Miscibility of Egg Yolk Lecithin with Palmitic Acid and Hexadecanol at the Air-Water Interface

Nakahara, Hiromichi; Shibata, Osamu

doi:10.5650/jos.62.471

Cited by 2 publications

(3 citation statements)

References 30 publications

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“…The new features observed due to liposome interaction/incorporation into the octadecanol layer are not the partial incorporation of liposomes anticipated but are more like the existence of a new phase of lipid and octadecanol which is thicker than the original adsorbed octadecanol layer. The solubility and phase behavior of 1-alkanols in lipid monolayers or bilayers have been studied. − For a DMPC lipid monolayer with added 1-dodecanol, a decrease in the molar volume of the alcohol was observed when comparing the pure state of the alkanols to the state when in the membrane. The 1-alkanol resides in the tail region of the membrane, with the alcohol group interacting with the PC headgroup.…”

Section: Resultsmentioning

confidence: 99%

“…Even though these are shorter chains than the DOPC studied here, the fluidity is similar. The interaction of hexadecanol with eggPC (a mixture of lipids with either one or two unsaturated alkyl tails; in DOPC, both tails have one double bond) 52 showed that the compounds were partially miscible and can induce a phase change in eggPC from an expanded (LE) to a condensed (LC) phase. The creation of these higher (thicker) regions after the incorporation of DOPC into the octadecanol adsorbed layer may be explained by the formation of a mixed layer in these regions in which the DOPC is partially miscible.…”

Section: ■ Methods and Materialsmentioning

confidence: 99%

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Potential Controls the Interaction of Liposomes with Octadecanol-Modified Au Electrodes: An in Situ AFM Study

Musgrove

Bizzotto

2015

Langmuir

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The formation of supported lipid bilayers using liposomes requires interaction with the solid surface, rupture of the liposome, and spreading to cover the surface with a lipid bilayer. This can result in a less-than-uniform coating of the solid surface. Presented is a method that uses the electrochemical poration of an adsorbed lipid-like layer on a Au electrode to control the interaction of 100 nm DOPC liposomes. An octadecanol-coated Au-on-mica surface was imaged using tapping-mode AFM during the application of potential in the presence or absence of liposomes. When the substrate potential was made negative enough, defects formed in the adsorbed layer and new taller features were observed. More features were observed and existing features increased in size with time spent at this negative poration potential. The new features were 1.8-2.0 nm higher than the octadecanol-coated gold surface, half the thickness of a DOPC bilayer. These features were not observed in the absence of liposomes when undergoing the same potential perturbation. In the presence of liposomes, the application of a poration potential was needed to initiate the formation of these taller features. Once the applied potential was removed, the features stopped growing and no new regions were observed. The size of these new regions was consistent with the footprint of a flattened 100 nm liposome. It is speculated that the DOPC liposomes were able to interact with the defects and became soluble in the octadecanol, creating a taller region that was limited in size to the liposome that adsorbed and became incorporated. This AFM study confirms previous in situ fluorescence measurements of the same system and illustrates the use of a potential perturbation to control the formation of these regions of increased DOPC content.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Methods and Materialsmentioning

confidence: 99%

Potential Controls the Interaction of Liposomes with Octadecanol-Modified Au Electrodes: An in Situ AFM Study

Musgrove

Bizzotto

2015

Langmuir

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“…Recently, it was reported that a hydrogenated soy lecithin/soy lecithin/PA/Hel 13-5 mixture exhibited surfactant activity comparable to that of Surfacten ® 27, 28 ; this suggests that artificial surfactants that do not contain synthetic DPPC and PG are possible. In a previous study 19 , we considered egg yolk phosphatidylcholine eggPC extracted and purified from relatively inexpensive egg yolk as an alternative to DPPC. The fatty-acid moieties in eggPC comprised double hydrocarbon chains composed of both saturated and unsaturated acyl chains: DPPC 2C16:0 was a large component.…”

Section: Introductionmentioning

confidence: 99%

Interfacial Behavior of Pulmonary Surfactant Preparations Containing Egg Yolk Lecithin

Nakahara

Shibata

2014

J. Oleo Sci.

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Miscibility of Egg Yolk Lecithin with Palmitic Acid and Hexadecanol at the Air-Water Interface

Cited by 2 publications

References 30 publications

Potential Controls the Interaction of Liposomes with Octadecanol-Modified Au Electrodes: An in Situ AFM Study

Potential Controls the Interaction of Liposomes with Octadecanol-Modified Au Electrodes: An in Situ AFM Study

Interfacial Behavior of Pulmonary Surfactant Preparations Containing Egg Yolk Lecithin

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