Mitigation of Laughing Gas Emissions by Nitrous Oxide Respiring Microorganisms

Simon, Jörg

doi:10.1007/978-3-030-58315-6_7

Cited by 3 publications

(6 citation statements)

References 114 publications

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“…N 2 O reducing bacteria are often facultatively aerobic bacteria isolated from anoxic to microoxic habitats that possess a respiratory chain involved in micro-aerobic respiration. It is assumed that this property gives the cells a certain aerotolerance and protects the N 2 O reductase from inactivation by O 2 [6]. This could be an explanation for the moderate consumption of N 2 O under microoxic conditions with decreasing O 2 content in this experiment.…”

Section: Consumption Of N 2 Omentioning

confidence: 71%

“…N2O reduction by NosZ usually requires anoxic conditions, as NosZ copper centers are susceptible to O2 [6]. Nevertheless, N2O reduction under oxic and microoxic conditions has been described, for example, for strains of the species Pseudomonas stutzeri (Clade I The nosZ gene of strain IGB 4-14 T is followed by genes encoding NosZ asseccory proteins annotated as nosL (M0M57_RS11110), nosD (M0M57_RS11115), nosF (M0M57_RS11120) and nosY (M0M57_RS11125).…”

Section: Consumption Of N2omentioning

confidence: 99%

Section: Consumption Of N 2 Omentioning

confidence: 99%

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Consumption of N2O by Flavobacterium azooxidireducens sp. nov. Isolated from Decomposing Leaf Litter of Phragmites australis (Cav.)

et al. 2022

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Microorganisms acting as sinks for the greenhouse gas nitrous oxide (N2O) are gaining increasing attention in the development of strategies to control N2O emissions. Non-denitrifying N2O reducers are of particular interest because they can provide a real sink without contributing to N2O release. The bacterial strain under investigation (IGB 4-14T), isolated in a mesocosm experiment to study the litter decomposition of Phragmites australis (Cav.), is such an organism. It carries only a nos gene cluster with the sec-dependent Clade II nosZ and is able to consume significant amounts of N2O under anoxic conditions. However, consumption activity is considerably affected by the O2 level. The reduction of N2O was not associated with cell growth, suggesting that no energy is conserved by anaerobic respiration. Therefore, the N2O consumption of strain IGB 4-14T rather serves as an electron sink for metabolism to sustain viability during transient anoxia and/or to detoxify high N2O concentrations. Phylogenetic analysis of 16S rRNA gene similarity revealed that the strain belongs to the genus Flavobacterium. It shares a high similarity in the nos gene cluster composition and the amino acid similarity of the nosZ gene with various type strains of the genus. However, phylogenomic analysis and comparison of overall genome relatedness indices clearly demonstrated a novel species status of strain IGB 4-14T, with Flavobacterium lacus being the most closely related species. Various phenotypic differences supported a demarcation from this species. Based on these results, we proposed a novel species Flavobacterium azooxidireducens sp. nov. (type strain IGB 4-14T = LMG 29709T = DSM 103580T).

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Section: Consumption Of N 2 Omentioning

confidence: 71%

Section: Consumption Of N2omentioning

confidence: 99%

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Consumption of N2O by Flavobacterium azooxidireducens sp. nov. Isolated from Decomposing Leaf Litter of Phragmites australis (Cav.)

et al. 2022

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“…Increasing the soils’ population of N 2 O-respiring bacteria ( NRB , BOX 1) could decrease the emission of N 2 O (Simon 2021). NRB with a complete denitrification pathway can be net sinks of N 2 O if their denitrification regulatory networks secure earlier and/or stronger expression of NosZ than of the other denitrification enzymes (Lycus et al 2018, Jonassen et al 2022a), or if their electron flow is channeled preferentially to NosZ (Gao et al 2021).…”

Section: The Nitrogen Footprints Of Farmingmentioning

confidence: 99%

“…The capacity of a strain to reduce N 2 O-emissions is commonly judged by a set of biokinetic parameters (Simon 2021), and we decided to investigate these for CB-01, for comparison with other strains.…”

