miRNA profiles of canine cutaneous mast cell tumours with early nodal metastasis and evaluation as potential biomarkers

Zamarian, Valentina; Ferrari, Roberta; Stefanello, D.; Ceciliani, Fabrizio; Grieco, Valeria; Minozzi, Giulietta; Chiti, Lavinia Elena; Arigoni, Maddalena; Calogero, Raffaele; Lecchi, Cristina

doi:10.1038/s41598-020-75877-x

Cited by 8 publications

(12 citation statements)

References 66 publications

(84 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the current study, the potential of saliva as a reliable minimally invasive biosample for the quantification of MCT-associated miRNAs in dogs was investigated for the first time. Three MCT-associated miRNAs, miR-21, miR-379, and miR-885, previously reported to discriminate between cutaneous MCT-affected and healthy dogs in formalin-fixed paraffin-embedded primary tumors, 34 was quantified in saliva samples of healthy and MCT affected dogs to investigate their potential as minimally invasive biomarkers. The three salivary miRNAs were quantified using a qPCR approach, and the results showed that only salivary miR-21 was modulated in the presence of the subcutaneous MCT.…”

Section: Discussionmentioning

confidence: 99%

“…4 MiRNA-126 is up-regulated in some epithelial and nonepithelial neoplasms, like MCT, 7 and the amounts of miR-21, miR-379, and miR-885 in the tumor are able to predict the spread to lymph nodes in MCT-affected dogs. 34 Increased levels of miR-21 have been observed in the saliva of several cancer-affected human patients, suggesting its role as an oncomiRNA 19 promoting tumor progression. 3 The present study confirmed that miR-21 in the saliva of subcutaneous MCT-affected dogs is also higher compared with healthy and cutaneous MCT-affected dogs.…”

Section: Discussionmentioning

confidence: 99%

“…7,10,25,30 Therefore, the ability to discriminate the nonmetastatic/premetastatic (HN0-1 class) from the early-metastatic/overt-metastatic (HN2-3 class) lymph nodes may provide a step forward in obtaining more accurate staging. So far, few studies using transcriptomic 17 and miRNomic 34 approaches have investigated epigenetic changes in MCTs. Fenger 5 and colleagues reported overexpressed miR-9 in canine high-grade MCTs.…”

Section: Discussionmentioning

confidence: 99%

“…5,30,32 Previous studies have detected some MCT-associated miRNAs in plasma and the primary tumor. 4,7,34 The miRNA profile of primary MCTs in formalinfixed paraffin-embedded samples has been previously investigated and suggest that three dysregulated miRNAs, miR-21, miR-379, and miR-885, are able to identify lymph node involvement and discriminate between non-metastatic (HN0-1) and metastatic (HN2-3) tumors. 34 The aim of the present study was to demonstrate the potential of saliva as a minimally invasive biological fluid for the detection and quantification of tumor-associated miRNAs suitable for diagnosing and lymph node staging of canine MCT.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Salivary miR-21 is a potential biomarker for canine mast cell tumors

et al. 2022

Self Cite

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are a class of noncoding RNA molecules playing a crucial role in tumor modulation targeting mRNA. This study aimed to validate the diagnostic potential of a panel of 3 miRNAs previously identified in canine mast cell tumors (MCTs), miR-21, miR-379, and miR-885, as markers of lymph node involvement in terms of histological absence (nonmetastatic: HN0; premetastatic: HN1) and presence (early-metastatic: HN2; overt-metastatic: HN3) of metastasis, in the saliva of mast cell tumor (MCT)-affected dogs by quantitative polymerase chain reaction (PCR). Forty-seven saliva samples were analyzed: 36 from MCT-affected dogs (12 subcutaneous [3 HN0-1 and 9 HN2-3] and 24 cutaneous [9 HN0-1 and 15 HN2-3—MCT]) and 11 from healthy dogs. MCT-group effects were investigated using analysis of variance (ANOVA). The origin of the tumor affected the expression of salivary miR-21 ( P = .011) with an increase in cases with subcutaneous MCTs compared with the healthy group ( P = .0005) and those with cutaneous MCTs ( P = .004). Salivary miR-21 was higher in the HN2-3 class compared with the healthy group ( P = .004). Salivary miR-885 was not affected by the presence of MCT, while miR-379 was not detected in saliva. The diagnostic potential of salivary miR-21 in discriminating MCT-affected dogs from the healthy group (AUC = 0.8917), cutaneous from subcutaneous (AUC = 0.8111), and subcutaneous HN0-1 (AUC = 0.7250) and HN2-3 (AUC = 0.9750) classes from healthy samples was demonstrated by receiver operating characteristic curve analysis. Overall, salivary miR-21 was identified as a promising tool, representing a novel approach to detecting MCT-associated epigenetic alterations in a minimally invasive manner.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Salivary miR-21 is a potential biomarker for canine mast cell tumors

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The miRNAs profile of canine MCT has been previously characterized in formalin-fixed paraffin-embedded samples pointing out that a panel of three miRNAs, miR-21-5p, miR-379, and miR-885, have a good efficiency in discriminating healthy and MCT affected dogs, and MCT affected dogs with and without nodal metastasis, suggesting an epigenetic role in tumor progression ( 26 ). Recently, miR-21-5p has been identified as a potential biomarker in the saliva of MCT-affected dogs ( 27 ).…”

Section: Introductionmentioning

confidence: 99%

Plasma small extracellular vesicles from dogs affected by cutaneous mast cell tumors deliver high levels of miR-21-5p

Zamarian¹,

Stefanello²,

Ferrari³

et al. 2023

Front. Vet. Sci.

Self Cite

View full text Add to dashboard Cite

Small extracellular vesicles (sEV) are a class of extracellular vesicles (30–150 nm), delivering molecules including proteins, metabolites, and microRNAs (miRNAs), involved in physiological intercellular crosstalk and disease pathogenesis. The present pilot study aims are (I) to develop an easy and fast protocol for the isolation of sEV from plasma of mast cell tumor (MCT)-affected dogs; (II) to evaluate if miR-21-5p (sEV-miR-21-5p), a miRNA overexpressed by MCT, is associated with sEV. Seventeen dogs have been enrolled in the study: 4 healthy and 13 (6 with and 7 without nodal metastasis) MCT-affected dogs. sEV were isolated using size exclusion chromatography (SEC) (IZON column 35nm) and were characterized by Western blot, Nanoparticle tracking analysis, and transmission electron microscopy. sEV-miR-21-5p was quantified using digital PCR. sEV expressed the specific markers CD9 and TSG101, and a marker of mast cell tryptase. The sEV mean concentration and size were 2.68E + 10 particles/ml, and 99.6 nm, 2.89E + 10 particles/ml and 101.7 nm, and 3.21E + 10 particles/ml and 124 nm in non-metastatic, nodal metastatic, and healthy samples, respectively. The comparative analysis demonstrated that the level of sEV-miR-21-5p was significantly higher in dogs with nodal metastasis compared to healthy (P = 0.038) and without nodal metastasis samples (P = 0.007). In conclusion, the present work demonstrated that a pure population of sEV can be isolated from the plasma of MCT-affected dogs using the SEC approach and that the level of sEV-miR-21-5p is higher in nodal metastatic MCT-affected dogs compared with healthy and MCT-affected dogs without nodal involvement.

show abstract