The ant colony algorithm is an algorithm adopted from the behavior of ants which naturally ants are able to find the shortest route on the way from the nest to places of food sources based on footprints on the track that has been passed. The ant colony algorithm helps a lot in solving several problems such as scheduling, traveling salesman problems (TSP) and vehicle routing problems (VRP). In addition, ant colony has been developed and has several variants. However, in its function to find the shortest distance is optimized by utilizing several normalized distance formulas with the data used in finding distances between merchants in the mercant ecosystem. Where in the test normalized distance is superior Hamming distance in finding the shortest distance of 0.2875, then followed by the same value, namely the normalized formula Manhattan distance and normalized Euclidean distance with a value of 0.4675 and without using the normalized distance formula or the original ant colony algorithm gets a value 0.6635. Given the sensitivity in distance search using merchant ecosystem data, the method works well on the ant colony Algorithm using normalized Hamming distance.