Mitochondria inheritance is a key factor for tolerance to dehydration in wine yeast production

Picazo, Cecilia; Gamero-Sandemetrio, Esther; Orozco, Helena; Albertin, Warren; Marullo, Philippe; Matallana, Emilia; Aranda, Agustı́n

doi:10.1111/lam.12369

Cited by 21 publications

(11 citation statements)

References 20 publications

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“…Has adaptation played a role in driving these incompatibilities? Although no direct links are proven, evolution of the mitochondrial genome and mito-nuclear epistasis has been linked to multiple phenotypes (Solieri et al 2008;Albertin et al 2013;Picazo et al 2014), including 37°C growth (Paliwal et al 2014, Wolters et al 2018, Leducq et al 2017, and deficiencies in mitochondrial DNA cause heat sensitivity (Zubko and Zubko 2014). Here, we show that mtDNA is important for evolution of heat and cold tolerance in distantly related species, caused by the accumulation of multiple small-to-medium effect changes and potentially mito-nuclear epistasis.…”

Section: Mitochondrial Dna and Yeast Evolutionmentioning

confidence: 79%

Mitochondria-encoded genes contribute to the evolution of heat and cold tolerance amongSaccharomycesspecies

Peris

et al. 2018

Preprint

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Over time, species evolve substantial phenotype differences. Yet, genetic analysis of these traits is limited by reproductive barriers to those phenotypes that distinguish closely related species. Here, we conduct a genome-wide non-complementation screen to identify genes that contribute to a major difference in thermal growth profile between two Saccharomyces species. S. cerevisiae is capable of growing at temperatures exceeding 40C, whereas S. uvarum cannot grow above 33C but outperforms S. cerevisiae at 4C. The screen revealed only a single nuclear-encoded gene with a modest contribution to heat tolerance, but a large effect of the species' mitochondrial DNA (mitotype). Furthermore, we found that, while the S. cerevisiae mitotype confers heat tolerance, the S. uvarum mitotype confers cold tolerance. Recombinant mitotypes indicate multiple genes contribute to thermal divergence. Mitochondrial allele replacements showed that divergence in the coding sequence of COX1 has a moderate effect on both heat and cold tolerance, but it does not explain the entire difference between the two mitochondrial genomes. Our results highlight a polygenic architecture for interspecific phenotypic divergence and point to the mitochondrial genome as an evolutionary hotspot for not only reproductive incompatibilities, but also thermal divergence in yeast.

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Section: Mitochondrial Dna and Yeast Evolutionmentioning

confidence: 79%

Mitochondria-encoded genes contribute to the evolution of heat and cold tolerance amongSaccharomycesspecies

Peris

et al. 2018

Preprint

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“…Different stress tolerances exist, even among Saccharomyces species of enological interest. Using hybrids, it has been proven that S. cerevisiae mitochondria better protect against dehydration than Saccharomyces uvarum mitochondria (Picazo et al 2015a). Therefore, the oxidative stress response is a potential target for wine yeast improvement, and using natural antioxidants like argan oil is a feasible way to improve biomass production (Gamero-Sandemetrio et al 2015).…”

Section: Ady Productionmentioning

confidence: 99%

Biotechnological impact of stress response on wine yeast

Matallana

Aranda

2016

Lett Appl Microbiol

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Wine yeast deals with many stress conditions during its biotechnological use. Biomass production and its dehydration produce major oxidative stress, while hyperosmotic shock, ethanol toxicity and starvation are relevant during grape juice fermentation. Most stress response mechanisms described in laboratory strains of Saccharomyces cerevisiae are useful for understanding the molecular machinery devoted to deal with harsh conditions during industrial wine yeast uses. However, the particularities of these strains themselves, and the media and conditions employed, need to be specifically looked at when studying protection mechanisms.

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“…The results showed that hybrid strain clones containing mtDNA of S. cerevisiae were more resistant to drying procedures than the clones containing S. uvarum mtDNA. The authors of this research concluded that the resistance of mtDNA to water loss is a very important factor in the survival of dehydrated yeast cells [37]. This study showed the importance of mitochondria and their role in the maintenance of cell viability during yeast dehydration, storage in the dry state, and rehydration/reactivation.…”

Section: Introductionmentioning

confidence: 61%

Anhydrobiosis in Yeasts: Changes in Mitochondrial Membranes Improve the Resistance of Saccharomyces cerevisiae Cells to Dehydration–Rehydration

Khroustalyova

Rapoport

2019

Fermentation

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Anhydrobiosis is a unique state of live organisms in which their metabolism is temporary reversibly suspended as the result of strong dehydration of their cells. This state is widely used currently during large-capacity production of active dry baker's yeast. Other strains of the yeast Saccharomyces cerevisiae, as well as other yeast species that could potentially find use in modern biotechnology, are not resistant to dehydration-rehydration treatments. To improve their resistance, the main factors that influence cell survival during such treatment need to be revealed. This study showed the importance of mitochondria for yeast cell survival during transfer into anhydrobiosis, a factor that was strongly underestimated until this study. It was revealed that the external introduction inside yeast cells of 50 µM of lithocholic acid (LCA), an agent that induces changes in glycerophospholipids in mitochondrial membranes, in combination with 1% DMSO, may improve the survival rate of dehydrated cells. The influence of LCA upon yeast cell resistance to dehydrationrehydration was not linked with changes in the state of the cells' plasma membrane.

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Mitochondria inheritance is a key factor for tolerance to dehydration in wine yeast production

Cited by 21 publications

References 20 publications

Mitochondria-encoded genes contribute to the evolution of heat and cold tolerance amongSaccharomycesspecies

Mitochondria-encoded genes contribute to the evolution of heat and cold tolerance amongSaccharomycesspecies

Biotechnological impact of stress response on wine yeast

Anhydrobiosis in Yeasts: Changes in Mitochondrial Membranes Improve the Resistance of Saccharomyces cerevisiae Cells to Dehydration–Rehydration

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