miRNA-489 as a biomarker in diagnosis and treatment of cervical cancer

Juan, C. De; Hua, Qian; Ruping, Z; Wu, Ting

doi:10.4149/bll_2018_052

Cited by 21 publications

(26 citation statements)

References 20 publications

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“…For instance, miR-338-3p can reduce CC cell growth via regulating MACC1 and MAPK expression [ 24 ]. miR-489 is expressed lowly in CC and reduces the proliferation of CC cells [ 25 ]. miR-221-3p can regulate EMT and metastasis through binding to THBS2 in CC [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

LncRNA SNHG6 enhances the radioresistance and promotes the growth of cervical cancer cells by sponging miR-485-3p

Liu

Liu²,

2020

Cancer Cell Int

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Background: Cervical cancer (CC) is the one of most common malignant gynecological tumors, which is characterized with the high mortality and recurrence rate. Previous studies have elucidated the oncogenic role of small nucleolar RNA host gene 6 (SNHG6) in some types of human cancers, whereas it is unclear whether it functions as an oncogene in CC. This study was aimed at unveiling the role of SNHG6 in CC. Methods: qRT-PCR analysis was implemented to evaluate the expression levels of SNHG6, miR-485-3p and STYX in CC cells. RNA pull down assay and luciferase reporter assay were conducted to verify the interaction between miR-485-3p and SNHG6 or STYX. Functional assays, such as colony formation assay, JC-1 assay and TUNEL assay were applied to detect the biological behaviors of CC cells. The resistance of CC cells to radiation was evaluated by colony formation assay. Results: SNHG6 was expressed at a high level in CC cells. Silenced SNHG6 suppressed cell proliferation but promoted cell apoptosis. Additionally, silenced SNHG6 could sensitize CC cells to radiation treatment. miR-485-3p could bind to both SNHG6 and STYX. Knockdown of miR-485-3p or overexpression of STYX could abolish the effects of SNHG6 silencing on CC cell growth. Conclusions: LncRNA SNHG6 enhances the radioresistance of CC cells and promotes CC cell growth by sponging miR-485-3p to release STYX.

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Section: Discussionmentioning

confidence: 99%

LncRNA SNHG6 enhances the radioresistance and promotes the growth of cervical cancer cells by sponging miR-485-3p

Liu

Liu²,

2020

Cancer Cell Int

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“… 9 A growing body of evidence suggests that the abnormal expression of miRNAs play momentous roles in the tumorigenesis, including CC. 10–12 In this study, using bioinformatics analysis ( http://www.targetscan.org/ ), we found that miR-3150b-3p might bind to TNFRSF11a three prime untranslated region (3’-UTR) to inhibit the expression of TNFRSF11a. Based on the above-mentioned findings, we thus speculated that miR‐3150b-3p via targeting TNFRSF11a inhibited CC development by mediating the p38 MAPK signaling pathway.…”

Section: Introductionmentioning

confidence: 92%

Mir-3150B-3P Inhibits the Proliferation and Invasion of Cervical Cancer Cells by Targeting Tnfrsf11A

ZhiJuan

Wang

2020

Journal of Investigative Medicine

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The objective of this study was to determine the role of miR-3150b-3p in the cervical cancer (CC) progression. Real-time PCR and western blot analysis were conducted to test the expression of miR-3150b-3p, TNFRSF11a and p38 mitogen-activated protein kinase (MAPK) signaling pathway. The interaction between miR-3150b-3p and TNFRSF11a was verified by luciferase assay. Cell proliferation, migration and invasion were determined by CCK-8, wound healing and Transwell assays. In this study, we showed that miR-3150b-3p was significantly downregulated in CC cell lines. Additionally, miR-3150b-3p markedly attenuated the proliferation, migration and invasion of HeLa and SiHa cells. Moreover, we identified TNFRSF11a to be a novel target of miR-3150b-3p in CC cells. Enforced expression of TNFRSF11a abolished the antitumor effect of miR-3150b-3p. Besides, miR-3150b-3p was involved in the regulation of the p38 MAPK signaling pathway. In conclusion, our data suggested that miR-3150b-3p directly targets TNFRSF11a to inactivate the p38 MAPK signaling pathway, thus implicating miR-3150b-3p in the regulation of CC cell growth.

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“…In particular, the mutations of PIK3CA E542K and E545K promote glycolysis and proliferation of CC in vitro and vivo [212]. NBPF1, ARHGAP17, miR-99b, -181a2/181b2, -338, -383, -433 and -489, as well as LncRNA ANRIL, CRNDE, NEAT1 and LINC01305 are involved in the proliferation, invasion, autophagy or EMT via PI3K/AKT pathway [213][214][215][216][217][218][219][220][221][222][223][224]. Currently, only preclinical trials of PI3K inhibitor LY294002 has revealed it significantly radiosensitized CC cell lines in vitro and vivo [225,226], and the terminated clinical trials of AKT inhibitor GSK2141795 (NCT01958112, Table 3) has tried to display a novel treatment approach to patients of CC.…”

Section: Nct02240212mentioning

confidence: 99%

Role of PI3K/AKT pathway in cancer: the framework of malignant behavior

et al. 2020

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Given that the PI3K/AKT pathway has manifested its compelling influence on multiple cellular process, we further review the roles of hyperactivation of PI3K/AKT pathway in various human cancers. We state the abnormalities of PI3K/AKT pathway in different cancers, which are closely related with tumorigenesis, proliferation, growth, apoptosis, invasion, metastasis, epithelial-mesenchymal transition, stem-like phenotype, immune microenvironment and drug resistance of cancer cells. In addition, we investigated the current clinical trials of inhibitors against PI3K/AKT pathway in cancers and found that the clinical efficacy of these inhibitors as monotherapy has so far been limited despite of the promising preclinical activity, which means combinations of targeted therapy may achieve better efficacies in cancers. In short, we hope to feature PI3K/ AKT pathway in cancers to the clinic and bring the new promising to patients for targeted therapies.

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miRNA-489 as a biomarker in diagnosis and treatment of cervical cancer

Cited by 21 publications

References 20 publications

LncRNA SNHG6 enhances the radioresistance and promotes the growth of cervical cancer cells by sponging miR-485-3p

LncRNA SNHG6 enhances the radioresistance and promotes the growth of cervical cancer cells by sponging miR-485-3p

Mir-3150B-3P Inhibits the Proliferation and Invasion of Cervical Cancer Cells by Targeting Tnfrsf11A

Role of PI3K/AKT pathway in cancer: the framework of malignant behavior

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