miRNAs: Tiny super-soldiers shaping the life of rice plants for facing “stress”-ful times

Das, Binayak; Sen, Anuska; Roy, Sanket; Banerjee, Oindrilla; Bhattacharya, Surajit

doi:10.1016/j.plgene.2021.100281

Cited by 3 publications

(1 citation statement)

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“…They are widely presented across eukaryotes, where most are negative regulators of gene expression [ 22 , 23 ]. They mainly regulate the expression of plant genes at the post-transcriptional level by mediating cleavage of mRNA target molecules or reducing the translation of target molecules, thereby regulating morphogenesis, growth, development, plant organ hormone secretion, plant organ signal transduction, and the ability to respond to stressors in the environment [ 24 , 25 , 26 ]. Recently, a growing body of studies have found that small RNAs were involved in regulating auxin and ethylene signal transduction.…”

Section: Introductionmentioning

confidence: 99%

Small RNAs, Degradome, and Transcriptome Sequencing Provide Insights into Papaya Fruit Ripening Regulated by 1-MCP

Cai

Hao

et al. 2021

Foods

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As an inhibitor of ethylene receptors, 1-methylcyclopropene (1-MCP) can delay the ripening of papaya. However, improper 1-MCP treatment will cause a rubbery texture in papaya. Understanding of the underlying mechanism is still lacking. In the present work, a comparative sRNA analysis was conducted after different 1-MCP treatments and identified a total of 213 miRNAs, of which 44 were known miRNAs and 169 were novel miRNAs in papaya. Comprehensive functional enrichment analysis indicated that plant hormone signal pathways play an important role in fruit ripening. Through the comparative analysis of sRNAs and transcriptome sequencing, a total of 11 miRNAs and 12 target genes were associated with the ethylene and auxin signaling pathways. A total of 1741 target genes of miRNAs were identified by degradome sequencing, and nine miRNAs and eight miRNAs were differentially expressed under the ethylene and auxin signaling pathways, respectively. The network regulation diagram of miRNAs and target genes during fruit ripening was drawn. The expression of 11 miRNAs and 12 target genes was verified by RT-qPCR. The target gene verification showed that cpa-miR390a and cpa-miR396 target CpARF19-like and CpERF RAP2-12-like, respectively, affecting the ethylene and auxin signaling pathways and, therefore, papaya ripening.

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