Mitochondria from ejaculated human spermatozoa do not synthesize proteins

Dı́ez-Sánchez, Carmen; Ruiz‐Pesini, Eduardo; Montoya, Julio; Pérez‐Martos, Acisclo; Enrı́quez, José Antonio; López‐Pérez, Manuel J.

doi:10.1016/s0014-5793(03)01013-5

Cited by 28 publications

(19 citation statements)

References 21 publications

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“…The mammalian spermatozoa must be progressively motile for a certain period of time to fertilize an oocyte (Mukai & Okuno, 2004). It has been proposed that the energy required to regulate sperm motility is provided by ATP, which is predominantly produced via mitochondrial respiration (D ıez-S anchez et al, 2003;Miki et al, 2004;Mukai & Okuno, 2004). Therefore, if mitochondrial respiration is unable to synthesize ATP, it could decrease the sperm motility.…”

Section: Discussionmentioning

confidence: 99%

Sodium nitroprusside suppresses male fertility in vitro

et al. 2014

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SUMMARYSodium nitroprusside is a nitric oxide donor involved in the regulation of the motility, hyperactivation, capacitation, and acrosome reaction (AR) of spermatozoa. However, the molecular mechanism underlying this regulation has not yet been elucidated. Therefore, this study was designed to evaluate the molecular basis for the effects of sodium nitroprusside on different processes in spermatozoa and its consequences on subsequent oocyte fertilization and embryo development. In this in vitro study, mouse spermatozoa were incubated with various concentrations of sodium nitroprusside (1, 10, and 100 lM) for 90 min. Our results showed that sodium nitroprusside inhibited sperm motility and motion kinematics in a dose-dependent manner by significantly enhancing intracellular iron and reactive oxygen species (ROS), and decreasing Ca 2+ , and adenosine triphosphate levels in spermatozoa. Moreover, short-term exposure of spermatozoa to sodium nitroprusside increased the tyrosine phosphorylation of sperm proteins involved in PKA-dependent regulation of intracellular calcium levels, which induced a robust AR. Finally, sodium nitroprusside significantly decreased the rates of fertilization and blastocyst formation during embryo development. Based on these results, we propose that sodium nitroprusside increases ROS production and precocious AR may alter overall sperm physiology, leading to poor fertilization and compromised embryonic development.

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Section: Discussionmentioning

confidence: 99%

Sodium nitroprusside suppresses male fertility in vitro

et al. 2014

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“…Because mature mammalian spermatozoa have stopped protein synthesis (27), this analysis cannot be performed at the mRNA level (e.g. by reverse transcription-PCR or in situ hybridization).…”

Section: Expression Of Hor17-4 Stimulatory G-proteins and Mac(s)-mentioning

confidence: 99%

Particulate Adenylate Cyclase Plays a Key Role in Human Sperm Olfactory Receptor-mediated Chemotaxis

Spehr

Schwane

Riffell

et al. 2004

Journal of Biological Chemistry

142

131

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Human sperm chemotaxis is a critical component of the fertilization process, but the molecular basis for this behavior remains unclear. Recent evidence shows that chemotactic responses depend on activation of the sperm olfactory receptor, hOR17-4. Certain floral scents, including bourgeonal, activate hOR17-4, trigger pronounced Ca 2؉ fluxes, and evoke chemotaxis. Here, we provide evidence that hOR17-4 activation is coupled to a cAMP-mediated signaling cascade. Multidimensional protein identification technology was used to identify potential components of a G-protein-coupled cAMP transduction pathway in human sperm. These products included various membrane-associated adenylate cyclase (mAC) isoforms and the G olf -subunit. Using immunocytochemistry, specific mAC isoforms were localized to particular cell regions. Whereas mAC III occurred in the sperm head and midpiece, mAC VIII was distributed predominantly in the flagellum. In contrast, G olf was found mostly in the flagellum and midpiece. The observed spatial distribution patterns largely correspond to the spatiotemporal character of hOR17-4-induced Ca 2؉ changes. Behavioral and Ca 2؉ signaling responses of human sperm to bourgeonal were bioassayed in the presence, or absence, of the adenylate cyclase antagonist SQ22536. This specific agent inhibits particulate AC, but not soluble AC, activation. Upon incubation with SQ22536, cells ceased to exhibit Ca 2؉ signaling, chemotaxis, and hyperactivation (faster swim speed and flagellar beat rate) in response to bourgeonal. Particulate AC is therefore required for induction of hOR17-4-mediated human sperm behavior and represents a promising target for future design of contraceptive drugs.

