Miscanthus accessions distinctively accumulate cadmium for largely enhanced biomass enzymatic saccharification by increasing hemicellulose and pectin and reducing cellulose CrI and DP

Cheng, Shaozhe; Yu, Hua; Hu, Meng; Wu, Yue; Cheng, Liangliang; Cai, Qiuming; Tu, Yuanyuan; Xia, Tao; Peng, Liangcai

doi:10.1016/j.biortech.2018.04.031

Cited by 42 publications

(12 citation statements)

References 44 publications

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“…Recent studies have explored the positive effect of Cd on saccharification efficiency [25], which may be related to the structure of the cell wall. Cd elements were semi-quantitatively determined, coupling with Si and Al as the reference elements (Table 2).…”

Section: Optimum Enzymatic Hydrolysis Ability Of Tetraploid Strawmentioning

confidence: 99%

Profiling of Chemical and Structural Composition of Lignocellulosic Biomasses in Tetraploid Rice Straw

Chen

et al. 2020

Polymers

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The improvement of the saccharification of rice straw is one of the strategies to reduce the sophisticated pretreatment that results in high cost and is unfriendly to the environment. We explored the cell wall features in tetraploid rice and highlighted the enhanced saccharification of tetraploid with large biomass. Results showed that lignin content and S/G ratio reduced to 17.09% and 0.37, respectively, in tetraploid straw by the determination of the pyGC-MS method. After the pretreatment, the cellulose crystallinity index decreased from 63.22% to 57.65% in tetraploid straw, which is lower than that of pretreated diploid straw. Surface topological analysis of SEM images indicated that tetraploid straw was more susceptible to the pretreatment. Tetraploid straw showed a strong advantage in the process of enzymatic hydrolysis. The enzyme efficiency reached the highest value of 77.60%, and the rate of enzyme reaction was improved to make the reaction saturated earlier than conventional rice. We concluded that the high saccharification has resulted from the alteration of lignin and cellulose in tetraploid rice. Our research provides an improved green feedstock for bioenergy, and the tetraploid rice straw shows the potential utilization value in bioethanol production.

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Section: Optimum Enzymatic Hydrolysis Ability Of Tetraploid Strawmentioning

confidence: 99%

Profiling of Chemical and Structural Composition of Lignocellulosic Biomasses in Tetraploid Rice Straw

Chen

et al. 2020

Polymers

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“…Cellulose consists of β-1, 4-glucan chains that form microfibrils with crystalline and amorphous regions. Cellulose crystalline index (CrI) and degree of polymerization (DP) are well demonstrated as the key negative factors accounting for biomass enzymatic digestibility [9][10][11]. In contrast, the arabinose (Ara) substitution degree of hemicelluloses in grasses has positive influence on biomass enzymatic saccharification by reducing the cellulose crystallinity [11,12].…”

Section: Introductionmentioning

confidence: 99%

Brassinosteroid overproduction improves lignocellulose quantity and quality to maximize bioethanol yield under green-like biomass process in transgenic poplar

