Misidentification of Aspergillus nomius and Aspergillus tamarii as Aspergillus flavus: Characterization by Internal Transcribed Spacer, β-Tubulin, and Calmodulin Gene Sequencing, Metabolic Fingerprinting, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Tam, Eric; Chen, Jonathan Hon-Kwan; Lau, Eunice C. L.; Ngan, Antonio H. Y.; Fung, Kitty S. C.; Lee, Kim-Chung; Lam, Chi Fung; Yuen, Kwok‐Yung; Lau, Susanna K. P.; Woo, Patrick C. Y.

doi:10.1128/jcm.03258-13

Cited by 85 publications

(82 citation statements)

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“…Moreover, it often takes 2 to 6 weeks before culture is positive and even longer for definitive species identification. While newer diagnostic modalities, such as adenosine deaminase levels in pleural fluid, lipoarabinomannan levels in urine, PCR, and Xpert MTB/RIF assays, have been developed (6-12), there are still limitations in terms of their sensitivity and/or specificity.Metabolomics is an emerging platform for studies of infectious diseases or pathogens (13)(14)(15)(16)(17)(18)(19). For TB, the technique has been applied on cultured isolates for differentiation from other Mycobacterium species and studies on the biology and virulence of tuberculosis (14,15,(20)(21)(22).…”

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Metabolomic Profiling of Plasma from Patients with Tuberculosis by Use of Untargeted Mass Spectrometry Reveals Novel Biomarkers for Diagnosis

et al. 2015

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h Although tuberculosis (TB) is a reemerging disease that affects people in developing countries and immunocompromised populations in developed countries, the current diagnostic methods are far from optimal. Metabolomics is increasingly being used for studies on infectious diseases. We performed metabolome profiling of plasma samples to identify potential biomarkers for diagnosing TB. We compared the plasma metabolome profiles of TB patients (n ‫؍‬ 46) with those of community-acquired pneumonia (CAP) patients (n ‫؍‬ 30) and controls without active infection (n ‫؍‬ 30) using ultrahigh-performance liquid chromatographyelectrospray ionization-quadrupole time of flight mass spectrometry (UHPLC-ESI-QTOFMS). Using multivariate and univariate analyses, four metabolites, 12R-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid [12(R)-HETE], ceramide (d18:1/16:0), cholesterol sulfate, and 4␣-formyl-4␤-methyl-5␣-cholesta-8-en-3␤-ol, were identified and found to have significantly higher levels in TB patients than those in CAP patients and controls. In a comparison of TB patients and controls, the four metabolites demonstrated area under the receiver operating characteristic curve ( T uberculosis (TB) is a disease caused by the bacterium Mycobacterium tuberculosis. Although it is a well-known disease that has been around for much of human history, there are still millions of new TB cases occurring per year worldwide, and TB remains a leading cause of deaths worldwide, especially in developing countries. Since the 1980s, TB has reemerged as a result of the AIDS epidemic and increasing use of immunosuppressants. In recent years, a higher incidence of extrapulmonary disease in immunocompromised hosts and the emergence of multidrug-resistant strains have further complicated diagnosis and treatment (1-3). Despite the medical importance of TB, diagnosis is still associated with many unresolved problems. The traditional gold standard methods are smear and culture for acid-fast bacilli from clinical specimens. Although culture offers higher sensitivity and specificity than those of smears, it is not useful for culture-negative cases, especially in early, disseminated, or extrapulmonary disease (4, 5). Moreover, it often takes 2 to 6 weeks before culture is positive and even longer for definitive species identification. While newer diagnostic modalities, such as adenosine deaminase levels in pleural fluid, lipoarabinomannan levels in urine, PCR, and Xpert MTB/RIF assays, have been developed (6-12), there are still limitations in terms of their sensitivity and/or specificity.Metabolomics is an emerging platform for studies of infectious diseases or pathogens (13)(14)(15)(16)(17)(18)(19). For TB, the technique has been applied on cultured isolates for differentiation from other Mycobacterium species and studies on the biology and virulence of tuberculosis (14,15,(20)(21)(22). For example, lipidomics studies have revealed novel metabolites potentially associated with growth and virulence of M. tuberculosis (23,24). We also recently identified ...

