Missense and nonsense mutations of the zebrafish hcfc1a gene result in contrasting mTor and radial glial phenotypes

Castro, Victoria L.; Paz, David; Virrueta, Valeria; Estevao, Igor L.; Grajeda, Brian; Ellis, Cameron; Quintana, Anita M.

doi:10.1016/j.gene.2023.147290

Cited by 3 publications

(1 citation statement)

References 43 publications

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“…EVs from H. capsulatum yeasts treated or not with 1 mM caffeine were subjected to proteomic analysis via nano-high-performance liquid chromatography coupled to tandem mass spectrometry (nanoHPLC-MS/MS), essentially as previously described [39][40][41][42], with some modifications. Briefly, fungal EV samples (50 µg protein) were redissolved in 200 µL 100 mM Tris-HCl pH 7.0, containing 8 M urea (urea sample solution, USS).…”

Section: Proteomic Analysis Of Fungal Evsmentioning

confidence: 99%

Traversing the Cell Wall: The Chitinolytic Activity of Histoplasma capsulatum Extracellular Vesicles Facilitates Their Release

Valdez,

de Souza,

Bonilla

et al. 2023

JoF

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Histoplasma capsulatum is the causative agent of histoplasmosis. Treating this fungal infection conventionally has significant limitations, prompting the search for alternative therapies. In this context, fungal extracellular vesicles (EVs) hold relevant potential as both therapeutic agents and targets for the treatment of fungal infections. To explore this further, we conducted a study using pharmacological inhibitors of chitinase (methylxanthines) to investigate their potential to reduce EV release and its subsequent impact on fungal virulence in an in vivo invertebrate model. Our findings revealed that a subinhibitory concentration of the methylxanthine, caffeine, effectively reduces EV release, leading to a modulation of H. capsulatum virulence. To the best of our knowledge, this is the first reported instance of a pharmacological inhibitor that reduces fungal EV release without any observed fungicidal effects.

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Section: Proteomic Analysis Of Fungal Evsmentioning

confidence: 99%