miRNA858b Inhibits Proanthocyanidin Accumulation by Repression ofDkMYB19andDkMYB20in Persimmon

Abstract

13Persimmon proanthocyanidin (PA) biosynthetic had been reported to be 14 regulated by several transcription factors, but the miRNAs function involved in this 15 process was poorly understood. We identified a miRNA858b that putatively targeted 16 two R2R3-MYB transcription factors, DkMYB19/DkMYB20. Transcript accumulation 17 of DkMYB19/DkMYB20 and miRNA858b showed contrasting divergent expression 18 patterns during fruit development. DkMYB19/DkMYB20 were confirmed to be 19 localized in the nucleus. The interaction between miRNA858b and 20 DkMYB19/DkMYB20 were experimentally validated by 5' RNA ligase-mediated 21 RACE and LUC enzyme activity detection. Overexpression of miRNA858b led to the 22 33 Keywords 34 Persimmon 35 Proanthocyanidin 36 microRNA 37 MYB 38 39 157 provided evidence to that miRNA858b-MYB played an important role in the 158 regulation of PA biosynthesis in persimmon.159

MiR858b Inhibits Proanthocyanidin Accumulation by the Repression of DkMYB19 and DkMYB20 in Persimmon

“…The authors gratefully acknowledge the technical platform of Sangon Biotech for assistance in vector design, primers, and genes synthesis and sequencing. This manuscript has been released as a pre-print at (bioRxiv) ( Yang et al, 2019 ).…”