Mist culture for mass harvesting of root border cells: aluminum effects

Yu, Min; Ying, Feng; Goldbach, Heiner E.

doi:10.1002/jpln.200620604

Cited by 19 publications

(30 citation statements)

References 27 publications

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“…5) were germinated under mist culture (Yu et al 2006). Pea seeds were immersed in 95% ethanol for 10 min, followed by 5.25% sodium hypochlorite for 30 min, and rinsed six times with de-ionized water according to Brigham et al (1995).…”

Section: Seed Germinationmentioning

confidence: 99%

Boron alleviates aluminum toxicity in pea (Pisum sativum)

Shen

Xiao

et al. 2008

Plant Soil

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One important target of boron (B) deficiency and aluminum (Al) toxicity is cell wall. Thus we studied the hypothesis that B is capable of alleviating Al toxicity in pea (Pisum sativum). Short-term and prolonged Al exposure to pea roots at different B levels was carried out on uniform seedlings precultured at a low B level. When seedlings with a low B level were supplied with or without B for 1 and 2 days before 24 h Al exposure, roots were longer while root diameter was thinner after B addition especially for 2 days even with exposure to Al; root elongation was inhibited while root diameter was enlarged by Al exposure. Callose induction by Al toxicity was higher with B added, but this was reversed after the removal of the cotyledons. Hematoxylin staining was lighter in the root tips given B, and Al content in the root tips and cell walls dropped after exposure to B. This indicates that B alleviated Al toxicity in the root tips during short-term Al exposure by decreasing Al binding in root cell walls. An increase in chlorophyll and biomass and reduced chlorosis were found at the higher level of B during prolonged Al treatment, which was coincided with the decreased Al contents, indicating that B alleviated Al toxicity to shoots. B supplementation alleviates some of the consequences of Al toxicity by limiting some Al binding in cell walls, resulting in less injury to the roots as well as less injury to the shoots.

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Section: Seed Germinationmentioning

confidence: 99%

Boron alleviates aluminum toxicity in pea (Pisum sativum)

Shen

Xiao

et al. 2008

Plant Soil

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“…This number is conserved in each plant family and is strictly regulated by compounds released from the border cells . When germinating seeds were kept in mist culture, the number of border cells was found to be approximately 10,000 per root in Pisum sativum (Yu et al, 2006), which is markedly higher than previously reported . Root border cells are thought to have crucial physiological and ecological functions in protecting root apices from chemical (e.g., aluminum [Al]), physical, and biological stress (Fiskesjo, 1990;Hawes et al, 1998).…”

Section: Introductionmentioning

(Expert classified)

“…Zhongwan No. 5) were germinated under mist culture, and border cells were harvested as described previously (Yu et al, 2006). Pea seeds were immersed in 95% ethanol for 10 min, followed by 5.25% sodium hypochlorite for 30 min, and finally rinsed six times with deionized water as described by Brigham et al (1995).…”

Section: Plant Materialsmentioning

confidence: 99%

“…It was thus hypothesized that root border cells could enhance the Al resistance of root apices as an Al-immobilizing barrier (Fiskesjo, 1990;Hawes et al, 1998;Tamas et al, 2005). Mist culture (Yu et al, 2006) is a culture method that keeps border-cell layers intact around root apices, allowing modifying the supply of nutrients as well as of toxic compounds to the roots. Here, we used this method to investigate the role of border cells in the protection of root apices against Al toxicity.…”

Section: Introductionmentioning

confidence: 99%

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The role of root border cells in aluminum resistance of pea (Pisum sativum) grown in mist culture

Shen

Liu

et al. 2009

Z. Pflanzenernähr. Bodenk.

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Root border cells are considered to contribute to aluminum (Al) resistance by protecting the root apex from Al toxicity. In the present study, the responses of root apices of pea (Pisum sativum) to Al exposure in mist culture with border cells stripped off or not were compared. Inhibition of root elongation, induction of callose synthesis, and accumulation of Al were more pronounced in root apices stripped from border cells. Aluminum application led to higher Al concentrations in border cells than in root apices. The same trend was found for Al contents in cell walls of border cells compared to root apices. The analysis of cell‐wall pectin indicated that the concentrations of total sugars, uronic acids, and 2‐keto‐3‐deoxyoctonic acid (KDO) were higher in border cells than in root apices, especially when exposed to Al. Together, these results suggest that root border cells enhance the Al resistance of root apices by immobilizing Al in their cell‐wall pectin, thus protecting the root apex.

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“…The removal of RBCs from the root tips of wheat [22] or pea [17] enhanced the Al-induced inhibition of root elongation concomitant with increased Al accumulation in the root. This function is possibly performed through exudation of an Al-binding mucilage from the RBCs [16,17,23]. In addition, maize mucilage separated from the root tips have been shown to tightly bind Al.…”

Section: Introductionmentioning

confidence: 99%