Mitigation of PEG-induced drought stress in rapeseed (Brassica rapa L.) by exogenous application of osmolytes

Bhuiyan, Tasnim Farha; Ahamed, Kamal Uddin; Mahmud, Jubayer Al; Bhuyan, M. H. M. Borhannuddin; Anee, Taufika Islam; Fujita, Masayuki; Hasanuzzaman, Mirza

doi:10.1016/j.bcab.2019.101197

Cited by 50 publications

(36 citation statements)

References 40 publications

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“…Tre content was determined spectrophotometrically following the method of Bhuiyan et al [62]. Data of absorbance were noted at 630 nm, and Tre concentration was calculated as micromoles per gram fresh weight (µmol g −1 FW) comparing with a standard curve of commercial Tre (Sigma-Aldrich, USA).…”

Section: Estimation Of Tre Contentmentioning

confidence: 99%

Trehalose Protects Maize Plants from Salt Stress and Phosphorus Deficiency

Rohman

Islam

Monsur

et al. 2019

Plants

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This study is undertaken to elucidate the role of trehalose (Tre) in mitigating oxidative stress under salinity and low P in maize. Eight-day-old maize seedlings of two maize varieties, BARI Hybrid Maize-7 and BARI Hybrid Maize-9, were subjected to salinity (150 mM NaCl), low P (5 µM KH2PO4) and their combined stress with or without 10 mM Tre for 15 d. Salinity and combined stress significantly inhibited the shoot length, root length, and root volume, whereas low P increased the root length and volume in both genotypes. Exogenous Tre in the stress treatments increased all of the growth parameters as well as decreased the salinity, low P, and combined stress-mediated Na+/K+, reactive oxygen species (ROS), malondialdehyde (MDA), lipoxygenase (LOX) activity, and methylglyoxal (MG) in both genotypes. Individually, salinity and low P increased superoxide dismutase (SOD) activity in both genotypes, but combined stress decreased the activity. Peroxidase (POD) activity increased in all stress treatments. Interestingly, Tre application enhanced the SOD activity in all the stress treatments but inhibited the POD activity. Both catalase (CAT) and glutathione peroxidase (GPX) activity were increased by saline and low P stress while the activities inhibited in combined stress. Similar results were found for ascorbate peroxidase (APX), glutathione peroxidase (GR), and dehydroascorbate reductase (DHAR) activities in both genotypes. However, monodehydroascorbate reductase (MDHAR) activity was inhibited in all the stresses. Interestingly, Tre enhanced CAT, APX, GPX, GR, MDHAR, and DHAR activities suggesting the amelioration of ROS scavenging in maize under all the stresses. Conversely, increased glyoxalase activities in saline and low P stress in BHM-9 suggested better MG detoxification system because of the down-regulation of glyoxalase-I (Gly-I) activity in BHM-7 in those stresses. Tre also increased the glyoxalase activities in both genotypes under all the stresses. Tre improved the growth in maize seedlings by decreasing Na+/K+, ROS, MDA, and MG through regulating antioxidant and glyoxalase systems.

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Section: Estimation Of Tre Contentmentioning

confidence: 99%

Trehalose Protects Maize Plants from Salt Stress and Phosphorus Deficiency

Rohman

Islam

Monsur

et al. 2019

Plants

Self Cite

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show abstract

“…Tre content was determined spectrophotometrically following the method of Bhuiyan et al [62]. Data of absorbance were noted at 630 nm, and Tre concentration was calculated as micromoles per gram fresh weight (µ mol g −1 FW) comparing with a standard curve of commercial Tre (Sigma-Aldrich, 90210).…”

Section: Estimation Of Tre Contentmentioning

confidence: 99%

“…POD (EC 1.11.1.7): POD activity was assayed following Hemeda and Klein [69] by guaiacol oxidation for 1 min and the activity was calculated with extinction coefficient of 26.6 mM −1 cm −1 . CAT (EC: 1.11.1.6): CAT activity was assayed with the method described in Bhuiyan et al [62]. the activity was calculated with an extinction coefficient of 39.4 M −1 cm −1 H2O2 decompositions.…”

Section: Assay Of Enzymatic Activitiesmentioning

confidence: 99%

“…The activity was calculated considering an extinction coefficient of 2.8 mM −1 cm −1 . The activities of MDHAR (EC: 1.6.5.4) and GR (EC: 1.6.4.2) were assayed following the methods of Bhuiyan et al [62] and were calculated using extinction coefficients of 6.2 mM −1 cm −1 . On the other hand, DHAR (EC: 1.8.5.1) activity was determined with the procedure of Nakano and Asada [70] where extinction coefficient of 14 mM −1 cm −1 was used to calculate the activity.…”

