A dissolved oxygen measurement using an electrochemical biosensor and conventional current mirror with an accurate result and desired sensitivity is difficult to achieve, though this current mirror is used frequently to process the biosensor signal and providing good response. However, it exhibits some drawbacks particularly due to mismatched-transistors, which will lead to asymmetry between input and output currents. This asymmetry causes the unwanted offset and gain error, making reduction of its accuracy, especially on very low current. A modified current mirror utilizing precise gate voltage adjustment of FET transistors is applied to match the transistors’ currents. The results show accuracy improvement of the modified current mirror compared to the conventional current mirror, where the improvements provide a very low accuracy error of 0.01%. In addition, the current mirror’s sensitivity can be adjusted by implementing this modification without increasing much noise.