Mismatch amplification mutation assay-polymerase chain reaction

Deekshit, Vijaya Kumar; Jazeela, Kadeeja; Chakraborty, Gunimala; Rohit, Anusha; Chakraborty, Anirban; Karunasagar, Indrani

doi:10.4103/ijmr.ijmr_2091_17

Cited by 7 publications

(4 citation statements)

References 20 publications

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“…The detection of all gyrA mutations which confer resistance is helpful in rapid molecular diagnosis of FQN resistance [ 261 ]. Mismatch amplification mutation assay-polymerase chain reaction (MAMA-PCR) technique may serve as a tool to identify the multiple point mutations in the FQN resistance in Gram-negative bacteria [ 262 ].…”

Section: Antimicrobial Resistance To the Newer Fqnsmentioning

confidence: 99%

Structural Characterization of the Millennial Antibacterial (Fluoro)Quinolones—Shaping the Fifth Generation

et al. 2021

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The evolution of the class of antibacterial quinolones includes the introduction in therapy of highly successful compounds. Although many representatives were withdrawn due to severe adverse reactions, a few representatives have proven their therapeutical value over time. The classification of antibacterial quinolones into generations is a valuable tool for physicians, pharmacists, and researchers. In addition, the transition from one generation to another has brought new representatives with improved properties. In the last two decades, several representatives of antibacterial quinolones received approval for therapy. This review sets out to chronologically outline the group of approved antibacterial quinolones since 2000. Special attention is given to eight representatives: besifloxacin, delafoxacin, finafloxacin, lascufloxacin, nadifloxacin and levonadifloxacin, nemonoxacin, and zabofloxacin. These compounds have been characterized regarding physicochemical properties, formulations, antibacterial activity spectrum and advantageous structural characteristics related to antibacterial efficiency. At present these new compounds (with the exception of nadifloxacin) are reported differently, most often in the fourth generation and less frequently in a new generation (the fifth). Although these new compounds’ mechanism does not contain essential new elements, the question of shaping a new generation (the fifth) arises, based on higher potency and broad spectrum of activity, including resistant bacterial strains. The functional groups that ensured the biological activity, good pharmacokinetic properties and a safety profile were highlighted. In addition, these new representatives have a low risk of determining bacterial resistance. Several positive aspects are added to the fourth fluoroquinolones generation, characteristics that can be the basis of the fifth generation. Antibacterial quinolones class continues to acquire new compounds with antibacterial potential, among other effects. Numerous derivatives, hybrids or conjugates are currently in various stages of research.

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Section: Antimicrobial Resistance To the Newer Fqnsmentioning

confidence: 99%

Structural Characterization of the Millennial Antibacterial (Fluoro)Quinolones—Shaping the Fifth Generation

et al. 2021

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“…Due to the lack of 3′-5′ exonucleolytic proofreading activity in Taq polymerase, the mismatch between the primer’s 3′ terminal base and the template will stop the PCR (Tindall 1988 ). Given this, MAMA PCR was well established and experimented in the late 1980s in the detection of point mutation of several disease conditions (Cui et al 2016 ; Deekshit et al 2019 ; Santhosh et al 2017 ). The MAMA PCR technique is also widely used for the detection of point mutations in the quinolone resistance determining regions (QRDRs) of fluoroquinolone-resistant bacterial pathogens (Deekshit et al 2019 ; Kakuta et al 2020 ; Ota et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…Given this, MAMA PCR was well established and experimented in the late 1980s in the detection of point mutation of several disease conditions (Cui et al 2016 ; Deekshit et al 2019 ; Santhosh et al 2017 ). The MAMA PCR technique is also widely used for the detection of point mutations in the quinolone resistance determining regions (QRDRs) of fluoroquinolone-resistant bacterial pathogens (Deekshit et al 2019 ; Kakuta et al 2020 ; Ota et al 2022 ). The basis of the technique is dependent on primer designing.…”

Section: Introductionmentioning

confidence: 99%

Rapid screening of point mutations by mismatch amplification mutation assay PCR

Zhang,

Liu,

Liu

et al. 2024

Appl Microbiol Biotechnol

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Metabolic engineering frequently makes use of point mutation and saturation mutation library creation. At present, sequencing is the only reliable and direct technique to detect point mutation and screen saturation mutation library. In this study, mismatch amplification mutation assay (MAMA) PCR was used to detect point mutation and screen saturation mutation library. In order to fine-tune the expression of odhA encoding 2-oxoglutarate dehydrogenase E1 component, a saturating mutant library of the RBS of odhA was created in Corynebacterium glutamicum P12 based on the CRISPR-Cas2a genome editing system, which increased the l-proline production by 81.3%. MAMA PCR was used to filter out 42% of the non-mutant transformants in the mutant library, which effectively reduced the workload of the subsequent fermentation test and the number of sequenced samples. The rapid and sensitive MAMA-PCR method established in this study provides a general strategy for detecting point mutations and improving the efficiency of mutation library screening. Key points • MAMA PCR was optimized and developed to detect point mutation. • MAMA PCR greatly improves the screening efficiency of point mutation. • Attenuation of odhA expression in P12 effectively improves proline production.

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“…A MAMA is a PCR-based technique for SNP discrimination that uses SNP-specific primers at the 3  -end. 2 We selected candidate genes according to a previous publication. 1 One forward primer was designed to be complementary to the corresponding allele of the cpsK gene of serotypes 1 and 1/2, with one mismatch introduced at the second nucleotide from the 3  -end of the primer, resulting in 2 mismatches with serotypes 2 and 14 (Fig.…”

mentioning

confidence: 99%

Development of a mismatch amplification mutation assay to correctly serotype isolates of Streptococcus suis serotypes 1, 2, 1/2, and 14

et al. 2020

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Streptococcus suis is one of the most important bacterial swine pathogens worldwide and is an emerging pathogen in humans. There are 29 serotypes, and serotyping, which is based on the antigenicity of the capsular polysaccharide (CPS) or on its coding genes, is often part of routine identification and provides further information regarding S. suis virulence and zoonotic potential. Serotypes 2 and 14 possess high zoonotic potential, and serotype 1/2 is the serotype most frequently isolated from diseased pigs in North America. PCR has replaced antibody-based techniques to perform serotyping. However, traditional PCR is not able to differentiate serotype 2 from 1/2 and serotype 1 from 14, given that the only difference in the cps loci of those serotype pairs is a nonsynonymous single-nucleotide polymorphism. We developed a mismatch amplification mutation assay (MAMA)-PCR that was able to correctly serotype 148 isolates previously known to be serotypes 1, 2, 1/2, or 14. This technique will be highly useful in animal and human health laboratories performing PCR serotyping of S. suis isolates.

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Mismatch amplification mutation assay-polymerase chain reaction

Cited by 7 publications

References 20 publications

Structural Characterization of the Millennial Antibacterial (Fluoro)Quinolones—Shaping the Fifth Generation

Structural Characterization of the Millennial Antibacterial (Fluoro)Quinolones—Shaping the Fifth Generation

Rapid screening of point mutations by mismatch amplification mutation assay PCR

Development of a mismatch amplification mutation assay to correctly serotype isolates of Streptococcus suis serotypes 1, 2, 1/2, and 14

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