Mitigating the Antimicrobial Activities of Selected Organic Acids and Commercial Sanitizers with Various Neutralizing Agents

Park, Yoen Ju; Chen, Jinru

doi:10.4315/0362-028x.jfp-10-447

Cited by 13 publications

(8 citation statements)

References 22 publications

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“…All disinfectants were freshly prepared according to the manufacturer's instructions prior to testing. The corresponding neutralizing agents for each disinfectant were also freshly prepared [ 12 ]. Sterile distilled water was used as a diluent and as a disinfectant control.…”

Section: Methodsmentioning

confidence: 99%

In vitro evaluation of six chemical agents on smooth Brucella melitensis strain

Wang

Bie

Cheng

et al. 2015

Ann Clin Microbiol Antimicrob

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Brucellosis is a zoonosis that disseminated by a variety of ways between animals and humans. The effective disinfection of contaminated environments, soil, feces, and animal bodies plays an irreplaceable role in the prevention and control of brucellosis. To kill Brucella effectively, the bactericidal effects of frequently used disinfectants (including aldehydes, halogens, quaternary ammonium compound, phenolics, and alkalines) and the potential factors that influence disinfection effects were determined in the present study. The results revealed that the minimum bactericidal concentrations (MBCs) of the six disinfectants were all significantly lower than the routinely used concentrations, and all the tested disinfectants were effective against B. melitensis NI. The results of quantitative determination showed that the bactericidal effects of the disinfectants were influenced by their concentration, exposure time, dirty condition and the temperature. Under dirty conditions and a low temperatures, sodium hypochlorite and sodium hydroxide showed better bactericidal effect, while benzalkonium chloride was almost without bactericidal ability. In addition, increasing the disinfectant concentration at low temperatures can improve the bactericidal effect. The present study suggested that Brucella is sensitive to commonly used disinfectants. However, the bactericidal effect is vulnerable to dirty conditions and low temperatures. Thus, it is necessary to test the in vitro sensitivity of disinfectants that are commonly used on farms or the new disinfectant formulations periodically, with the aim of improving the efficacy of animal and human brucellosis prevention programs.

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Section: Methodsmentioning

confidence: 99%

In vitro evaluation of six chemical agents on smooth Brucella melitensis strain

Wang

Bie

Cheng

et al. 2015

Ann Clin Microbiol Antimicrob

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“…Only the latter five treatments were used in the hide decontamination model. Morpholinepropanesulfonic acid (MOPS) was used for neutralization of low pH after chemical treatments (Park and Chen, 2011).…”

Section: Reagent Preparationmentioning

confidence: 99%

Synergistic Effects of Lactic Acid and Sodium Dodecyl Sulfate to Decontaminate Escherichia coli O157:H7 on Cattle Hide Sections

Elramady

Aly

Rossitto

et al. 2013

Foodborne Pathogens and Disease

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The objective of this study was to investigate the antibacterial properties of chitosan acetate (CA), sodium dodecyl sulfate (SDS), lactic acid (LA) and their synergism when combined against a nontoxigenic strain of Escherichia coli O157:H7. Treatments that significantly reduced the concentration of E. coli O157:H7 in vitro by more than two logs were further investigated using a cattle hide decontamination model. In vitro treatments included CA (1% chitosan in 1% acetic acid vol/vol), SDS (1% vol/vol), SDS (2% vol/vol), LA (1% vol/vol), CA-SDS combination (1% chitosan in 1% acetic acid vol/vol mixed with 1% SDS vol/vol), and LA-SDS combination in two different concentrations (1% LA mixed with 1% SDS vol/vol, and 1% LA mixed with 2% SDS vol/vol). Butterfield's Phosphate Buffer water was used as a control. The antibacterial effect of 1% CA solution alone and in combination with 1% SDS in vitro resulted in a 1.8 and 1.7 log colony-forming units (CFU)/mL reduction, respectively ( p < 0.05). Only 1% LA, 1% SDS, 2% SDS and their combinations resulted in a > 2 log reduction in E. coli O157:H7. On hide sections, both 1% LA-1% SDS and 1% LA-2% SDS combinations significantly ( p < 0.05) reduced E. coli O157:H7 concentration by 4.6 and 4.7 log CFU/ cm 2 greater than the control, respectively. There was no significant difference in the antibacterial effect of 1% LA compared to the control, 2% SDS compared to the control, or 1% LA compared to 2% SDS. Hence, the antibacterial efficacy of 1% LA against E. coli O157:H7 on hide sections was significantly enhanced when combined with 1% SDS. Results of this study support the use of low concentration LA-SDS combination as a hide wash to reduce the risk of E. coli O157:H7 contamination.

