2021
DOI: 10.3390/antiox10121954
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Mito-TIPTP Increases Mitochondrial Function by Repressing the Rubicon-p22phox Interaction in Colitis-Induced Mice

Abstract: The run/cysteine-rich-domain-containing Beclin1-interacting autophagy protein (Rubicon) is essential for the regulation of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase by interacting with p22phox to trigger the production of reactive oxygen species (ROS) in immune cells. In a previous study, we demonstrated that the interaction of Rubicon with p22phox increases cellular ROS levels. The correlation between Rubicon and mitochondrial ROS (mtROS) is poorly understood. Here, we report that Rubicon in… Show more

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Cited by 6 publications
(4 citation statements)
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“…A DSS-induced colitis mouse model was developed using 6-week-old female C57BL/6 mice (Koatech, Pyeongtaek, Korea), as described previously 89 . Colitis was induced using 2.5% DSS (molecular weight: 36,000-50,000 kDa, MP Biomedicals, Santa Ana, CA, USA) for five days, followed by water for two days (i.e., a total of seven days).…”
Section: Determination Of Survival Rate and Dai Using A Dss-induced C...mentioning
confidence: 99%
“…A DSS-induced colitis mouse model was developed using 6-week-old female C57BL/6 mice (Koatech, Pyeongtaek, Korea), as described previously 89 . Colitis was induced using 2.5% DSS (molecular weight: 36,000-50,000 kDa, MP Biomedicals, Santa Ana, CA, USA) for five days, followed by water for two days (i.e., a total of seven days).…”
Section: Determination Of Survival Rate and Dai Using A Dss-induced C...mentioning
confidence: 99%
“…Interestingly, treatment with the antioxidant N-acetylcysteine ameliorated the signs of colitis, apoptosis and oxidative stress, highlighting the important role of ROS in the pathophysiology of IBD. A study of Rubicon further discusses the role of mitochondrial ROS in IBD [25]. Rubicon is a positive regulator of the NADPH oxidase complex and it is involved in autophagy and inflammatory responses.…”
Section: Mitochondrial Ros and Oxidative Stressmentioning
confidence: 99%
“…As previously described, DSS-induced acute or chronic colitis mouse models were developed using 6-week-old C57BL/6 female mice (Samtako, Osan, Republic of Korea) [ 36 ]. To assess the trigger of acute colitis, mice were treated with 3% ( w / v ) dextran sodium sulfate (molecular weight: 36,000–50,000 kDa, MP Biomedicals, Santa Ana, CA, USA) dissolved in drinking water that was given ad libitum.…”
Section: Materials and Methdosmentioning
confidence: 99%
“…Four-millimeter paraffin slices were cut and stained with hematoxylin and eosin (H&E). As previously mentioned, a board-certified pathologist (Dr. Min-Kyung Kim, Kim Min-Kyung Pathology Clinic, Seoul, Korea) independently scored each organ segment without prior knowledge of the therapy groups [ 36 ].…”
Section: Materials and Methdosmentioning
confidence: 99%