MiRNAs Predict the Prognosis of Patients with Triple Negative Breast Cancer: A Meta-Analysis

Liu, Yanli; Zhang, Yuchao; Li, Qingfu; Li, Junfang; Ma, Xinyan; Xing, Jie; Rong, Shouhua; Wu, Zhisheng; Tian, Yuan; Li, Jing; Jia, Liting

doi:10.1371/journal.pone.0170088

Cited by 19 publications

(13 citation statements)

References 35 publications

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“…The demonstration that miRNAs are stable and can be extracted from tissues, blood, and other body fluid without degradation makes them ideal biomarkers for diagnosis and prognosis in breast cancer and other solid tumors. A meta-analysis of studies designed to determine the prognostic role of plasma miRNAs in patients with triple negative breast cancer showed a correlation between high circulating miRNA expression and lower disease-free survival, relapse-free survival and distant metastasis-free survival rates [ 17 ]. Sochor and collaborators [ 18 ] found that high-risk patients (classified as TNBC, HER2, highly proliferative or with positive node involvement) expressed higher levels in plasma of miRNAs related to cancer (oncomiRs), including miR-2.…”

Section: Discussionmentioning

confidence: 99%

Prognostic role of elevated mir-24-3p in breast cancer and its association with the metastatic process

et al. 2018

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MicroRNAs have been shown to play important roles in breast cancer progression and can serve as biomarkers. To assess the prognostic role of a panel of miRNAs in breast cancer, we collected plasma prospectively at the time of initial diagnosis from 1,780 patients with stage I-III breast cancer prior to definitive treatment. We identified plasma from 115 patients who subsequently developed distant metastases and 115 patients without metastatic disease. Both groups were matched by: age at blood collection, year of blood collection, breast cancer subtype, and stage. The median follow up was 3.4 years (range, 1-9 years). We extracted RNA from plasma and analyzed the expression of 800 miRNAs using Nanostring technology. We then assessed the expression of miRNAs in primary and metastatic breast cancer samples from The Cancer Genome Atlas (TCGA). We found that, miR-24-3p was upregulated in patients with metastases, both in plasma and in breast cancer tissues. Patients whose primary tumors expressed high levels of miR-24-3p had a significantly lower survival rate compared to patients with low mir-24-3p levels in the TCGA cohort (n=1,024). RNA-Seq data of the samples with the highest miR-24-3p expression versus those with the lowest miR-24-3p in the TCGA cohort identified a specific gene expression signature for those tumors with high miR-24-3p. Possible target genes for miR-24-3p were predicted based on gene expression and binding site, and their effects on cancer pathways were evaluated. Cancer, breast cancer and proteoglycans were the top three pathways affected by miR-24-3p overexpression.

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Section: Discussionmentioning

confidence: 99%

Prognostic role of elevated mir-24-3p in breast cancer and its association with the metastatic process

et al. 2018

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“…NGS has expanded our ability to investigate RNA expression outside of the coding genome. While many studies have implicated miRNAs that associate with prognosis, little is known about the expression pattern of non-coding RNAs in triple-negative breast cancers [ 52 , 53 ]. Using TNBC cell line models, we performed a global analysis of small non-coding RNAs.…”

Section: Discussionmentioning

confidence: 99%

The Landscape of Small Non-Coding RNAs in Triple-Negative Breast Cancer

Guo

Wang

et al. 2018

Genes

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Triple-negative breast cancer (TNBC) is an operational term for breast cancers lacking targetable estrogen receptor expression and HER2 amplifications. TNBC is, therefore, inherently heterogeneous, and is associated with worse prognosis, greater rates of metastasis, and earlier onset. TNBC displays mutational and transcriptional diversity, and distinct mRNA transcriptional subtypes exhibiting unique biology. High-throughput sequencing has extended cancer research far beyond protein coding regions that include non-coding small RNAs, such as miRNA, isomiR, tRNA, snoRNAs, snRNA, yRNA, 7SL, and 7SK. In this study, we performed small RNA profiling of 26 TNBC cell lines, and compared the abundance of non-coding RNAs among the transcriptional subtypes of triple negative breast cancer. We also examined their co-expression pattern with corresponding mRNAs. This study provides a detailed description of small RNA expression in triple-negative breast cancer cell lines that can aid in the development of future biomarker and novel targeted therapies.

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“…Indeed, BC EVs have been found to induce the proliferation, migration and invasion of recipient cells, thus enhancing disease progression ( 15 – 17 ). Exploiting circulating miRNAs for the diagnosis and prognosis of TNBC patients might yield powerful biomarkers that are easily accessible via liquid biopsy ( 18 ).…”

Section: Introductionmentioning

confidence: 99%

Glucocorticoid receptor overexpression slightly shifts microRNA expression patterns in triple-negative breast cancer

et al. 2018

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Triple-negative breast cancer (TNBC) is a particularly aggressive subtype of breast cancer with limited options for clinical intervention. As with many solid tumors, TNBC is known to promote invasiveness and metastasis by secreting extracellular vesicles (EVs) capable of modulating the behaviour of recipient cells. Recent investigations have demonstrated that high expression levels of glucocorticoid receptor (GR) in TNBC are linked to therapy resistance, higher recurrence rates and increased mortality. In addition to activating protein-coding genes, GR is also involved in the expression of short non-coding RNAs including microRNAs (miRNAs or miRs). The molecular mechanisms responsible for the oncogenic effects of GR on TNBC have yet to be fully elucidated; however, emerging evidence suggests that miRNAs may play a pivotal role in tumorigenesis and metastasis. Thus, the aim of this study was to identify GR-regulated cellular and vesicular miRNAs that might contribute to the particularly oncogenic phenotype of TNBC with a high GR expression. We analyzed miRNA profiles of three TNBC cell lines using an in vitro model of GR overexpression. Next-generation sequencing revealed minor, cell line-specific changes in cellular miRNA expression, whereas vesicular miRNAs were not significantly regulated by GR. Additionally, the analysis of predicted miRNA targets failed to establish a causal link between GR-induced miRNA expression and oncogenic signaling. On the whole, given that GR influences miRNA profiles to only a small degree, other mechanisms are more likely to be responsible for the increased mortality of patients with TNBC with a high GR expression.

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MiRNAs Predict the Prognosis of Patients with Triple Negative Breast Cancer: A Meta-Analysis

Cited by 19 publications

References 35 publications

Prognostic role of elevated mir-24-3p in breast cancer and its association with the metastatic process

Prognostic role of elevated mir-24-3p in breast cancer and its association with the metastatic process

The Landscape of Small Non-Coding RNAs in Triple-Negative Breast Cancer

Glucocorticoid receptor overexpression slightly shifts microRNA expression patterns in triple-negative breast cancer

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