Fan

Qin

et al. 2020

Biotechnol Biofuels

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Background: As a leading biomass feedstock, poplar plants provide enormous lignocellulose resource convertible for biofuels and bio-chemicals. However, lignocellulose recalcitrance particularly in wood plants, basically causes a costly bioethanol production unacceptable for commercial marketing with potential secondary pollution to the environment. Therefore, it becomes important to reduce lignocellulose recalcitrance by genetic modification of plant cell walls, and meanwhile to establish advanced biomass process technology in woody plants. Brassinosteroids, plantspecific steroid hormones, are considered to participate in plant growth and development for biomass production, but little has been reported about brassinosteroids roles in plant cell wall assembly and modification. In this study, we generated transgenic poplar plant that overexpressed DEETIOLATED2 gene for brassinosteroids overproduction. We then detected cell wall feature alteration and examined biomass enzymatic saccharification for bioethanol production under various chemical pretreatments.Results: Compared with wild type, the PtoDET2 overexpressed transgenic plants contained much higher brassinosteroids levels. The transgenic poplar also exhibited significantly enhanced plant growth rate and biomass yield by increasing xylem development and cell wall polymer deposition. Meanwhile, the transgenic plants showed significantly improved lignocellulose features such as reduced cellulose crystalline index and degree of polymerization values and decreased hemicellulose xylose/arabinose ratio for raised biomass porosity and accessibility, which led to integrated enhancement on biomass enzymatic saccharification and bioethanol yield under various chemical pretreatments. In contrast, the CRISPR/Cas9-generated mutation of PtoDET2 showed significantly lower brassinosteroids level for reduced biomass saccharification and bioethanol yield, compared to the wild type. Notably, the optimal green-like pretreatment could even achieve the highest bioethanol yield by effective lignin extraction in the transgenic plant. Hence, this study proposed a mechanistic model elucidating how brassinosteroid regulates cell © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article' s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article' Full list of author information is available at the end of the article wall modification for reduced lignocellulose recalcitrance and increased biomass porosity and accessibility for high bioethanol production. Conclusions:This study has demonstrated a powerful strategy to enhance cell...

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“…Because of its complex structure of combined cellulose (43.4%), hemicellulose (23.8%), and lignin (23.5%), biorefining MCT requires pretreatment processes such as alkaline, inorganic salt, or hydrothermal pretreatments [14–16]. In particular, HPAC and AASC pretreatments are widely used to delignify lignocellulosic biomass to increase the enzymatic conversion yield [17–19].…”

Section: Resultsmentioning

confidence: 99%

Advanced strategy to produce insecticidal destruxins from lignocellulosic biomass Miscanthus

Kim¹,

Choi²,

Lee³

et al. 2019

Biotechnol Biofuels

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Background Biorefineries are widely recognized as the most feasible solution to the problem of achieving environmental sustainability along with economic growth. Furthermore, pine wilt disease has caused severe environmental and economic damage worldwide to date. Herein, a highly efficient, advanced process for producing destruxins (DTXs) from Miscanthus (MCT) is reported, along with an application strategy. Results The acetic acid–sodium chlorite pretreatment of MCT (AASC-MCT) is found to improve the monosaccharide production. Through biocatalytic conversion processes (simultaneous saccharification and cultivation), Metarhizium anisopliae JEF-279 can efficiently produce DTXs from 1% (w/v) AASC-MCT, i.e., DTX E (334.8 mg/L), A (288.8 mg/L), and B (48.6 mg/L). Monochamus alternatus (MA, Japanese pine sawyer) is known to act as a mediator transferring Bursaphelenchus xylophilus to pinewood. As B. xylophilus is associated with the occurrence of pine wilt disease, biological control of MA is a major strategy or controlling this disease. In this study, upon the application of a mixture of DTXs and protease-containing culture filtrate (PCF), complete mortality of MA is observed after a 5-day incubation. The MA immune system response is believed to cause an overexpression of actin and tropomyosin as a defense mechanism against the flaccid paralysis induced by the DTXs and PCF treatment. Conclusions These results suggest that MCT can be used as a major feedstock in the biorefinery industry and that DTXs can be applied as an insecticide for biological control of pine wilt disease via MA termination. Electronic supplementary material The online version of this article (10.1186/s13068-019-1530-8) contains supplementary material, which is available to authorized users.

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Miscanthus accessions distinctively accumulate cadmium for largely enhanced biomass enzymatic saccharification by increasing hemicellulose and pectin and reducing cellulose CrI and DP

Cited by 42 publications

References 44 publications

Profiling of Chemical and Structural Composition of Lignocellulosic Biomasses in Tetraploid Rice Straw

Profiling of Chemical and Structural Composition of Lignocellulosic Biomasses in Tetraploid Rice Straw

Brassinosteroid overproduction improves lignocellulose quantity and quality to maximize bioethanol yield under green-like biomass process in transgenic poplar

Advanced strategy to produce insecticidal destruxins from lignocellulosic biomass Miscanthus

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