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“…The question, therefore, of whether sperm has a translational capacity that cannot be ascribed to another contaminating cell type (including contaminating bacteria) remains open. De novo, chloramphenicol-(CP-) sensitive, mitochondrially directed protein synthesis in capacitating human sperm was recently demonstrated using the uptake of 35 S-methionine-lysine and BODIPY lysine tRNA [47] This report did not cite a study appearing three years earlier, concluding that protein synthesis in sperm was due to CP-sensitive bacterial contamination (repeating an assertion made almost 40 years previously; [53,54]). The only other report of de novo protein synthesis in sperm used a 2D PAGE based proteomic approach to identify 44 differentially expressed proteins in capacitated versus noncapacitated sperm including sperm capacitated in the presence of chloramphenicol [47].…”

Section: Historical Contextmentioning

confidence: 92%

“…Between them, MacLaughlin and Terner [66] and Premkumar and Bhargava [67] demonstrated that mature bovine spermatozoa have no nuclear transcription to speak of (as might be expected) but that mitochondrial RNA was actively transcribed. Translation of (mitochondrial) RNAs was inferred rather than demonstrated at the time although at least one more recent report suggested that even mitochondrial gene expression is also absent in mature sperm [54].…”

Section: Historical Contextmentioning

confidence: 99%

“…In this regard, although a relationship between extra-mitochondrial mitochondrial ribosomes and germ cell formation has been reported in insects and fish [52], how such a mechanism can support the translation of cytoplasmic RNAs (including nuclear-encoded RNAs for mitochondrial proteins) demands explanation. In a cautionary context, other reports have suggested that the reported translational activity in ejaculate sperm (even mitochondrially driven) could also be an artifact of bacterial contamination [53,54]. The more recent of these reports, however, while implicating bacteria fell short of actually demonstrating their presence in semen samples concerned.…”

Section: Introductionmentioning

confidence: 96%

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Sperm RNA as a Mediator of Genomic Plasticity

Miller

2014

Advances in Biology

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Sperm RNA has been linked recently to trans-generational, non-Mendelian patterns of inheritance. Originally dismissed as "residual" to spermatogenesis, some sperm RNA may have postfertilization functions including the transmission of acquired characteristics. Sperm RNA may help explain how trans-generational effects are transmitted and it may also have implications for assisted reproductive technologies (ART) where sperm are subjected to considerable, ex vivo manual handling. The presence of sperm RNA was originally a controversial topic because nuclear gene expression is switched off in the mature mammalian spermatozoon. With the recent application of next generation sequencing (NGS), an unexpectedly rich and complex repertoire of RNAs has been revealed in the sperm of several species that makes its residual presence counterintuitive. What follows is a personal survey of the science behind our understanding of sperm RNA and its functional significance based on experimental observations from my laboratory as well as many others who have contributed to the field over the years and are continuing to contribute today. The narrative begins with a historical perspective and ends with some educated speculation on where research into sperm RNA is likely to lead us in the next 10 years or so.

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Mitochondria from ejaculated human spermatozoa do not synthesize proteins

Cited by 28 publications

References 21 publications

Sodium nitroprusside suppresses male fertility in vitro

Sodium nitroprusside suppresses male fertility in vitro

Particulate Adenylate Cyclase Plays a Key Role in Human Sperm Olfactory Receptor-mediated Chemotaxis

Sperm RNA as a Mediator of Genomic Plasticity

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