Section: Assay Of Enzymatic Activitiesmentioning

confidence: 99%

“…The activity was calculated using an extinction coefficient of 9.6 mM −1 cm −1 . The activities of Gly-I (EC: 4.4.1.5) and Gly-II (EC 3.1.2.6) were assayed following the methods of Yadav et al [6] and Bhuiyan et al [62], respectively. The activities were calculated using extinction coefficient of 3.37 mM −1 cm −1 and 13.6 mM −1 cm −1 , respectively.…”

Section: Assay Of Enzymatic Activitiesmentioning

confidence: 99%

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Trehalose Protects Maize Plants from Salt Stress and Phosphorus Deficiency

Rohman¹,

Islam²,

Monsur³

et al. 2019

Preprint

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This study was undertaken to elucidate the role of trehalose (Tre) in mitigating oxidative stress under salinity and low P in maize. Eight-day-old maize seedlings of two maize varieties, BARI Hybrid Maize-7 and BARI Hybrid Maize-9 were subjected to salinity (150 mM NaCl), low P (5 µM KH2PO4) and their combined stress with or without 10 mM Tre for 15-d.Salinity and combined stress significantly inhibited the shoot length, root length, and root volume, whereas, low P increased the root length and volume in both genotypes. Exogenous Tre in the stress treatments increased all of the growth parameters as well as decreased the salinity, low P and combined stress-mediated Na+/K+, ROS, MDA, LOX activity and MG in both genotypes. Under salinity and low P stress, the SOD activity increased in both genotypes, but the activity decreased in combined stress. POD activity increased in all stress treatments. Interestingly, Tre application enhanced the SOD activity in all the stress treatments but inhibited the POD activity. Both CAT and GPX activity were increased by saline and low P stress while the activities inhibited in combined stress. Similar results were found for APX, GR, and DHAR activities in both genotypes. However, MDHAR activity was inhibited in all the stresses. Interestingly, Tre enhanced CAT APX, GPX, GR, MDHAR and DHAR activities suggesting the amelioration of ROS scavenging in maize under all the stresses. Increased GST activity in presence or absence of Tre might involve in detoxification of hydroperoxides as well as leaf senescence. On the other hand, increased glyoxalase activities in saline and low P stress in BHM-9 suggested better MG detoxification system because of down-regulation of Gly-I activity in BHM-7 in those stresses. Tre also increased the glyoxalase activities in both genotypes under all the stresses. Tre improved the growth in maize seedlings by decreasing Na+/K+, ROS, MDA, and MG through regulating antioxidant and glyoxalase systems.

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Impact of abiotic stressors on native rhizospheric bacterial community of Cajanus cajan

2019

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Salinity and drought are the major abiotic stresses that limit agricultural productivity. Application of plant growth promoting rhizobacteria (PGPR) is an attractive technology but with the bottlenecks of reduced efficacy and survivability in the environment. For increased efficiency of PGPR strains, the impact of stresses on the native bacterial community needs to be studied. Experimentally induced stresses would be ideal to assess the immediate perturbances in the structure of soil bacterial community. Hence, the study focused on the effect of experimentally‐induced salinity, and drought stress on rhizospheric bacterial community of Cajanus cajan. A plant growth experiment was set up to induce salinity and drought stresses. Shifts in the bacterial community were assessed by a culture‐independent technique of denaturing gradient gel electrophoresis using 16S ribosomal RNA gene and transcript as markers, leading to a comparison of the resident with the active bacterial community. The impact on plant was evaluated by measurement of plant biometrics. Further, salinity and drought‐stressed conditions led to distinct shifts in native and active rhizospheric bacterial community, corresponding to the higher decline at induction of stresses, and stabilization at later time points. The study encompasses the perturbations in the active and resident rhizospheric bacterial community caused by the induction of two different abiotic stresses along the plant's growth.

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Mitigation of PEG-induced drought stress in rapeseed (Brassica rapa L.) by exogenous application of osmolytes

Cited by 50 publications

References 40 publications

Trehalose Protects Maize Plants from Salt Stress and Phosphorus Deficiency

Trehalose Protects Maize Plants from Salt Stress and Phosphorus Deficiency

Trehalose Protects Maize Plants from Salt Stress and Phosphorus Deficiency

Impact of abiotic stressors on native rhizospheric bacterial community of Cajanus cajan

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