MiRNA-Based Inspired Approach in Diagnosis of Prostate Cancer

Munteanu, V.C.; Munteanu, Raluca; Onaciu, Anca; Berindan‐Neagoe, Ioana; Petruț, Bogdan; Coman, Ioan

doi:10.3390/medicina56020094

Cited by 13 publications

(7 citation statements)

References 113 publications

(106 reference statements)

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“…Downregulated expression of miR-139-5p promotes prostate cancer progression by targeting SOX5 [19]. Further, miRNAs also can be invoked as potential prognostic biomarkers in prostate cancer [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel six autophagy-related genes signature for the prognostic and a miRNA-related autophagy predictor for anti-PD-1 therapy responses in prostate cancer

Wen

Yue

et al. 2021

BMC Cancer

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Background Autophagy is a highly conserved homeostatic process in the human body that is responsible for the elimination of aggregated proteins and damaged organelles. Several autophagy-related genes (ARGs) contribute to the process of tumorigenesis and metastasis of prostate cancer (PCa). Also, miRNAs have been proven to modulate autophagy by targeting some ARGs. However, their potential role in PCa still remains unclear. Methods An univariate Cox proportional regression model was used to identify 17 ARGs associated with the overall survival (OS) of PCa. Then, a multivariate Cox proportional regression model was used to construct a 6 autophagy-related prognostic genes signature. Patients were divided into low-risk group and high-risk group using the median risk score as a cutoff value. High-risk patients had shorter OS than low-risk patients. Furthermore, the signature was validated by ROC curves. Regarding mRNA and miRNA, 12 differentially expressed miRNAs (DEMs) and 1073 differentially expressed genes (DEGs) were detected via the GEO database. We found that miR-205, one of the DEMs, was negatively regulated the expression of ARG (NKX2–3). Based on STRING analysis results, we found that the NKX2–3 was moderately related to the part of genes among the 6 autophagy-related genes prognostic signature. Further, NKX 2–3 was significantly correlated with OS and some clinical parameters of PCa by cBioProtal. By gene set enrichment analysis (GSEA). Lastly, we demonstrated that the association between NKX2–3 and tumor mutation burden (TMB) and PDCD1 (programmed cell death 1) of PCa. Results We identified that the six ARGs expression patterns are independent predictors of OS in PCa patients. Furthermore, our results suggest that ARGs and miRNAs are inter-related. MiR-205 was negatively regulated the expression of ARG (NKX2–3). Further analysis demonstrated that NKX2–3 may be a potential biomarker for predicting the efficacy of anti-PD-1 therapy in PCa. Conclusions The current study may offer a novel autophagy-related prognostic signature and may identify a promising miRNA-ARG pathway for predicting the efficacy of anti-PD-1 therapy in PCa.

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Section: Introductionmentioning

confidence: 99%

Identification of a novel six autophagy-related genes signature for the prognostic and a miRNA-related autophagy predictor for anti-PD-1 therapy responses in prostate cancer

Wen

Yue

et al. 2021

BMC Cancer

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“…MicroRNAs (miRNAs) are small non-coding RNAs comprising approximately 22 nucleotides that regulate target gene expression by binding to the 3′ untranslated region (UTR) of target mRNAs ( 5 ). Several studies have reported the dysregulation of miRNAs in various types of human cancers, including breast cancer ( 6 ), lung cancer ( 7 , 8 ), and PC ( 9 , 10 ). miRNAs can act as oncogenes or tumor suppressors depending on their target genes.…”

