2022
DOI: 10.1016/j.foodcont.2021.108692
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Mislabeling assessment and species identification by PCR-RFLP of mussel-based products (Mytilus spp.) sold on the Italian market

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Cited by 6 publications
(5 citation statements)
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“…The only two substitutions encountered consisted of the replacement of M. galloprovincialis with M. chilensis. Similar substitutions are described in products imported from Chile and have been attributed to unintentional accidents related to the coexistence of the two species in fishing and aquaculture areas along Chilean coasts [21]. However, this observation does not apply to one of the two mislabeled products in the study (MB13), a canned marinated product, for which the clear North Atlantic (FAO 27) origin of the product was declared on the label and a fraudulent action is clearly hypothesized and collocable at the product processing stage.…”
Section: Mislabeling Rate and Products Originsupporting
confidence: 66%
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“…The only two substitutions encountered consisted of the replacement of M. galloprovincialis with M. chilensis. Similar substitutions are described in products imported from Chile and have been attributed to unintentional accidents related to the coexistence of the two species in fishing and aquaculture areas along Chilean coasts [21]. However, this observation does not apply to one of the two mislabeled products in the study (MB13), a canned marinated product, for which the clear North Atlantic (FAO 27) origin of the product was declared on the label and a fraudulent action is clearly hypothesized and collocable at the product processing stage.…”
Section: Mislabeling Rate and Products Originsupporting
confidence: 66%
“…Additionally and alternatively, PCR-restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), or single-stranded conformational polymorphism (SSCP) on both mitochondrial and nuclear targets have been applied as targeted methods to design genus or species-specific assays [19,20]. In this regard, the validity of an RFLP protocol on the PAP gene for species-specific discrimination of bivalve mollusks-mussels-belonging to the genus Mytilus sp., has been confirmed [21].…”
Section: Introductionmentioning
confidence: 77%
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“…Food traceability and authenticity in Mytilus mussels is challenging due to the natural hybridization observed in many areas where these species are cultured ( Michalek et al, 2016 ). In the case of M. chilensis and M. galloprovincialis the presence of hybrids has been highlighted in molecular identification studies conducted on cultured specimens in Arauco and Reloncaví gulfs ( Larraín et al, 2012 , Larraín et al, 2019 ) and on exported Chilean mussel products collected from retail ( Giusti et al, 2022 ). To overcome this challenge, new molecular techniques are necessary to separate hybrids from the parental taxa in an efficient a cost-effective way.…”
Section: Discussionmentioning
confidence: 99%
“…Interestingly, the PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method is based on the amplification of a DNA region and the subsequent digestion of the amplicon by restriction endonucleases that recognize restriction sites and cut the DNA sequence into fragments of different lengths [11,12]. The speciesspecific pattern of DNA fragments obtained has been widely used and validated for the genetic authentication of species in both fish and meat products using various nuclear and mitochondrial genes [13][14][15][16][17][18]. In particular, PCR-RFLP of the COI gene has been successfully used to identify tuna species in raw and cooked tuna products [13] and recently for the recognition and discrimination of 9 of 25 meat species [17].…”
Section: Introductionmentioning
confidence: 99%