnesoid X receptor regulates the proliferation and apoptosis of osteosarcoma cells

Xing

Tao

2019

J Orthop Surg Res

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Background: The downstream targets of farnesoid X receptor (FXR) such as miRNAs have a potent effect on the progression of many types of cancer. We aim to study the effects of FXR on osteosarcoma (OS) development and the potential role of microRNA-23b-3p. Methods: The expressions of FXR and miR-23b-3p in normal osteoblasts and five osteosarcoma cell lines were measured. Their correlations were analyzed by Pearson's test and verified by the introduction of FXR agonist, GW4064. TargetScan predicted that cyclin G1 (CCNG1) was a target for miR-23b-3p. The transfection of FXR siRNA was performed to confirm the correlation between FXR and miR-23b-3p. We further transfected miR-23b-3p inhibitor into MG-63 cells, and the transfected cells were treated with 5 μM GW4064 for 48 h. Quantitative PCR (qPCR) and Western blot were performed for expression analysis. Cell proliferation, cell apoptosis rate, and cell cycle distribution were assessed by clone formation assay and flow cytometry. Results: Scatter plot showed a positive correlation between FXR and miR-23b-3p (Pearson's coefficient test R 2 = 1.00, P = 0.0028). As CCNG1 is a target for miR-23b-3p, the treatment of GW4064 induced the downregulation of CCNG1 through upregulating miR-23b-3p. The inhibition of miR-23b-3p obviously promoted cell viability, proliferation, and cell cycle progression but reduced apoptosis rate of MG-63 cells; however, the treatment of GW4064 could partially reverse the effects of the inhibition of miR-23b-3p on OS cells. Conclusions: Upregulated FXR by GW4064 can obviously suppress OS cell development, and the suppressive effects may rely on miR-23b-3p/CCNG1 pathway.

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“…In 2018, a study was performed on the miRNA27a and its association with pancreatitis cancer (27). In this study, While miRNA27a mimic was applied to overexpress the miRNA27a, an anti miR27a was applied to lower express the same (27). The results demonstrated that the overexpression of hsa-miR-27a-3p leads to cell growth and apoptosis prevention, while the lower expression of it leads to cell growth prevention, leading the cell to apoptosis (27).…”

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Hsa-miR-27a-3p overexpression in men with nonobstructive azoospermia: A case-control study

Norioun

Motovali-Bashi

Javadirad

2020

IJRM

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Background: The role of KDM3A and its downstream genes in male fertility has been approved in animal models. Additionally, the expression shrinkage of KDM3A is significantly correlated with human azoospermia phenotype. Aberrant expression of micro-RNAs could mislead spermatogenesis and mostly lead to diverse phenotypes of male infertility. Objective: The aim of this study was to evaluate the expression level of hsa-miR-27a- 3p in azoospermic men to reveal its possible association with infertility. Materials and Methods: This case-control study was conducted on 30 azoospermic men, of whom, 19 had non obstructive azoospermia (NOA) and 11 obstructive azoospermia (OA) according to the pathological examinations. Comprehensive bioinformatics investigations were performed securely and hsa-miR-27a-3p was selected afterward. Reverse Transcriptase-quantitative polymerase chain reaction (RTqPCR) method was used and statistical analysis was performed to compare the expression level of hsa-miR-27a-3p in both OA and NOA individuals. Results: In silico analysis suggested hsa-miR-27a-3p, with its potential binding ability to target KDM3A transcripts. The expression analysis of candidate hsa-miR-27a-3p indicated its significant overexpression in NOA men. Conclusion: The hsa-miR-27a-3p was overexpressed in NOA men compared to OA-control individuals. As a consequence, the overexpressed micro-RNA could downregulate directly KDM3A and indirectly TNP1 and PRM1. Therefore, spermatogenesis could be misled and male infertility could be developed. Key words: hsa-miR-27a-3p, Male infertility, KDM3A.

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miRNA‑27a promotes the proliferation and inhibits apoptosis of human pancreatic cancer cells by Wnt/β-catenin pathway

Cited by 13 publications

References 16 publications

miR‐454 performs tumor‐promoting effects in oral squamous cell carcinoma via reducing NR3C2

miR‐454 performs tumor‐promoting effects in oral squamous cell carcinoma via reducing NR3C2

Upregulation of microRNA-23b-3p induced by farnesoid X receptor regulates the proliferation and apoptosis of osteosarcoma cells

Hsa-miR-27a-3p overexpression in men with nonobstructive azoospermia: A case-control study

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