Section: Introductionmentioning

confidence: 99%

miR-31-3p functions as a tumor suppressor by directly targeting GABBR2 in prostate cancer

et al. 2022

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MicroRNAs are key regulators of gene expression in tumorigenesis. In this study, we investigated the tumor-suppressive function of miR-31-3p. Analysis of the Gene Expression Omnibus database revealed that the expression of miR-31-3p in prostate cancer tissues is lower than that in adjacent normal tissues from patients with prostate cancer. Moreover, miR-31-3p induces apoptosis in DU145, PC-3, and LNCap prostate cancer cells, while those transfected with miR-31-3p exhibit significantly decreased cell proliferation, migration, invasiveness, and tumor sphere-forming ability, as determined using the cell counting kit-8, transwell, and sphere-forming assays. Further analysis revealed that GABBR2 is a direct target of miR-31-3p. Within a DU145 xenograft murine model, intratumoral injection of a miR-31-3p mimic suppresses tumor growth. Taken together, the findings of this study suggest that miR-31-3p performs a novel tumor-suppressive function in prostate cancer and may represent a novel target for anti-prostate cancer miRNA therapeutics.

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“…Due to their tumor-specific deregulation, miRNAs also possess diagnostic potential for PCa detection [ 10 , 17 , 18 ]. In addition, miRNAs have the advantage of being generally stable in various body fluids and thus, they are deemed to be useful biomarkers [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

“…Extensive investigations using malignant and non-malignant prostate tissue have shown that certain miRNAs are also differentially expressed in PCa [ 11 , 13 , 14 , 15 , 16 ]. Furthermore, selected miRNAs possessed diagnostic potential when analyzed in urine and/or blood samples from PCa patients and control subjects [ 10 , 11 , 17 , 18 ]. Particularly, the collection of urine is inexpensive, simple, and non-invasive, whereupon the number of prostatic cells and thus the amount of PCa-specific miRNAs in the urine specimens can be increased by DRE.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of MicroRNAs as Non-Invasive Diagnostic Markers in Urinary Cells from Patients with Suspected Prostate Cancer

et al. 2020

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Currently used tumor markers for early diagnosis of prostate cancer (PCa) are often lacking sufficient specificity and sensitivity. Therefore, the diagnostic potential of selected microRNAs in comparison to serum PSA levels and PSA density (PSAD) was explored. A panel of 12 PCa-associated microRNAs was quantified by qPCR in urinary sediments from 50 patients with suspected PCa undergoing prostate biopsy, whereupon PCa was detected in 26 patients. Receiver operating characteristic (ROC) curve analyses revealed a potential for non-invasive urine-based PCa detection for miR-16 (AUC = 0.744, p = 0.012; accuracy = 76%) and miR-195 (AUC = 0.729, p = 0.017; accuracy = 70%). While serum PSA showed an insufficient diagnostic value (AUC = 0.564, p = 0.656; accuracy = 50%) in the present cohort, PSAD displayed an adequate diagnostic performance (AUC = 0.708, p = 0.031; accuracy = 70%). Noteworthy, the combination of PSAD with the best candidates miR-16 and miR-195 either individually or simultaneously improved the diagnostic power (AUC = 0.801–0.849, p < 0.05; accuracy = 76–90%). In the sub-group of patients with PSA ≤ 10 ng/mL (n = 34), an inadequate diagnostic power of PSAD alone (AUC = 0.595, p = 0.524; accuracy = 68%) was markedly surpassed by miR-16 and miR-195 individually as well as by their combination with PSAD (AUC = 0.772–0.882, p < 0.05; accuracy = 74–85%). These findings further highlight the potential of urinary microRNAs as molecular markers with high clinical performance. Overall, these results need to be validated in a larger patient cohort.

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MiRNA-Based Inspired Approach in Diagnosis of Prostate Cancer

Cited by 13 publications

References 113 publications

Identification of a novel six autophagy-related genes signature for the prognostic and a miRNA-related autophagy predictor for anti-PD-1 therapy responses in prostate cancer

Identification of a novel six autophagy-related genes signature for the prognostic and a miRNA-related autophagy predictor for anti-PD-1 therapy responses in prostate cancer

miR-31-3p functions as a tumor suppressor by directly targeting GABBR2 in prostate cancer

Evaluation of MicroRNAs as Non-Invasive Diagnostic Markers in Urinary Cells from Patients with Suspected Prostate Cancer

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