MiRNA-339 targets and regulates ZNF689 to inhibit the proliferation and invasion of gastric cancer cells

Jiang, Haibo; Liu, Yinhua; Hu, Kaifeng; Xia, Yabin; Liang, Linhu; Zhu, Xiaoli; Cheng, Xianfeng

doi:10.21037/tcr-21-994

Cited by 4 publications

(1 citation statement)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a cancer biomarker, miRNA was also closely related to various aspects of normal physiology and pathology, such as the occurrence, proliferation, differentiation, and apoptosis of lung cancer cells. In recent years, miRNA has attracted social research attention ( Jiang et al, 2021 ; Zhang et al, 2022 ). Gou et al (2020) found that inhibition of cardiomyocyte-derived Exo-miR-19a-3p promoted angiogenesis and improved cardiac function in mice with myocardial infarction by targeting HIF-1 α .…”

Section: Discussionmentioning

confidence: 99%

Exosomes derived from M1 macrophages inhibit the proliferation of the A549 and H1299 lung cancer cell lines via the miRNA-let-7b-5p-GNG5 axis

Peng

et al. 2023

PeerJ

View full text Add to dashboard Cite

Background Almost all cells are capable of secreting exosomes (Exos) for intercellular communication and regulation. Therefore, Exos can be used as a natural therapeutic platform to regulate genes or deliver drugs to treat diseases. M1 macrophages inhibit tumor growth by releasing pro-inflammatory factors. This study explored the applicability of M1 macrophage exosomes (M1-Exos) as gene carriers and the effects on GNG5 protein, and further examined whether macrophage repolarization could inhibit tumor activity. Methods M0 macrophages were polarized toward M1 using vitexin. Exos were obtained from M1 macrophages by ultra-centrifugation. The transwell non-contact co-culture system was used to co-culture M1 macrophages with HLF-α human lung epithelial cells or A549 or H1299 lung cancer cells. MTT, scratch, and transwell assays were used to detect the cell viability, migration, and invasion ability of cells in the four groups. Flow cytometry was used to detect the apoptosis rate of each group, and western blot (WB) analysis was performed to detect the change in the expression of proliferation- and apoptosis-related proteins. We screened the differentially expressed microRNAs using quantitative polymerase chain reaction technology. Luciferase reporter analysis was performed to explore the interaction between miRNA and protein. We used Xenografted A549 tumors in nude mice to study the effect of M1-Exos on tumor cell growth in vivo. Results The results showed that, under the M1 macrophage co-culture system, lung cancer cell viability, invasion, and migration ability decreased, and the number of apoptotic cells increased, will all indicators being statistically significant (P < 0.05). The expression levels of PCNA, KI67, and Bcl-2 decreased significantly, but that of Bax increased (P < 0.05). Exosomes can have the same effect on tumor cells as M1 macrophages. Exosomes can transport miR-let-7b-5p to tumor cells, and miR-let-7b-5p can inhibit tumor cell proliferation and promote tumor cell apoptosis by regulating the GNG5 protein level. Conclusions M1-Exos inhibit the proliferation, invasion, and metastasis of lung cancer cells through miRNA-let-7b-5p and GNG5 signaling pathways and inhibit the anti-apoptotic ability of lung cancer cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Exosomes derived from M1 macrophages inhibit the proliferation of the A549 and H1299 lung cancer cell lines via the miRNA-let-7b-5p-GNG5 axis

Peng

et al. 2023

PeerJ

View full text Add to dashboard Cite

show abstract

Knockdown of Long Non-Coding RNA (LncRNA) MAFG Divergent Transcript Inhibits the Tumorigenesis of Hepatocellular Carcinoma Through miR-339-5p/CDC25A Axis

Shi

Guo

et al. 2023

j biomed nanotechnol

View full text Add to dashboard Cite

Hepatocellular carcinoma (HCC) is frequently diagnosed at late stages when curative treatments are no more applicable. Hence, it is essential to explore new strategie sagainst HCC. Long non-coding RNA (LncRNA) MAFG divergent transcript (MAFG-DT) was known to act as a modulator in various cancers. Nevertheless, the role of MAFG-DT in HCC remains unexplored. The Cancer Genome Atlas (TCGA) and bioinformatics analyzes were used to explore MAFG-DT level in HCC. In addition, LncBase was used to explore the downstream miRNA of MAFG-DT, and target scan was applied to analyze the targets of miR-339-5p. Meanwhile, bioinformatics tool was applied to assess the role of CDC25A and miR-339-5p in HCC. Furthermore, CCK8 and transwell assays were applied to assess the cell viability and migration. MAFG-DT level was elevated in HCC tissues. MAFG-DT level was positively correlated with the advanced TNM stage, vascular invasion, histologic grade recurrence and mortality. Kaplan–Meier analysis suggested that MAFG-DT was negatively associated with recurrence-free survival (RFS) and overall survival in HCC. Meanwhile, MAFG-DT was verified to sponge miR-339-5p, and CDC25A was the target mRNA of miR-339-5p. MAFG-DT silencing could significantly inhibit the viability of migration of HCC cells through binding to miR-339-5p. MAFG-DT silencing inhibited the development of HCC through miR-339-5p/CDC25A axis. Thus, our study might supply a new target against HCC.

show abstract

Overview of MicroRNAs as Diagnostic and Prognostic Biomarkers for High-Incidence Cancers in 2021

Sun

Zhao

Wang

et al. 2022

IJMS

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are small non-coding RNAs (ncRNAs) about 22 nucleotides in size, which play an important role in gene regulation and are involved in almost all major cellular physiological processes. In recent years, the abnormal expression of miRNAs has been shown to be associated with human diseases including cancer. In the past ten years, the link between miRNAs and various cancers has been extensively studied, and the abnormal expression of miRNAs has been reported in various malignant tumors, such as lung cancer, gastric cancer, colorectal cancer, liver cancer, breast cancer, and prostate cancer. Due to the high malignancy grade of these cancers, it is more necessary to develop the related diagnostic and prognostic methods. According to the study of miRNAs, many potential cancer biomarkers have been proposed for the diagnosis and prognosis of diseases, especially cancer, thus providing a new theoretical basis and perspective for cancer screening. The use of miRNAs as biomarkers for diagnosis or prognosis of cancer has the advantages of being less invasive to patients, with better accuracy and lower price. In view of the important clinical significance of miRNAs in human cancer research, this article reviewed the research status of miRNAs in the above-mentioned cancers in 2021, especially in terms of diagnosis and prognosis, and provided some new perspectives and theoretical basis for the diagnosis and treatment of cancers.

show abstract

MiRNA-339 targets and regulates ZNF689 to inhibit the proliferation and invasion of gastric cancer cells

Cited by 4 publications

References 31 publications

Exosomes derived from M1 macrophages inhibit the proliferation of the A549 and H1299 lung cancer cell lines via the miRNA-let-7b-5p-GNG5 axis

Exosomes derived from M1 macrophages inhibit the proliferation of the A549 and H1299 lung cancer cell lines via the miRNA-let-7b-5p-GNG5 axis

Knockdown of Long Non-Coding RNA (LncRNA) MAFG Divergent Transcript Inhibits the Tumorigenesis of Hepatocellular Carcinoma Through miR-339-5p/CDC25A Axis

Overview of MicroRNAs as Diagnostic and Prognostic Biomarkers for High-Incidence Cancers in 2021

Contact Info

Product

Resources

About