Mitigating ammonia-inhibition in anaerobic digestion by bioaugmentation: A review

Li, Ziyan; Inoue, Daisuke; Ike, Michihiko

doi:10.1016/j.jwpe.2023.103506

Cited by 38 publications

(12 citation statements)

References 95 publications

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“…Metagenomic analyses were carried out as previously described . Briefly, the reads were coassembled using MEGAHIT v1.2.9 .…”

Section: Methodsmentioning

confidence: 99%

“…Metagenomic analyses were carried out as previously described. 12 Briefly, the reads were coassembled using MEGAHIT v1.2.9. 16 The depth file for the binning analyses was generated with Bowtie2 v2.4.5.…”

Section: Experimental Setup and Processmentioning

confidence: 99%

“…11 The VFA produced during the acidogenesis step cannot flow into the methanogenesis process, resulting in their accumulation. 12 The present study aimed to unveil the early response of the AD microbiome to the introduction of ammonia shock loads in biogas reactors, employing a combined biochemical, genomecentric metagenomic, and metatranscriptomic approach. The investigation was conducted using triplicate continuous stirred tank reactors (CSTRs) exposed to ammonia shock loads induced by single pulses at two different concentrations.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, a side effect of increased ammonia levels is the accumulation of VFA and H 2 . The VFA produced during the acidogenesis step cannot flow into the methanogenesis process, resulting in their accumulation …”

Section: Introductionmentioning

confidence: 99%

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Decoding Microbial Responses to Ammonia Shock Loads in Biogas Reactors through Metagenomics and Metatranscriptomics

Gaspari,

Ghiotto,

Centurion

et al. 2023

Environ. Sci. Technol.

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The presence of elevated ammonia levels is widely recognized as a significant contributor to process inhibition in biogas production, posing a common challenge for biogas plant operators. The present study employed a combination of biochemical, genome-centric metagenomic and metatranscriptomic data to investigate the response of the biogas microbiome to two shock loads induced by single pulses of elevated ammonia concentrations (i.e., 1.5 g NH 4 + /L R and 5 g NH 4 + /L R ). The analysis revealed a microbial community of high complexity consisting of 364 Metagenome Assembled Genomes (MAGs). The hydrogenotrophic pathway was the primary route for methane production during the entire experiment, confirming its efficiency even at high ammonia concentrations. Additionally, metatranscriptomic analysis uncovered a metabolic shift in the methanogens Methanothrix sp. MA6 and Methanosarcina f lavescens MX5, which switched their metabolism from the acetoclastic to the CO 2 reduction route during the second shock. Furthermore, multiple genes associated with mechanisms for maintaining osmotic balance in the cell were upregulated, emphasizing the critical role of osmoprotection in the rapid response to the presence of ammonia. Finally, this study offers insights into the transcriptional response of an anaerobic digestion community, specifically focusing on the mechanisms involved in recovering from ammonia-induced stress.

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“…Metagenomic analyses were carried out as previously described . Briefly, the reads were coassembled using MEGAHIT v1.2.9 .…”

Section: Methodsmentioning

confidence: 99%

Section: Experimental Setup and Processmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Decoding Microbial Responses to Ammonia Shock Loads in Biogas Reactors through Metagenomics and Metatranscriptomics

Gaspari,

Ghiotto,

Centurion

et al. 2023

Environ. Sci. Technol.

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“…All these families are well-known inhabitants of mesophilic digesters [57][58][59], covering three of the four main steps of AD. While Prolixibacteraceae is mainly involved in the initial hydrolysis [60], Bacteroidetes vadinHA17 has been described to decompose organic material further down to acetic acid, propionate and hydrogen/carbon dioxide [61]. Peptostreptococcaceae belongs to the group of syntrophic acetate-oxidizing bacteria (SAOB) [62] and is tightly connected to hydrogenotrophic methanogens.…”

Section: Bacterial Taxonomymentioning

confidence: 99%

Exploring Anaerobic Digestion from Mesophilic to Thermophilic Temperatures—Operational and Microbial Aspects

Steiniger,

Hupfauf,

Insam

et al. 2023

Fermentation

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Digesters at water resource recovery facilities (WRRFs) operating at different temperatures within the mesophilic and thermophilic temperature range is a flexibilization concept to contribute to heat management. Four 25 L digesters were fed with sewage sludge from a municipal WRRF and were operated at 37, 43, 47 and 53 °C, respectively, to describe changes in the overall process performance and the microbiota. Specific methane yield and COD degradation rates were the highest at 47 °C, only being up to 7% higher compared with at 37 °C. The increase in pH and concentrations of NH4-N and PO4-P above 43 °C were statistically significant. The effect on the microbial community was strong, indicating both a constant specialization towards thermophilic organisms as well as a change from acetoclastic to hydrogenotrophic/methylotrophic methanogenesis. The influence of temperature on process-engineering and physicochemical aspects was rather small compared with the changes in the microbiota.

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Enhancement of anaerobic digestion of dairy wastewater by addition of conductive materials with or without the combination of external voltage application

Fountoulakis,

Frkova,

Lemaigre

et al. 2024

J of Chemical Tech & Biotech

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BACKGROUNDThe addition of conductive materials or the application of external voltage is a promising novel methodology to enhance methane production in anaerobic digesters. However, there is limited knowledge about their individual or combined effects on the anaerobic digestion of dairy wastewater. The aim of this study was to examine the effects of granular activated carbon (GAC) or magnetite addition and external voltage application, both individually and in combination, for the anaerobic digestion of real, undiluted dairy wastewater.RESULTSThe results showed that the estimated maximum methane production rate increased significantly (P < 0.05) from 143 ± 8 mL gVSadded−1 day−1 in the control reactors to 163 ± 11 mL gVSadded−1 day−1 and 166 ± 11 mL gVSadded−1 day−1 in the reactors containing GAC, with or without the combination of external voltage, respectively. The addition of GAC, in both cases, resulted in higher consumption rate of acetate, indicating a promotion of the methanogenesis step. By contrast, the application of external voltage or the addition of magnetite had no significant effect on either methane production rate nor methane yield. Moreover, the analysis of the microbial community showed that the addition of GAC resulted in the enrichment of Desulfobacterota, Methanosarcina, Candidatus Methanofastidiosum and Methanolinea.CONCLUSIONOverall, GAC addition seems a promising strategy to increase methane production in anaerobic digesters treating dairy wastewater. © 2024 The Author(s). Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

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Mitigating ammonia-inhibition in anaerobic digestion by bioaugmentation: A review

Cited by 38 publications

References 95 publications

Decoding Microbial Responses to Ammonia Shock Loads in Biogas Reactors through Metagenomics and Metatranscriptomics

Decoding Microbial Responses to Ammonia Shock Loads in Biogas Reactors through Metagenomics and Metatranscriptomics

Exploring Anaerobic Digestion from Mesophilic to Thermophilic Temperatures—Operational and Microbial Aspects

Enhancement of anaerobic digestion of dairy wastewater by addition of conductive materials with or without the combination of external voltage application

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