Mississippi-grown marihuana —Cannabis sativa cultivation and observed morphological variations

Quimby, Maynard W.; Doorenbos, Norman J.; Turner, Carlton E.; Masoud, Asaad N.

doi:10.1007/bf02862224

Cited by 23 publications

(11 citation statements)

References 5 publications

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“…Commonly the leafy parts of the inflorescence are analysed to obtain an overall cannabinoid profile (Hemphill et al, 1980;Quimby et al, 1973;Avico & Zuccaro, 1984). In our experiment the upper 30 cm of the main stem inflorescence was collected for analysis.…”

Section: Sampling and Sample Treatment For Chemical Characterisationmentioning

confidence: 99%

Characterisation of Cannabis accessions with regard to cannabinoid content in relation to other plant characters

Kamp²,

1992

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Ninety seven Cannabis accessions were evaluated for cannabinoid content and non-chemical plant characters. Variation within populations for cannabinoid content, and consistency of chemical characters at the population level were investigated. The relationship between chemical and other plant characters was very limited. Leaflet width and phenological data can be used for a rough prediction of the chemical phenotype on a population level. Various combinations of cannabinoid content and other economic plant characters were observed, thus a breeding programme will not be hampered by strict linkage. For a selection programme a direct analysis of cannabinoids will be inevitable.

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Section: Sampling and Sample Treatment For Chemical Characterisationmentioning

confidence: 99%

Characterisation of Cannabis accessions with regard to cannabinoid content in relation to other plant characters

Kamp²,

1992

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“…The morphological, geographical, and chemical features of C. sativa are highly variable. Several studies have examined the variations in morphology and chemical content of C. sativa and, in particular, variations in the cannabinoid content [1][2][3][4]. Genetic variations in cannabis populations have been widely distributed throughout the world via commerce and illicit Internet trade.…”

Section: Introductionmentioning

confidence: 99%

DNA polymorphisms in the tetrahydrocannabinolic acid (THCA) synthase gene in “drug-type” and “fiber-type” Cannabis sativa L.

Kojoma

Seki

Yoshida

et al. 2006

Forensic Science International

146

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“…Since no considerable variations in the morphology of the afore-mentioned categories of the plant have been documented, a number of molecular tools have been utilized for the characterization of cannabis (Quimby et al, 1973;Small and Cronquist, 1976;Rowan and Fairbairn, 1977;Baker et al, 1982) including Inter-Simple Sequence Repeat amplification (ISSR) (Kojoma et al, 2002), Short Tandem Repeat microsatellite (STR) (Hsieh et al, 2003;Alghanim and Almirall, 2003), Amplified Fragment Length Polymorphism (AFLF) (Miller et al, 2003), 5S-rRNA gene spacer region (Miyahara et al, 1998) and Random Amplified Polymorphism (RAPD) (Gillan et al, 1995;Jagadish et al, 1996;Gigliano et al, 1995;de Meijer et al, 2003). The machinery involved in the biosynthesis of Δ-9tetrahydrocannabinolic acid (THCA) was unraveled in 1995 by Taura et al In addition, the discovery of THCA synthase gene has paved the way for detailed analysis of the narcotic material in cannabis (Sirikantaramas et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

The identification of SNPs in THCA synthase gene from Pakistani Cannabis

Ali

Mufti

et al. 2019

APJMBB

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Five different isolates of Pakistani cannabis belonging to varying locations were analyzed for the presence of a reported tetrahydrocannabinolic acid (THCA) synthase marker or gene. The amplification of the marker (12 kb) from the five isolates confirmed them to be drug-type since the association of the marker with drug-type cannabis plants had already been established in other reports. The sequence analysis of the THCA synthase marker revealed two single nucleotide polymorphisms (SNPs) (i.e. A851→T851 and A883→C883) specific to Pakistani drug-type cannabis. Furthermore, the predicted protein sequence of the isolated sequences also showed two amino acid substitutions (D284→V284 and T295→P295) corresponding to the identified SNPs. However, the homology based three dimensional models of the inferred proteins generated via Swiss-Model-an automated online server did not project any changes at the active sites of the enzyme (THCA) due to D→V and T→P substitutions. The two missense mutations uncovered as a result of this study may assist in distinguishing the products of Pakistani cannabis among the smuggled materials.

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Mississippi-grown marihuana —Cannabis sativa cultivation and observed morphological variations

Cited by 23 publications

References 5 publications

Characterisation of Cannabis accessions with regard to cannabinoid content in relation to other plant characters

Characterisation of Cannabis accessions with regard to cannabinoid content in relation to other plant characters

DNA polymorphisms in the tetrahydrocannabinolic acid (THCA) synthase gene in “drug-type” and “fiber-type” Cannabis sativa L.

The identification of SNPs in THCA synthase gene from Pakistani Cannabis

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