miRNA expression profiling in formalin-fixed and paraffin-embedded placental tissue samples from pregnancies with severe preeclampsia

Noack, F.; Ribbat‐Idel, Julika; Thorns, Christoph; Chiriac, Andrea; Axt-Fliedner, R; Diedrich, K.; Feller, Alfred C.

doi:10.1515/jpm.2011.012

Cited by 62 publications

(43 citation statements)

References 27 publications

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“…Several reports (Pineles et al 2007, Roman et al 2008, Zhu et al 2009, Enquobahrie et al 2011, Mayor-Lynn et al 2011, Noack et al 2011 have illustrated the differential expression of placental miRNAs in PE patients. However, except for miR-210 (elevated in preeclamptic placenta) (Pineles et al 2007, Zhu et al 2009, Enquobahrie et al 2011, miR-181a (elevated in preeclamptic placenta) , Zhu et al 2009), and miR-1 (decreased in preeclamptic placenta) (Roman et al 2008, Zhu et al 2009, Enquobahrie et al 2011, there was little overlap among these data; this could have resulted from differences in the sample collections (including the gestational ages of the placentas and the processing of the placentas), profiling methods and patients' ethnicities ).…”

Section: Discussionmentioning

confidence: 99%

Circulating microRNAs are elevated in plasma from severe preeclamptic pregnancies

Zhou²,

Lin³

et al. 2012

Reproduction

173

137

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Until recently, the molecular pathogenesis of preeclampsia (PE) remained largely unknown. Reports have shown that circulating microRNAs (miRNAs) are promising novel biomarkers for cancer, pregnancy, tissue injury, and other conditions. The objective of this study was to identify differentially expressed miRNAs in plasma from severe preeclamptic pregnancies compared with plasma from normal pregnancies. By mature miRNA microarray analysis, 15 miRNAs, including 13 up-and two downregulated miRNAs, were screened to be differentially expressed in plasma from women with severe PE (sPE). Seven miRNAs, namely miR-24, miR-26a, miR-103, miR-130b, miR-181a, miR-342-3p, and miR-574-5p, were validated to be elevated in plasma from severe preeclamptic pregnancies by real-time quantitative stem-loop RT-PCR analysis. Gene ontology and pathway enrichment analyses revealed that these miRNAs were involved in specific biological process categories (including regulation of metabolic processes, regulation of transcription, and cell cycle) and signaling pathways (including the MAP kinase signaling pathway, the transforming growth factor-b signaling pathway, and pathways in cancer metastasis). This study presents, for the first time, the differential expression profile of circulating miRNAs in sPE patients. The seven elevated circulating miRNAs may play critical roles in the pathogenesis of sPE, and one or more of them may become potential markers for diagnosing sPE.

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Section: Discussionmentioning

confidence: 99%

Circulating microRNAs are elevated in plasma from severe preeclamptic pregnancies

Zhou²,

Lin³

et al. 2012

Reproduction

173

137

View full text Add to dashboard Cite

show abstract

“…Enquobahrie et al (2011) further confirmed the downregulation of previously identified miRNA candidates (miR-1, miR-328, miR-139-5p, miR-500, and miR-1247) and found two novel miRNA candidates to be downregulated in pregnancies complicated by PE (miR-584 and miR-34c-5p). Noack et al (2011) identified another six miRNAs to be overexpressed in five cases with severe PE who delivered at gestational age ranging from 29 to 37 weeks (let-7b, miR-302*, miR-104, miR-128a, miR-182*, and miR-133b).…”

Section: Differential Expression Of Mirnas Between Pre-eclamptic and mentioning

confidence: 99%

Extracellular Nucleic Acids in Maternal Circulation as Potential Biomarkers for Placental Insufficiency

Hromadníková

2012

DNA and Cell Biology

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“…Although different hypotheses have been put forth, the underlying etiology of preeclampsia is unknown (32). Analysis of miRNAs from formalin-fixed placental specimens of preeclampsia patients has demonstrated altered miRNA profiles (33)(34)(35). Deep sequencing of miRNAs obtained from placental tissues suggests that, in the setting of preeclampsia, the isomiR profile is altered with increased 3′ end addition of an adenosine (36).…”

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confidence: 99%

Comprehensive profiling of circulating microRNA via small RNA sequencing of cDNA libraries reveals biomarker potential and limitations

Williams

Ben‐Dov

Elias

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

325

268

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We profiled microRNAs (miRNAs) in cell-free serum and plasma samples from human volunteers using deep sequencing of barcoded small RNA cDNA libraries. By introducing calibrator synthetic oligonucleotides during library preparation, we were able to calculate the total as well as specific concentrations of circulating miRNA. Studying trios of samples from newborn babies and their parents we detected placental-specific miRNA in both maternal and newborn circulations and quantitated the relative contribution of placental miRNAs to the circulating pool of miRNAs. Furthermore, sequence variation in the placental miRNA profiles could be traced to the specific placenta of origin. These deep sequencing profiles, which may serve as a model for tumor or disease detection, allow us to define the repertoire of miRNA abundance in the circulation and potential uses as biomarkers.pregnancy | cancer

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miRNA expression profiling in formalin-fixed and paraffin-embedded placental tissue samples from pregnancies with severe preeclampsia

Cited by 62 publications

References 27 publications

Circulating microRNAs are elevated in plasma from severe preeclamptic pregnancies

Circulating microRNAs are elevated in plasma from severe preeclamptic pregnancies

Extracellular Nucleic Acids in Maternal Circulation as Potential Biomarkers for Placental Insufficiency

Comprehensive profiling of circulating microRNA via small RNA sequencing of cDNA libraries reveals biomarker potential and limitations

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