miRNAs Regulate miRNAs: Coordinated Transcriptional and Post-Transcriptional Regulation

Tuccoli, Andrea; Poliseno, Laura; Rainaldi, Giuseppe

doi:10.4161/cc.5.21.3422

Cited by 34 publications

(23 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…29 Finally, a report from Tuccoli and colleagues focused on the direct effect of miRNA-133 not only on muscle cells, but also on endothelial cells. 38 In this study, an analysis of miRNA expression in HUVECs revealed that miRNA-133, when overexpressed, has angiogenic factor receptors as putative targets, thus demonstrating a potential role of this miRNA even in the control of the vascular angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

A Unique MicroRNA Signature Associated With Plaque Instability in Humans

Cipollone

Felicioni

Sarzani

et al. 2011

Stroke

159

View full text Add to dashboard Cite

Background and Purpose-Atherosclerotic plaque rupture is considered the most important mechanism that underlies the onset of stroke, myocardial infarction, and sudden death. Several evidences demonstrated the pivotal role of inflammatory processes in plaque destabilization. MicroRNAs (miRNAs) are small endogenous RNAs and represent a new important class of gene regulators. Nevertheless, no data exist about the expression profile of miRNAs in atherosclerotic plaques. Thus, the aim of this study was to investigate the expression level of miRNAs in human plaques and to correlate it with clinical features of plaque destabilization. Methods-Two separate groups of plaques were collected from patients who underwent carotid endarterectomy in Chieti (nϭ15) and Ancona (nϭ38) Hospitals. All the plaques were subdivided in symptomatic (nϭ22) and asymptomatic (nϭ31) according to the presence/absence of stroke. Results-First, on the plaques collected at Chieti Hospital, we performed large-scale analysis of miRNA expression.Between the 41 miRNAs examined, we discovered profound differences in the expression of 5 miRNAs (miRNA-100, miRNA-127, miRNA-145, miRNA-133a, and miRNA-133b) in symptomatic versus asymptomatic plaques. Remarkably, when we repeated the analysis on the Ancona plaque subset, all these 5 miRNAs confirmed to be significantly more expressed in the symptomatic plaques. Finally, in vitro experiments on endothelial cells transfected with miRNA-145 and miRNA-133a confirmed the importance of these miRNAs in the modulation of stroke-related proteins. Conclusions-These results are the first to report alterations in the expression of specific miRNAs in human atherosclerotic plaques and suggest that miRNAs may have an important role in regulating the evolution of atherosclerotic plaque toward instability and rupture. Furthermore, by identifying the specific miRNA signature for stroke now, we are able to use computer algorithms to identify previously unrecognized molecular targets. (Stroke. 2011;42:2556-2563.)

show abstract

Section: Discussionmentioning

confidence: 99%

A Unique MicroRNA Signature Associated With Plaque Instability in Humans

Cipollone

Felicioni

Sarzani

et al. 2011

Stroke

159

View full text Add to dashboard Cite

show abstract

“…104 In addition, selective depletion of miR-221 and miR-222 in vitro was shown to critically affect the specific endothelial cell miR-expression pattern: 9 miRs were upregulated, whereas 23 were found downregulated, thus predicting the presence of a complex miR network involved in transcription factor control. 105 Of note, the same authors showed that the muscle-specific miR, miR-133a, was also overexpressed, thus anticipating a potential role of this miR even in the control of the vascular system.…”

Section: Angiogenesismentioning

confidence: 99%

Emerging Role of MicroRNAs in Cardiovascular Biology

Latronico

Catalucci

Condorelli

2007

Circulation Research

269

182

View full text Add to dashboard Cite

Abstract-The heart is among the most conserved organs of the body and is susceptible to defects more than any other organ. Heart malformations, in fact, occur in roughly 1% of newborns. Moreover, cardiovascular disease arising during adult life is among the main causes of morbidity and mortality in developed countries. It is not surprising, therefore, that much effort is being channeled into understanding the development, physiology, and pathology of the cardiovascular system. MicroRNAs, a newly discovered class of small ribonucleotide-based regulators of gene expression, are being implicated in an increasing number of biological processes, and the study of their role in cardiovascular biology is just beginning. Here, we briefly overview microRNAs in general and report on the recent findings regarding their importance for the heart and vasculature, in particular. The new insights that are being gained will permit not only a greater understanding of cardiovascular pathologies but also, hopefully, the development of novel therapeutic strategies.

show abstract

“…However, only miR-221 modulated p21 WAF1 and p27 kip1 expression. Depleting both miR-221 and miR-222 in human umbilical vein endothelial cells further modulated the expression of a distinct set of 32 miRNAs, with nine up-regulated and 23 down-regulated miRNAs (39), suggesting that the functional effect seen with the manipulation of one miRNA may be the result of secondary effects on downstream mRNA or protein expression (39).…”

Section: Original Researchmentioning

confidence: 99%

Airway Smooth Muscle Hyperproliferation Is Regulated by MicroRNA-221 in Severe Asthma

Perry

Baker

Gibeon

et al. 2014

Am J Respir Cell Mol Biol

135

129

View full text Add to dashboard Cite

Increased airway smooth muscle (ASM) mass is a feature of asthmatic airways, and could result from augmented proliferation. We determined whether proliferation and IL-6 release are abnormal in ASM cells (ASMCs) from patients with severe asthma, and whether these features could be mediated by microRNA-221 and microRNA-222, through modulation of the cyclin-dependent kinase inhibitors, p21 WAF1 and p27 kip1 . ASMCs cultured from bronchial biopsies of healthy subjects and patients with nonsevere or severe asthma were studied. Proliferation was measured by the incorporation of bromodeoxyuridine and IL-6 by ELISA. FCS and transforming growth factor (TGF)-b caused greater proliferation and IL-6 release in patients with severe compared with nonsevere asthma and normal subjects. FCS 1 TGF-b inhibited p21 WAF1 and p27 kip1 expression, and increased microRNA-221 (miR-221) expression in ASMCs from individuals with severe asthma. miR-221, and not miR-222, mimics the increased proliferation and IL-6 release induced by FCS 1 TGF in healthy ASM, whereas in patients with severe asthma, the inhibition of miR-221, but not miR-222, inhibited proliferation and IL-6 release. miR-221 inhibition led to the increased expression of FCS 1 TGF-b-induced p21 WAF1 and p27 kip1 . Dexamethasone suppressed proliferation in healthy subjects, but not in subjects with asthma. IL-6 was less suppressible by dexamethasone in patients with nonsevere and severe asthma, compared with healthy subjects. miR-221 did not influence the effects of dexamethasone. ASM from patients with severe asthma shows greater proliferation and IL-6 release than in patients with nonsevere asthma, but both groups show corticosteroid insensitivity. miR-221 regulates p21WAF1 and p27 kip1 expression levels. Furthermore, miR-221 regulates the hyperproliferation and IL-6 release of ASMCs from patients with severe asthma, but does not regulate corticosteroid insensitivity.

show abstract

miRNAs Regulate miRNAs: Coordinated Transcriptional and Post-Transcriptional Regulation

Cited by 34 publications

References 29 publications

A Unique MicroRNA Signature Associated With Plaque Instability in Humans

A Unique MicroRNA Signature Associated With Plaque Instability in Humans

Emerging Role of MicroRNAs in Cardiovascular Biology

Airway Smooth Muscle Hyperproliferation Is Regulated by MicroRNA-221 in Severe Asthma

Contact Info

Product

Resources

About