Missense Mutation inAbcg5in SHRSP Rats Does Not Accelerate Intestinal Absorption of Plant Sterols: Comparison with Wistar Rats

Missense Mutation ofAbcg5in Stroke-Prone Spontaneously Hypertensive Rats Does Not Influence Lymphatic Sitosterol Absorption Regardless of the Dose: Comparison with Wistar Rats

“…We therefore thought that the reason why no difference in absorption was apparent in sitosterol and campesterol could have been due to the relatively greater absorbed amounts in that study. 9) If the absorbed amounts were relatively low, a difference in absorption might be observed as reported by us 5) and Batta et al 8) in studies with a trace amount of 3 Hsitosterol given. In the present study, the lymphatic recovery of various amounts of sitosterol administered to the stomach is compared between SHRSPs and Wistar rats.…”

“…A second indwelling catheter was placed in the stomach for the administration of a test emulsion. 9) Three types of test emulsion were prepared. The composition of one emulsion was 67 mg of sodium taurocholate (>97%, Nacalai Tesque, Kyoto, Japan), 17 mg of bovine serum albumin, 67 mg of triolein (Sigma-Japan, Tokyo, Japan), and 122 kBq (65 ng) of y To whom correspondence should be addressed.…”

Factors Affecting Intestinal Absorption of Cholesterol and Plant Sterols and Stanols

“…Only a tendency of slightly higher absorption of sitosterol was observed in mutant rat strains. Furthermore, when Wistar rats were used as a control rat strain, there was no significant difference in lymphatic absorption of sitosterol and campesterol between SHRSP/Izm and Wistar rats 13,20 . Biliary secretion of plant sterols when fed a plant sterol diet was almost the same between SHRSP/Izm and WKY/Izm rats 19 .…”

“…We performed a similar experiment in Wistar rats, in which a bile salt micellar solution containing radiolabeled cholesterol and sitosterol was infused into the jejunal loop in situ. The incorporation of radioactivity into the jejunal loop was measured at 5, 10, 20, 60, 120, and 240 min after the infusion of the micelles 13 . The incorporation of sitosterol into the jejunal loop was only one tenth to one fifth that of cholesterol at 5, 10, and 20 min after administration of micellar sterols into the loop.…”

“…We also examined whether sitosterol is transiently incorporated into intestinal mucosa in SHRSP and compared with that in Wistar rats 13 . The results from Wistar rats were described in section 2.2.…”