miRNA expression profiling identifies DSPP regulators in cultured dental pulp cells

Abstract

Abstract. Dentin sialophosphoprotein (DSPP), an important marker of odontoblast differentiation, is a prerequisite for tooth development and mineralization; however, the molecular mechanisms of both temporal and spatial regulation remain unknown. MicroRNAs (miRNAs) provide an additional level of control beyond that of transcription factors, which regulate post-transcriptional control of gene expression. The present study was designed to provide a first attempt at an in-depth analysis of dental pulp cells at various odontoblastic differentiation stages to obtain miRNA differential expression patterns, and to determine the contribution of miRNAs in the expression of DSPP during odontoblast differentiation. Dual luciferase reporter assays and qRT-PCR were used to validate miRNAs identified from bioinformatic analyses to determine whether they were able to regulate the DSPP gene in dental pulp cells cultured in a mineralizing medium. The results presented here suggest that DSPP is regulated post-transcriptionally by mir32, mir885-5p and mir586 during odontoblast differentiation.

Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix‐receptor activity

“…Previous studies had revealed that ITGA5 forced expression by lentivirus or the priming peptide enhanced the osteogenic capacity of human MSC osteoblast (Hamidouche et al, 2009) and human periodontal ligament stem cells (PDLSCs; Wang et al, 2018), which are opposite to the present study. In the present study, however, we had found there was no significant difference between the ITGA5 lentivirus forced expression group and the control group (data not Since the discovery of DPSCs, a mass of research has focused on the proodontogenic effect of different genes or conditions (Cui et al, 2014b;Huang et al, 2011;Ma et al, 2012). However, most of them only reported the proodontogenic effect without further investigation and validation of the involved mechanisms.…”

“…Since the discovery of DPSCs, a mass of research has focused on the proodontogenic effect of different genes or conditions (Cui et al, ; Huang et al, ; Ma et al, ). However, most of them only reported the proodontogenic effect without further investigation and validation of the involved mechanisms.…”

Stem Cells from Dental Pulp: What Epigenetics Can Do with Your Tooth

“…On the other hand, it was found that several miRNAs, including miR-32, miR-885-5, and miR-586, are differentially expressed during the odontoblast differentiation of DPCs (Figure 4B ). Although these miRNAs can be found in both undifferentiated and differentiated DPCs, their levels decrease considerably under mineralization induction, which corresponds with the highest levels of DSPP expression, a prerequisite for initiating mineralization events (Huang et al, 2011 ).…”

A comparison of microRNA profiles between rat incisors and molars

“…This corresponds to recent reports indicated this miRNA become lowest level compare to the other miRNAs (mir-32, mir-885-5p), when DSPP was the peak level. Moreover, the undetected level might cause by this candidate miRNA derived from study that has experiment on human dental pulp cells (56).…”

“…In addition to the well-known signaling factor, according to enamel mineralization, Fan et al reported that miR-224 can bind to the 30UTRs of Slc4a4 and cystic fibrosis transmembrane conductance regulator mRNAs, which are highly expressed during ameloblast differentiation as ion transporters to maintain pH homeostasis and support enamel mineralization (54). About dentinogenesis, Huang et al, identified that the Dentin sialophosphoprotein (DSPP), an important marker (55), expression of dental pulp cells is regulated post-transcriptionally by miR-32, miR-885-5p, and miR-586 during odontoblast differentiation, although the complex regulatory network between miRNA and mRNAs remains to be determined (56). In additional studies, Heair et al, also found that miR-665 as a potential repressor of odontoblast maturation by contribute to the alteration in the chromatin status of DSPP and Dmp1 by targeting Kat6a expression and also directly targeted Dlx3 mRNA and decreased DLX3 expression and its downstream targets (Runx2,Osx,DSPP) in mouse odontoblasts (57).…”