miRNome of inflammatory breast cancer

Abstract

BackgroundInflammatory breast cancer (IBC) is an extremely malignant form of breast cancer which can be easily misdiagnosed. Conclusive prognostic IBC molecular biomarkers which are also providing the perspectives for targeted therapy are lacking so far. The aim of this study was to reveal the IBC-specific miRNA expression profile and to evaluate its association with clinicopathological parameters.MethodsmiRNA expression profiles of 13 IBC and 17 non-IBC patients were characterized using comprehensive Affymetrix GeneChip miRNA 3.0 microarray platform. Bioinformatic analysis was used to reveal IBC-specific miRNAs, deregulated pathways and potential miRNA targets.Results31 differentially expressed miRNAs characterize IBC and mRNAs regulated by them and their associated pathways can functionally be attributed to IBC progression. In addition, a minimal predictive set of 4 miRNAs characteristic for the IBC phenotype and associated with the TP53 mutational status in breast cancer patients was identified.ConclusionsWe have characterized the complete miRNome of inflammatory breast cancer and found differentially expressed miRNAs which reliably classify the patients to IBC and non-IBC groups. We found that the mRNAs and pathways likely regulated by these miRNAs are highly relevant to cancer progression. Furthermore a minimal IBC-related predictive set of 4 miRNAs associated with the TP53 mutational status and survival for breast cancer patients was identified.Electronic supplementary materialThe online version of this article (doi:10.1186/1756-0500-7-871) contains supplementary material, which is available to authorized users.

MiR-548-3p functions as an anti-oncogenic regulator in breast cancer

“…However, miR-548 in breast cancer have not been fully elucidated. miR-548h was reported that it was lower in inflammatory breast cancer [9]. Consistant with this result, miR-548-3p, as a remember of miR-548 family, we found its expression was also down-regulated in clinical specimens and in breast cancer cells.…”

Detection of Potential Metastatic Prostate Cancer Circulating Biomarkers by Comparison of miRNA Profiles in DU145 Cells and Culture Medium

“…In 2, 3, 4, and 5 days of culturing, culture medium from one flask cultured simultaneously was collected and the cells were homogenized in QIAzol buffer (Qiagen). Total RNA was isolated from conditioned culture medium using miRNeasy Serum/Plasma Kit (Qiagen) with Ambion GlycoBlue Coprecipitant (15 mg/ml; Life Technologies) and from the cells using miRNeasy Mini Kit (Qiagen) as described previously [7,13].…”

A cross-cancer metastasis signature in the microRNA-mRNA axis of paired tissue samples

“…Meanwhile, induction of miR-1296 was found to induce apoptosis in triple negative breast cancer [28]. Although the other miRNAs presented in Figure 5 have not yet been associated with metastasis per se, several have been linked to cancer more generally, including miR-423 which is found to promote tumour progression in laryngeal cancer [15], and miR-101-2 and miR-4687 which are found to be associated with gastric cancer [31] and breast cancer [25] respectively. Confirmatory studies are needed to determine whether these miRNAs promote oncogenesis through the regulation of genes predicted by our random forest model.…”