miRNA/epithelial-mesenchymal axis (EMT) axis as a key player in cancer progression and metastasis: A focus on gastric and bladder cancers

Sahib, Ameer S.; Fawzi, Amjid; Zabibah, Rahman S.; Koka, Nisar Ahmad; Khudair, Shaymaa Abdulhameed; Muhammad, Faris Anad; Hamad, Doaa A.

doi:10.1016/j.cellsig.2023.110881

Cited by 4 publications

(1 citation statement)

References 274 publications

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“…In bladder cancer, various microRNAs (miRNAs) have been found to regulate proteins such as Smad7 or Twist1, either promoting or disrupting EMT and metastasis [37]. Understanding the mechanisms underlying EMT is crucial for developing targeted therapies to control cancer metastasis and may prove useful in treatment options going forward.…”

Section: Introductionmentioning

confidence: 99%

Survival and Enrichment Analysis of Epithelial–Mesenchymal Transition Genes in Bladder Urothelial Carcinoma

Ali,

Xiao,

Jacobs

et al. 2023

Genes

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The escalating prevalence of bladder cancer, particularly urothelial carcinoma, necessitates innovative approaches for prognosis and therapy. This study delves into the significance of genes related to epithelial–mesenchymal transition (EMT), a process inherently linked to carcinogenesis and comparatively better studied in other cancers. We examined 1184 EMT-related gene expression levels in bladder urothelial cancer cases through the TCGA dataset. Genes shown to be differentially expressed in relation to survival underwent further network and enrichment analysis to uncover how they might shape disease outcomes. Our in silico analysis revealed a subset of 32 genes, including those significantly represented in biological pathways such as VEGF signaling and bacterium response. In addition, these genes interact with genes involved in the JAK-STAT signaling pathway. Additionally, some of those 32 genes have been linked to immunomodulators such as chemokines CCL15 and CCL18, as well as to various immune cell infiltrates. Our findings highlight the prognostic utility of various EMT-related genes and identify possible modulators of their effect on survival, allowing for further targeted wet lab research and possible therapeutic intervention.

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Section: Introductionmentioning

confidence: 99%

Survival and Enrichment Analysis of Epithelial–Mesenchymal Transition Genes in Bladder Urothelial Carcinoma

Ali,

Xiao,

Jacobs

et al. 2023

Genes

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Nucleic Acid Amplification Circuit‐Based Hydrogel (NACH) Assay for One‐Step Detection of Metastatic Gastric Cancer‐Derived Exosomal miRNA

Seo,

Lim,

Kim

et al. 2024

Advanced Science

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Gastric cancer (GC) is recognized as the fifth most prevalent malignant tumor worldwide. It is characterized by diverse clinical symptoms, treatment responses, and prognoses. In GC prognosis, the promotion of epithelial–mesenchymal transition (EMT) fosters cancer cell invasion and metastasis, thereby triggering the dissemination of tumor cells. This study proposes a nucleic acid amplification circuit‐based hydrogel (NACH) assay for identifying exosomal miRNA derived from metastatic GC. The NACH assay employs the rolling circle amplification method and targets miRNA‐21, a tumor‐related oncogene, and miRNA‐99a, which promotes EMT. Specific amplification probes for each target are immobilized within the hydrogel, enabling a streamlined, one‐step amplification reaction. The NACH assay exhibits a detection limit of 1 fm for miRNA‐21 and miRNA‐99a, thereby enabling rapid and highly sensitive on‐site detection. Performance evaluation using exosomal miRNA extracted from cell culture media, mouse plasma, and human plasma revealed fluorescence intensity patterns similar to those obtained in qRT‐PCR. Furthermore, deploying a custom‐developed portable fluorometer for the NACH assay allows for diagnostic performance assessment and point‐of‐care testing using clinical samples from GC patients. These findings emphasize the potential of the NACH assay to be used as a robust tool for the genetic diagnosis of GC based on exosome detection.

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Therapeutic importance and diagnostic function of circRNAs in urological cancers: from metastasis to drug resistance

Zhang,

Gao,

Fang

et al. 2024

Cancer Metastasis Rev

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miRNA/epithelial-mesenchymal axis (EMT) axis as a key player in cancer progression and metastasis: A focus on gastric and bladder cancers

Cited by 4 publications

References 274 publications

Survival and Enrichment Analysis of Epithelial–Mesenchymal Transition Genes in Bladder Urothelial Carcinoma

Survival and Enrichment Analysis of Epithelial–Mesenchymal Transition Genes in Bladder Urothelial Carcinoma

Nucleic Acid Amplification Circuit‐Based Hydrogel (NACH) Assay for One‐Step Detection of Metastatic Gastric Cancer‐Derived Exosomal miRNA

Therapeutic importance and diagnostic function of circRNAs in urological cancers: from metastasis to drug resistance

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