Misshapen-like kinase 1 (MINK1) Is a Novel Component of Striatin-interacting Phosphatase and Kinase (STRIPAK) and Is Required for the Completion of Cytokinesis

Abstract

Background: Cytokinesis is regulated by phosphorylation and dephosphorylation of proteins. Results: MINK1 associated with STRN4, a regulatory subunit of PP2A, and depletion of either protein inhibited completion of cytokinesis. Conclusion: MINK1 and STRN4 are required for abscission, the final stage of cytokinesis. Significance: Our study reveals novel regulatory mechanisms for abscission.

Germinal center kinases in immune regulation

“…It is worth noting that the STRIPAK complex may also interact with GCK-II kinases MST1/2 and GCK-IV kinase misshapen/NIK-related kinase. 97,98 Thus, it remains an open question whether PDCD10 and GCK-III as components of the STRIPAK complex could cross-talk with GCK-II and GCK-IV signaling pathways.…”

“…The GCK-IV subfamily member misshapen/NIK-related kinase, a novel component of the STRIPAK complex, 98 is highly expressed in hematopoietic tissues and can activate the JNK signaling pathway. 120 The expression of NIK, another GCK-IV subfamily member can be selectively stimulated by TNF-a through a TNFR1-dependent mechanism.…”

Striatins Contain a Noncanonical Coiled Coil That Binds Protein Phosphatase 2A A Subunit to Form a 2:2 Heterotetrameric Core of Striatin-interacting Phosphatase and Kinase (STRIPAK) Complex

“…Several GCKs have been identified as components of the STRIPAK complex, including MST1/2, MST3, MST4, STK25, and Misshapen-like kinase 1 (MINK) (1,2,9,10,13). Diverse biological functions have been documented for these kinases.…”

STRIPAK complexes: Structure, biological function, and involvement in human diseases

“…For example, the GCKIV subfamily kinase Misshapen (Msn)-like kinase 1 (Mink1) was identified as a new STRIPAK component (Fig. 3 and Table 1) on the basis that it associates with zinedin and a number of the other established components of STRIPAK complexes (Hyodo et al, 2012). Mink1 is important for cytokinesis, particularly for the abscission process, and knockdown of either Mink1 or zinedin in HeLa cells produces multinucleated cells as a result of abnormal abscission (Hyodo et al, 2012).…”