miRNAs promote generation of porcine-induced pluripotent stem cells

Ma, Kuiying; Song, Guangqi; An, Xinglan; Fan, Anran; Tan, Wenxi; Tang, Bo; Zhang, Xueming; Li, Ziyi

doi:10.1007/s11010-013-1942-x

Cited by 29 publications

(23 citation statements)

References 42 publications

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“…The iPS technique allows researchers to establish stem cells by introducing several reprogramming factors into the cells [Takahashi and Yamanaka, ; Takahashi et al, ]. The porcine iPS cells have been established by various scientists, and the characteristics of these stem cells were reported in ∼20 articles [Esteban et al, , ; Ezashi et al, , ; Wu et al, ; West et al, ; Huang et al, ; Ruan et al, ; Aravalli et al, ; Cheng et al, ; Liu et al, ; Rodriguez et al, ; Fujishiro et al, ; Ji et al, ; Kues et al, ; Li et al, ; Park et al, ,; Petkov et al, ; Ma et al, ]. The morphology of the porcine iPS cells is close to that of their human counterparts, which was described as the “primed state” in previous reports.…”

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confidence: 99%

Expression of Six Proteins Causes Reprogramming of Porcine Fibroblasts Into Induced Pluripotent Stem Cells With Both Active X Chromosomes

et al. 2016

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mentioning

confidence: 99%

Expression of Six Proteins Causes Reprogramming of Porcine Fibroblasts Into Induced Pluripotent Stem Cells With Both Active X Chromosomes

et al. 2016

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“…These miR-derived iPS cells were indistinguishable from conventionally generated iPS cells. MicroR reprogramming seems to have advantages for cellular reprogramming [114][115][116] , for example it avoids the need of transducing proto-oncogenic transcription factors [117,118] . However, it needs to be assessed whether this approach will be successful in other species, since the underlying mechanisms are not well understood [119] .…”

Section: Mrnas and Micrornasmentioning

confidence: 99%

Induced pluripotent stem cells: Mechanisms, achievements and perspectives in farm animals

Kumar

Anand

Kues

2015

WJSC

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Pluripotent stem cells are unspecialized cells with unlimited self-renewal, and they can be triggered to differentiate into desired specialized cell types. These features provide the basis for an unlimited cell source for innovative cell therapies. Pluripotent cells also allow to study developmental pathways, and to employ them or their differentiated cell derivatives in pharmaceutical testing and biotechnological applications. Via blastocyst complementation, pluripotent cells are a favoured tool for the generation of genetically modified mice. The recently established technology to generate an induced pluripotency status by ectopic co-expression of the transcription factors Oct4, Sox2, Klf4 and c-Myc allows to extending these applications to farm animal species, for which the derivation of genuine embryonic stem cells was not successful so far. Most induced pluripotent stem (iPS) cells are generated by retroviral or lentiviral transduction of reprogramming factors. Multiple viral integrations into the genome may cause insertional mutagenesis and may increase the risk of tumour formation. Non-integration methods have been reported to overcome the safety concerns associated with retro and lentiviral-derived iPS cells, such as transient expression of the reprogramming factors using episomal plasmids, and direct delivery of reprogramming mRNAs or proteins. In this review, we focus on the mechanisms of cellular reprogramming and current methods used to induce pluripotency. We also highlight problems associated with the generation of iPS cells. An increased understanding of the fundamental mechanisms underlying pluripotency and refining the methodology of iPS cell generation will have a profound impact on future development and application in regenerative medicine and reproductive biotechnology of farm animals.

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“…Other pluripotent factors such as Nanog and Lin28 have been overexpressed in the reprogramming process to derive piPSCs with high quality and efficiency [57]. Combination pluripotent factors and microRNAs including miR-302a, miR-302b and miR-200c have been reported to improve the efficiency of porcine somatic cell reprogramming [65]. Lentiviral and retrovirally mediated reprogramming methods have been used more frequently to generate piPSCs [50,51,52,55].…”

Section: Generation Of Escs and Ipscs In Pigsmentioning

confidence: 99%

“…Porcine iPSCs were pluripotent due to their ability to differentiate into cell types representative of the three germ layers through both in vitro EBs and in vivo teratomas [50,51,52,54,55,65]. They had a normal karyotype, but pluripotent marker expression was not stable.…”

Section: Characteristics Of Porcine Pscsmentioning

confidence: 99%

Progress, problems and prospects of porcine pluripotent stem cells

Wang¹,

Pei²,

Li³

et al. 2014

Front. Agr. Sci. Eng.

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Pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced PSCs (iPSCs), can differentiate into cells of the three germ layers, suggesting that PSCs have great potential for basic developmental biology research and wide applications for clinical medicine. Genuine ESCs and iPSCs have been derived from mice and rats, but not from livestock such as the pig-an ideal animal model for studying human disease and regenerative medicine due to similarities with human physiologic processes. Efforts to derive porcine ESCs and iPSCs have not yielded high-quality PSCs that can produce chimeras with germline transmission. Thus, exploration of the unique porcine gene regulation network of preimplantation embryonic development may permit optimization of in vitro culture systems for raising porcine PSCs. Here we summarize the recent progress in porcine PSC generation as well as the problems encountered during this progress and we depict prospects for generating porcine naive PSCs.

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miRNAs promote generation of porcine-induced pluripotent stem cells

Cited by 29 publications

References 42 publications

Expression of Six Proteins Causes Reprogramming of Porcine Fibroblasts Into Induced Pluripotent Stem Cells With Both Active X Chromosomes

Expression of Six Proteins Causes Reprogramming of Porcine Fibroblasts Into Induced Pluripotent Stem Cells With Both Active X Chromosomes

Induced pluripotent stem cells: Mechanisms, achievements and perspectives in farm animals

Progress, problems and prospects of porcine pluripotent stem cells

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