Misidentification of Listeria monocytogenes by the Vitek 2 System

Lappe, Niall De; Lee, Ciara; O’Connor, Jacqueline; Cormican, Martin

doi:10.1128/jcm.01725-14

Cited by 13 publications

(12 citation statements)

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“…; De Lappe et al . ; Day and Basavanna ). Molecular techniques, such as real‐time PCR, have been employed to detect L. monocytogenes in various fruits including apples, grapes, cantaloupe, mango, strawberries and oranges which achieve high sensitivities but require multiple days of enrichment (Shearer et al .…”

Section: Discussionmentioning

confidence: 96%

“…Previous immunological techniques used for L. monocytogenes detection in foods (ELISA, fibre-optic immune-sensors, magnetic bead-antibody conjugates) have been limited due to low sensitivity, misidentifications, prolonged detection times or susceptibility to false-positive results (Geng et al 2004;Johnson et al 2013;De Lappe et al 2014;Day and Basavanna 2015). Molecular techniques, such as real-time PCR, have been employed to detect L. monocytogenes in various fruits including apples, grapes, cantaloupe, mango, strawberries and oranges which achieve high sensitivities but require multiple days of enrichment (Shearer et al 2001;Badosa et al 2009).…”

Section: Discussionmentioning

confidence: 99%

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Immuno‐detection and differentiation ofListeria monocytogenesandListeria ivanoviiin stone fruits

Day

Hammack

2019

J Appl Microbiol

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Aims The aim of this study was to develop a rapid detection and differentiation method for pathogenic Listeria species in stone fruits. Methods and Results We utilized activated charcoal enrichment media (ACM) to induce overexpression and hypersecretion of pathogenic Listeria virulence proteins which can subsequently be detected via immunoblot analysis. Plum and nectarine slices spiked with either L. monocytogenes or L. ivanovii were incubated in pre‐enrichment broth followed by enrichment in ACM. Secreted proteins were precipitated and subjected to SDS‐PAGE and immunoblot analysis using a combination of L. monocytogenes‐specific antibody (α‐listeriolysin O) and antibody specific for both L. monocytogenes and L. ivanovii (α‐Internalin C). As low as 1 CFU per gram of L. monocytogenes in plum and nectarine was detected, whereas a detection limit of 10 CFU per gram was achieved for L. ivanovii in each food tested following a 20‐h enrichment period. Nonpathogenic Listeria species and non‐Listeria bacterial pathogens tested were negative. Conclusions These results demonstrate the highly sensitive and specific nature of the detection method for pathogenic Listeria in stone fruits using activated charcoal enrichment as well as the capability to discriminate between L. monocytogenes and L. ivanovii. Significance and Impact of the Study This method is the first to identify and differentiate L. monocytogenes and L. ivanovii in select stone fruit enrichments within 24 h using immunological techniques. The rapidity and sensitivity of the method could aid in the reduction of exposure to the public in the event of an outbreak and expedite the administration of appropriate antibiotics to infected individuals.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Immuno‐detection and differentiation ofListeria monocytogenesandListeria ivanoviiin stone fruits

Day

Hammack

2019

J Appl Microbiol

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“…However, it was recently shown to be inaccurate in 1.4 % of cases seen due to atypical L. monocytogenes isolates which were negative for a phospholipase C reaction. According to the Vitek 2 method, these isolates were identifi ed as L. innocua but were later confi rmed as L. monocytogenes by PCR identifi cation methods (De Lappe et al 2014 ). Differentiation of L. monocytogenes as distinct from other Listeria species has also been seen to be possible through phage typing.…”

Section: Confi Rmation Of Isolates As L Monocytogenesmentioning

confidence: 99%

Listeria monocytogenes in the Food Processing Environment

Jordan

Leong

Ordóñez

2015

SpringerBriefs in Food, Health, and Nutrition

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Springer Briefs in Food, Health, and Nutrition present concise summaries of cutting edge research and practical applications across a wide range of topics related to the fi eld of food science, including its impact and relationship to health and nutrition.We are especially interested in how these areas impact or are related to health and nutrition.Featuring compact volumes of 50 to 125 pages, the series covers a range of content from professional to academic. Typical topics might include:• A timely report of state-of-the art analytical techniques • A bridge between new research results, as published in journal articles, and a contextual literature review • A snapshot of a hot or emerging topic • An in-depth case study • A presentation of core concepts that students must understand in order to make independent contributionsMore information about this series at

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“…may not be distinguished using conventional techniques such as selective and differential media and standard biochemical tests for differentiation of L. monocytogenes, L. innocua, L. ivanovii and L. seeligeri strains that show similar results to L. monocytogenes in these tests (10,11). It was found that 1.4% of L. monocytogenes isolates were misidentified as L. innocua using Vitek 2 (12). Therefore, Listeria spp.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, Listeria spp. confirmation must be performed by molecular methods such as polymerase chain reaction (PCR) and sequencing (12,13).…”

Section: Introductionmentioning

confidence: 99%

Detection and pathogenicity of Listeria monocytogenes in common carp (Cyprinus carpio) fish in Baghdad, Iraq

Al-Gburi¹

2020

IJVS

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A few reports are available for detection of L. monocytogenes in fish in Iraq, however, the current study was undertaken to investigate the potential role of Listeria spp. in common carp fish in Baghdad province, Iraq. A total of fresh thirty raw common carp (Cyprinus carpio) were purchased from fish sellers of various local markets in Baghdad city from (December 2017 to March 2018) The viscera was removed aseptically, the bacterial isolation and identification was conducted by a conventional culture method using Listeria selective media, biochemical tests and Vitek 2 for gram-positive. Pathogenicity of isolates was studied in vivo by inoculating mice with bacterium. Targeting virulence associated genes was used to detect the virulence and to confirm the L. monocytogenes isolates. The isolates were tested for antimicrobial susceptibility by disk diffusion method for 12 antibiotics. The results revealed that 6.66% of L. monocytogenes were identified from common carp fish viscera and the isolates were pathogenic in mice. L. monocytogenes virulence associated genes were detected in both isolates, while L. innocua virulence associated gene (Lin0372) was detected in one of the two isolates. The isolates were resistant to 7 out of 12 antibacterial drugs including tetracycline, ampicillin, methicillin, cefixime, oxacillin, cefotaxime and penicillin G. The results suggest that presence of L. monocytogenes in fish may have a serious role in public health hygienic in humans.

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Misidentification of Listeria monocytogenes by the Vitek 2 System

Cited by 13 publications

References 5 publications

Immuno‐detection and differentiation ofListeria monocytogenesandListeria ivanoviiin stone fruits

Immuno‐detection and differentiation ofListeria monocytogenesandListeria ivanoviiin stone fruits

Listeria monocytogenes in the Food Processing Environment

Detection and pathogenicity of Listeria monocytogenes in common carp (Cyprinus carpio) fish in Baghdad, Iraq

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