Section: The Nitrogen Footprints Of Farmingmentioning

confidence: 99%

Effective biotechnology for reducing N₂O-emissions from farmland: N₂O-respiring bacteria vectored by organic waste

Hiis,

Vick,

Molstad

et al. 2023

Preprint

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Farmed soils contribute to global warming primarily by N2O-emissions, and mitigation has proven difficult. However, a novel approach with promising results in the laboratory, exploits organic wastes both as substrates and vectors for strains of N2O-respiring bacteria (NRB), selected for their ability to survive in soil. Here we demonstrate a strong effect in field experiments: fertilization with waste from biogas-production, in which the strain Cloacibacterium sp. CB-01 had grown aerobically to ~6*109 cells mL-1, reduced N2O-emissions by 50-95 %. The strong and long-lasting effect of CB-01 is ascribed to its tenacity in soil, rather than its biokinetic parameters, which were inferior to other NRB-strains. Scaling up to EU level, we find that national anthropogenic N2O-emissions can be reduced by 5-20 %, and more if including other organic wastes. This opens an avenue for cost-effective reduction of N2O-emissions for which other mitigation options are currently lacking.

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Unlocking bacterial potential to reduce farmland N2O emissions

Hiis,

Vick,

Molstad

et al. 2024

Nature

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Farmed soils contribute substantially to global warming by emitting N2O (ref. 1), and mitigation has proved difficult2. Several microbial nitrogen transformations produce N2O, but the only biological sink for N2O is the enzyme NosZ, catalysing the reduction of N2O to N2 (ref. 3). Although strengthening the NosZ activity in soils would reduce N2O emissions, such bioengineering of the soil microbiota is considered challenging4,5. However, we have developed a technology to achieve this, using organic waste as a substrate and vector for N2O-respiring bacteria selected for their capacity to thrive in soil6–8. Here we have analysed the biokinetics of N2O reduction by our most promising N2O-respiring bacterium, Cloacibacterium sp. CB-01, its survival in soil and its effect on N2O emissions in field experiments. Fertilization with waste from biogas production, in which CB-01 had grown aerobically to about 6 × 109 cells per millilitre, reduced N2O emissions by 50–95%, depending on soil type. The strong and long-lasting effect of CB-01 is ascribed to its tenacity in soil, rather than its biokinetic parameters, which were inferior to those of other strains of N2O-respiring bacteria. Scaling our data up to the European level, we find that national anthropogenic N2O emissions could be reduced by 5–20%, and more if including other organic wastes. This opens an avenue for cost-effective reduction of N2O emissions for which other mitigation options are lacking at present.

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Mitigation of Laughing Gas Emissions by Nitrous Oxide Respiring Microorganisms

Cited by 3 publications

References 114 publications

Consumption of N2O by Flavobacterium azooxidireducens sp. nov. Isolated from Decomposing Leaf Litter of Phragmites australis (Cav.)

Consumption of N2O by Flavobacterium azooxidireducens sp. nov. Isolated from Decomposing Leaf Litter of Phragmites australis (Cav.)

Effective biotechnology for reducing N₂O-emissions from farmland: N₂O-respiring bacteria vectored by organic waste

Unlocking bacterial potential to reduce farmland N2O emissions

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Mitigation of Laughing Gas Emissions by Nitrous Oxide Respiring Microorganisms

Cited by 3 publications

References 114 publications

Consumption of N2O by Flavobacterium azooxidireducens sp. nov. Isolated from Decomposing Leaf Litter of Phragmites australis (Cav.)

Consumption of N2O by Flavobacterium azooxidireducens sp. nov. Isolated from Decomposing Leaf Litter of Phragmites australis (Cav.)

Effective biotechnology for reducing N2O-emissions from farmland: N2O-respiring bacteria vectored by organic waste

Unlocking bacterial potential to reduce farmland N2O emissions

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Product

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Effective biotechnology for reducing N₂O-emissions from farmland: N₂O-respiring bacteria vectored by organic waste