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“…Transport broths also typically contain agents that can neutralize pH. Previous studies have compared the neutralizing capacity of various buffer as potential transport media, even though their experimental designs were different from the current study [ 14 , 19 ]. Park et al evaluated several transport broths against three acidic sanitizers and one alkaline sanitizer by mixing transport broths with sanitizers before adding liquid Shiga toxin-producing Escherichia coli (STEC) cultures.…”

Section: Resultsmentioning

confidence: 97%

“…Such sanitizer residues could cause false negative results in environmental testing. A variety of neutralizing agents were used in transport broths, such as lecithin, polysorbate, sodium thiosulfate, sodium bisulfite, and aryl sulphonate complex [ 14 , 19 – 21 ]. Transport broths also typically contain agents that can neutralize pH.…”

Section: Resultsmentioning

confidence: 99%

Comparison of three neutralizing broths for environmental sampling of low levels of Listeria monocytogenes desiccated on stainless steel surfaces and exposed to quaternary ammonium compounds

Xian

Kwon

et al. 2020

BMC Microbiol

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Background An effective environmental sampling method involves the use of a transport/neutralizing broth with the ability to neutralize sanitizer residues that are collected during sampling and to maintain viability of stressed Listeria monocytogenes (Lm) cells. Results We applied Lm onto stainless steel surfaces and then subjected Lm to desiccation stress for 16–18 h at room temperature (RT, 21–24 °C). This was followed by the subsequent application of Whisper™ V, a quaternary ammonium compound (QAC)-based sanitizer, diluted to 400 ppm and 8000 ppm of active quat, for 6 h. We then sampled Lm with sponges pre-moistened in three transport broths, Dey/Engley (D/E) broth, Letheen broth and HiCap™ broth, to generate environmental samples that contained sanitizer residues and low levels of stressed Lm, which were subsequently analyzed by an enrichment-based method. This scheme conformed with validation guidelines of AOAC International by using 20 environmental test portions per broth that contained low levels of Lm such that not all test portions were positive (i.e., fractional positive). We showed that D/E broth, Letheen broth and HiCap™ broth performed similarly when no quat or 400 ppm of quat was applied to the Lm contaminating stainless steel surfaces. However, when 8000 ppm of quat was applied, Letheen broth did not effectively neutralize the QAC in the samples. These comparisons were performed on samples stored under three conditions after collection to replicate scenarios of sample transport, RT for 2 h, 4 °C for 24 h and 4 °C for 72 h. Comparisons under the three different scenarios generally reached the same conclusions. In addition, we further demonstrated that storing Letheen and HiCap™ broths at RT for two months before sampling did not reduce their capacity to neutralize sanitizers. Conclusions We developed a scheme to evaluate the ability of transport broths to neutralize QAC sanitizers. The three transport broths performed similarly with a commonly used concentration of quat, but Letheen broth could not effectively neutralize a very high concentration of QAC. The performance of transport broths was not significantly affected under the assessed pre-sampling and post-sampling storage conditions.

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Mitigating the Antimicrobial Activities of Selected Organic Acids and Commercial Sanitizers with Various Neutralizing Agents

Cited by 13 publications

References 22 publications

In vitro evaluation of six chemical agents on smooth Brucella melitensis strain

In vitro evaluation of six chemical agents on smooth Brucella melitensis strain

Synergistic Effects of Lactic Acid and Sodium Dodecyl Sulfate to Decontaminate Escherichia coli O157:H7 on Cattle Hide Sections

Comparison of three neutralizing broths for environmental sampling of low levels of Listeria monocytogenes desiccated on stainless steel surfaces and exposed to quaternary ammonium compounds

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