Mise au point : les uropathogènes émergents

Brun, Cécile; Robert, Sylvie; Bruyère, F.; Lanotte, Philippe

doi:10.1016/j.purol.2015.01.021

Cited by 5 publications

(5 citation statements)

References 36 publications

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“…The use of MALDI-TOF MS for bacterial species identification has led to the emergence of several microorganisms once thought to be uncommon human pathogens (24)(25)(26)(27). Phenotypic characteristics of SIG, including the presence of beta-hemolysis on blood agar and a positive coagulase reaction, may lead to the mischaracterization of SIG as S. aureus in human clinical isolates (7,8).…”

Section: Discussionmentioning

confidence: 99%

Epidemiology, Clinical Characteristics, and Antimicrobial Susceptibility Profiles of Human Clinical Isolates of Staphylococcus intermedius Group

2018

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The veterinary pathogens in the group (SIG) are increasingly recognized as causes of human infection. Shared features between SIG and may result in the misidentification of SIG in human clinical cultures. This study examined the clinical and microbiological characteristics of isolates recovered at a tertiary-care academic medical center. From 2013 to 2015, 81 SIG isolates were recovered from 62 patients. Patients were commonly ≥50 years old, diabetic, and/or immunocompromised. Documentation of dog exposure in the electronic medical record was not common. Of the 81 SIG isolates, common sites of isolation included 37 (46%) isolates from wound cultures and 17 (21%) isolates from respiratory specimens. Although less common, 10 (12%) bloodstream infections were documented in 7 unique patients. The majority of SIG (65%) isolates were obtained from polymicrobial cultures. In comparison to isolates from the same time period, significant differences were noted in proportion of SIG isolates that were susceptible to doxycycline (74% versus 97%, respectively; < 0.001), trimethoprim-sulfamethoxazole (65% versus 97%, respectively; < 0.001), and ciprofloxacin (78% versus 59%, respectively; < 0.01). Methicillin resistance (MR) was detected in 12 (15%) of 81 SIG isolates. All MR isolates detected by an oxacillin disk diffusion test would have been misclassified as methicillin susceptible using a cefoxitin disk diffusion test. Thus, SIG is recovered from human clinical specimens, and distinction of SIG from is critical for the accurate characterization of MR status in these isolates.

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Section: Discussionmentioning

confidence: 99%

Epidemiology, Clinical Characteristics, and Antimicrobial Susceptibility Profiles of Human Clinical Isolates of Staphylococcus intermedius Group

2018

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“…CAN or CAP) should be seeded and incubated under an anaerobic atmosphere. Few recommendations and clarifications have been made to inform urologists and biologists of how and when to investigate the presence of A. schaalii [11,[48][49][50]. Traditional phenotypic tests, such as the API system, are not reliable for the identification of A. schaalii because this species is not included in their database.…”

Section: Methods Of Culture and Identificationmentioning

confidence: 99%

“…Initially, 16S rRNA gene sequencing of colonies, or alternatively specific real-time PCR assays, were successfully used for the identification of this bacterium [7,11,12,15,[17][18][19][20]22,23,25,27,30,34,44,51]. Today, MALDI-TOF using Microflex LT with the Biotyper 2.3 database (Bruker Daltonics, Bremen, Germany) of colonies is probably the most accurate and rapid method for the routine identification of A. schaalii [35,[48][49][50]. Indeed, its utilization is faster and easier than 16S rRNA gene sequencing, and several studies have shown its efficiency [14,28,35,50].…”

Section: Methods Of Culture and Identificationmentioning

confidence: 99%

“…Interestingly, among the two most widely used MALDI-TOF system for the routine identification of bacteria, only the Microflex Biotyper (Bruker Daltonics) is able to provide good identification of A. schaalii. Indeed, the Biotyper database contain three spectra corresponding to A. schaalii (type strain DSM 15541 T and two strains from Bruker's personal bank), while the VitekMS (bioMérieux) misidentified A. schaalii strains [15] as G. vaginalis or A. meyeri [48]. Finally, Farfour et al [52] have demonstrated that the Andromas MALDI-TOF system is able to provide reliable identification of A. schaalii.…”

Section: Methods Of Culture and Identificationmentioning

confidence: 99%

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Actinotignum schaalii (formerly Actinobaculum schaalii ): a newly recognized pathogen—review of the literature

Lotte

Ruimy

2016

Clinical Microbiology and Infection

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The genus Actinotignum contains three species, Actinotignum schaalii (formerly Actinobaculum schaalii), Actinotignum urinale and Actinotignum sanguinis. A. schaalii is the species most frequently involved in human infections, with 172 cases, mostly urinary tract infections (UTIs), reported so far. Invasive infections have also been described. This facultative anaerobic Gram-positive rod is part of the urinary microbiota of healthy patients. It is responsible for UTIs, particularly in elderly men and young children. A. schaalii is an underestimated cause of UTIs because of its fastidious growth on usual media and difficulties associated with its identification using phenotypic methods. Indeed, this slow-growth bacterium requires blood-enriched media and an incubation time of 48 hours under anaerobic or 5% CO2 atmosphere. Furthermore, only matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) or molecular-based methods allow the accurate identification of this bacteria. MALDI-TOF using Microflex LT with the Biotyper database (Bruker Daltonics, Bremen, Germany) is the most reliable technology for the routine identification of A. schaalii. The identification of this uropathogen is all the more important because it is resistant to trimethoprim/sulfamethoxazole and second-generation quinolones that are widely used in the treatment of UTIs. Antimicrobial therapy using β-lactams prolonged for up to 2 weeks is the most efficient treatment and should be recommended. Microbiologists should assess the presence of A. schaalii in urine using appropriate culture and identification methods in the case of a direct examination that is positive for small coccoid rods, a negative nitrite urinary stick associated with leukocyturia, treatment failure with trimethoprim/sulfamethoxazole or fluoroquinolones, or undocumented, repeated UTIs.

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“…As previously described [10] , we confirmed the identification performance by Microflex MALDI-TOF (Bruker) for A. schaalii with the three strains also identified by sequencing. However, it was reported that the MALDI-TOF Vitek (bioMérieux) does not currently allow the identification of A. schaalii [11] , [12] .…”

Section: Discussionmentioning

confidence: 99%

Actinobaculum schaalii: A truly emerging pathogen?

Prigent¹,

Perillaud²,

Amara³

et al. 2016

New Microbes and New Infections

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Actinobaculum schaalii is a Gram-positive facultative anaerobe bacillus. It is a commensal organism of the genitourinary tract. Its morphology is nonspecific. Aerobic culture is tedious, and identification techniques have long been inadequate. Thus, A. schaalii has often been considered as a nonpathogen bacterium or a contaminant. Its pathogenicity is now well described in urinary tract infections, and infections in other sites have been reported. This pathogen is considered as an emerging one following the growing use of mass spectrometry identification. In this context, the aim of our study was to evaluate the number of isolations of A. schaalii before and after the introduction of mass spectrometry in our hospital and to study the clinical circumstances in which isolates were found.

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Mise au point : les uropathogènes émergents

Cited by 5 publications

References 36 publications

Epidemiology, Clinical Characteristics, and Antimicrobial Susceptibility Profiles of Human Clinical Isolates of Staphylococcus intermedius Group

Epidemiology, Clinical Characteristics, and Antimicrobial Susceptibility Profiles of Human Clinical Isolates of Staphylococcus intermedius Group

Actinotignum schaalii (formerly Actinobaculum schaalii ): a newly recognized pathogen—review of the literature

Actinobaculum schaalii: A truly emerging pathogen?

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