Mitochondria in the midgut epithelial cells of sugarcane borer parasitized by Cotesia flavipes (Cameron, 1891)

Abstract

The sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae) has been controlled by Cotesia flavipes (Hymenoptera: Braconidae); however, very little is known about the effect of the parasitism in the host organs, including the midgut. This work aims to verify mitochondrial alteration in the different midgut epithelial cells of D. saccharalis parasitized by C. flavipes. Midgut fragments (anterior and posterior region) of both non-parasitized and parasitized larvae were processed for transmission electron microscopy. The mitochondria of midgut epithelial cell in the parasitized larvae exhibit morphological alteration, represented by matrix rarefaction and vacuolisation. These mitochondrial alterations are more pronounced in the anterior midgut region during the parasitism process, mainly in the columnar cell.Keywords: midgut, Diatraea saccharalis, mitochondria, Cotesia flavipes, epithelial cell. Mitocôndria nas Células Epiteliais do Intestino Médio da Broca-da-cana Parasitada por Cotesia flavipes ResumoDiatraea saccharalis (Lepidoptera: Crambidae), broca da cana-de-açúcar, tem sido controlada por Cotesia flavipes (Hymenoptera: Braconidae); pouco se sabe sobre o efeito do parasitismo nos diferentes órgãos do inseto hospedeiro, principalmente no intestino médio. O objetivo desse trabalho foi verificar as alterações mitocondriais das diferentes células epiteliais do intestino médio de larvas de D. saccharalis parasitadas por C. flavipes. Fragmentos do intestino médio (regiões anterior e posterior) de larvas de D. saccharalis não-parasitadas e parasitadas foram processados para microscopia eletrônica de transmissão. As mitocôndrias das células epiteliais do intestino médio de larvas parasitadas exibem alterações, especialmente rarefação e vacuolização da matriz, que foram mais pronunciadas nas células epiteliais da região anterior do intestino médio na vigência do parasitismo, em especial nas células colunares.Palavras-chave: intestino médio, Diatraea saccharalis, mitocôndria, Cotesia flavipes, célula epitelial.

Investigation of midgut's ultrastructure of Notonecta viridis Decourt, 1909 and Notonecta maculata Fab., 1794 (Hemiptera:Notonectidae)

“…As stated in many studies [7][8][9][10][11][12], we have observed significantly larger nucleus, nucleolus and the RER cisternae in the cytoplasm of the midgut cells of both species.…”

“…There are more secretory granules in endocrine cells than in other cells [6,12,14,21]. It suggests that these cells are specialized cells to make secretion.…”

Malpighian Tubules in Larvae of Diatraea saccharalis (Lepidoptera; Crambidae): A Morphological Comparison between Non-Parasitized and Parasitized by Cotesia flavipes (Hymenoptera; Braconidae)

“…These polymorphic, electron-dense organelles were observed in larval cells up to day 5 of parasitism. In some cells, mitochondria showed vacuoles and loss of the matrix, which indicated degeneration, as described by [20] for the mid-intestinal cells of D. saccharalis parasitized by the same parasitoid.…”

Effects of neem oil (Azadirachta indica A. Juss) on midgut cells of predatory larvae Ceraeochrysa claveri (Navás, 1911) (Neuroptera: Chrysopidae)

“…Spherites or spherocrystals are associated with absorption, transport and storage of organic and inorganic elements, which could be utilized on demand for metabolic process in the midgut epithelial cells as observed in D. saccharalis by Pinheiro et al (2008), in Brontocoris tabidus (Heteroptera: Pentatomidae) by Fialho et al (2009), in Cimex hemipterus (Hemiptera: Cimicidae) by Azevedo et al (2009), in Triatoma vitticeps (Hemiptera: Reduviidae) by Rocha et al (2010) and Euroleon nostras (Neuroptera: Myrmeleontidae) by Lipovšek et al (2012).…”