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Metabolomic Profiling of Plasma from Patients with Tuberculosis by Use of Untargeted Mass Spectrometry Reveals Novel Biomarkers for Diagnosis

et al. 2015

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“…Strains HKU33 T and HKU34 were resistant to ciprofloxacin and trimethoprim-sulphamethoxazole but sensitive to penicillin, amoxicillin-clavulanic acid, imipenem, ceftriaxone, cefotaxime, vancomycin, levofloxacin, chloramphenicol and metronidazole (Table S2). The whole-cell protein content was analysed by matrix-assisted laser desorption/ionization time-of-flight MS as described previously (Tam et al, 2014) with modifications, using bacterial cells cultured on Columbia agar with 5 % defibrinated sheep blood, and with the bacterial extraction incubated in formic acid for 1-2 min, and E. coli ATCC 8739 was used as a control. Hierarchical cluster analysis of mass spectra of whole-cell protein contents using LaunchPad 2.8 (ShimadzuBiotech) showed that strains HKU33 T and HKU34 were related more closely to each other than to Streptobacillus moniliformis CCUG 13453 T , Sneathia sanguinegens CCUG 41628 T or 'L.…”

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Streptobacillus hongkongensis sp. nov., isolated from patients with quinsy and septic arthritis, and emended descriptions of the genus Streptobacillus and Streptobacillus moniliformis

Woo

Tsang

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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Two bacterial strains, HKU33 T and HKU34, were isolated in Hong Kong from the pus aspirated from the right peritonsillar abscess of a patient with quinsy and the left elbow joint fluid of another patient with tophaceous gout and left elbow septic arthritis, respectively. The bacteria were Gramstain-negative, non-motile, non-spore-forming, non-haemolytic pleomorphic bacilli. They grew best on Columbia agar with 5 % defibrinated sheep blood in an anaerobic environment or aerobic environment with 5 % CO 2 . They also grew on chocolate agar but not on MacConkey agar. They were catalase-and cytochrome oxidase-negative. They showed a unique profile of enzyme activities distinguishable from their closely related species. Phylogenetic analysis of the complete 16S rRNA gene, and partial groEL, gyrB and recA gene sequences showed the two isolates formed a distinct branch within the family Leptotrichiaceae, being related most closely to Streptobacillus moniliformis. Hierarchical cluster analysis of mass spectra of whole-cell protein contents showed that strains HKU33 T and HKU34 were closely related to each other, but were distinct from Streptobacillus moniliformis, Sneathia sanguinegens and 'Leptotrichia amnionii'. The DNA G+C content of strain HKU33 T was 26.0±2.1 mol% (mean±SD; n53). DNA-DNA hybridization demonstrated ¡45.02 % DNA relatedness between the two isolates and Streptobacillus moniliformis CCUG 13453 T . A novel species, Streptobacillus hongkongensis sp. nov., is proposed to accommodate strains HKU33 T and HKU34, with HKU33 T (5JCM 18691 T 5NCTC 13659 T 5DSM 26322 T ) designated the type strain. Emended descriptions of the genus Streptobacillus and Streptobacillus moniliformis are also given.

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“…It was also identified in house dust samples from Mexico [43]. This species has also been found to be able to cause human infections including onychomycosis in Italy [44], keratitis in India [22], invasive aspergillosis, cavitary and fibrosing pulmonary and pleural aspergillosis in Hong Kong [39] and breakthrough pneumonia in Italy [4]. Besides, it was also identified as the causative agent of stonebrood disease in honeybees in Great Britain [13].…”

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Identification ofAspergillusspecies in Central Europe able to produce G-type aflatoxins

Baranyi¹,

Despot²,

Palágyi³

et al. 2015

Acta Biologica Hungarica

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The occurrence of potential aflatoxin producing fungi was examined in various agricultural products and indoor air in Central European countries including Hungary, Serbia and Croatia. For species identification, both morphological and sequence based methods were applied. Aspergillus flavus was detected in several samples including maize, cheese, nuts, spices and indoor air, and several isolates were able to produce aflatoxins. Besides, three other species of Aspergillus section Flavi, A. nomius, A. pseudonomius and A. parasiticus were also isolated from cheese, maize and indoor air, respectively. This is the first report on the occurrence of A. nomius and A. pseudonomius in Central Europe. All A. nomius, A. pseudonomius and A. parasiticus isolates were able to produce aflatoxins B 1 , B 2 , G 1 and G 2 . The A. nomius isolate came from cheese produced very high amounts of aflatoxins (above 1 mg ml -1 ). All A. nomius, A. pseudonomius and A. parasiticus isolates produced much higher amounts of aflatoxin G 1 then aflatoxin B 1 . Further studies are in progress to examine the occurrence of producers of these highly carcinogenic mycotoxins in agricultural products and indoor air in Central Europe.

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Misidentification of Aspergillus nomius and Aspergillus tamarii as Aspergillus flavus: Characterization by Internal Transcribed Spacer, β-Tubulin, and Calmodulin Gene Sequencing, Metabolic Fingerprinting, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Cited by 85 publications

References 32 publications

Metabolomic Profiling of Plasma from Patients with Tuberculosis by Use of Untargeted Mass Spectrometry Reveals Novel Biomarkers for Diagnosis

Metabolomic Profiling of Plasma from Patients with Tuberculosis by Use of Untargeted Mass Spectrometry Reveals Novel Biomarkers for Diagnosis

Streptobacillus hongkongensis sp. nov., isolated from patients with quinsy and septic arthritis, and emended descriptions of the genus Streptobacillus and Streptobacillus moniliformis

Identification ofAspergillusspecies in Central Europe able to produce G-type aflatoxins

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