Mitochondria Mediate Amplification of Luteinizing Hormone Action by Adenosine in Luteal Cells*

Soodak, Lenore K.; Behrman, Harold R.

doi:10.1210/endo-122-4-1308

Cited by 7 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The females of the three age groups were examined at baseline, and were given an ovarian challenge with pregnant mare serum gonadotropin (PMSG) to replicate preovulatory conditions, so as to further distinguish the differences in the three age groups. Therefore, the rats were sacrificed at 0 hours and at 54 hours, after an injection of PMSG (1 IU/g body weight; Sigma, St. Louis, MO) (32). Blood was collected via terminal cardiac puncture.…”

mentioning

confidence: 99%

Serum and ovarian Müllerian inhibiting substance, and their decline in reproductive aging

Yeh

Kim

Peresie

et al. 2007

Fertility and Sterility

View full text Add to dashboard Cite

mentioning

confidence: 99%

Serum and ovarian Müllerian inhibiting substance, and their decline in reproductive aging

Yeh

Kim

Peresie

et al. 2007

Fertility and Sterility

View full text Add to dashboard Cite

“…Adenosine produces a large increase in luteal cell ATP content (11,19). Also, mitochondrial oxidative phosphorylation and ATP production are necessary for amplification by adenosine of LH-stimulated cAMP accumulation (3). Thus, it is possible that the increase in cellular ATP results in increased cAMP accumulation due to increased substrate availability.…”

Section: Discussionmentioning

confidence: 99%

“…The decrease in cellular ATP levels was not unexpected, because it is well known that increasing cellular calcium levels cause increased ATP utilization and decreased ATP production (23,24). Since luteal cell ATP content is an important factor in the regulation of LH-stimulated cAMP production (3,11), it was important to determine whether the effect of ionomycin on luteal cell cAMP accumulation was due to increased cellular calcium levels or to decreased cellular ATP levels. Cholera toxin-stimulated cAMP and ATP levels were both decreased by ionomycin.…”

Section: Discussionmentioning

confidence: 99%

“…This effect of adenosine is defined by an increase in maximal LH-stimulated cAMP accumulation up to 10-fold or more and a decrease in the ED 50 of LH for progesterone production by a similar magnitude (1). The progonadotropic action of adenosine in luteal cells occurs at an intracellular site, requires an intact cell (1), and is dependent on oxidative-phosphorylation and ATP production (3). However, the mechanism of ATP-dependent amplification of the action of LH by adenosine is not known.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Selective Amplification of Luteinizing Hormone by Adenosine in Rat Luteal Cells*

1988

Self Cite

View full text Add to dashboard Cite

Adenosine amplification of LH-stimulated cAMP accumulation in rat luteal cells is rapid and dependent on mitochondrial ATP production. The objective of the present studies was to determine if this effect of adenosine is specific for LH and to gain information on the mechanism of the ATP-dependent amplification of LH action in rat luteal cells. Adenosine significantly amplified maximum cAMP accumulation in response to LH, isoproterenol, forskolin, and cholera toxin. However, amplification of this response by adenosine was significantly greater for LH than for the other agonists. The relative order of amplification by adenosine was LH greater than isoproterenol greater than forskolin greater than cholera toxin; the relative magnitudes of amplification by adenosine were 1, 0.6, 0.2, and 0.2, respectively. Neither LH, isoproterenol, forskolin, nor cholera toxin had any effect on cellular levels of ATP, and adenosine produced a similar rate of increase and maximal levels of ATP in the presence of all agonists. Ionomycin, a calcium ionophore, inhibited LH- and cholera toxin-stimulated cAMP accumulation and produced a dose-dependent depletion of ATP. Adenosine reversed the inhibitory effect of ionomycin on LH-stimulated cAMP accumulation and cellular levels of ATP. However, adenosine did not reverse the inhibitory effect of ionomycin on cholera toxin-stimulated cAMP accumulation, although its effects on cellular ATP levels were identical to those on LH. Thus, the selective amplification of LH by adenosine is not merely a substrate effect on adenylate cyclase activity. The nature of adenylate cyclase activation by cholera toxin and forskolin and the weak amplification by adenosine of these agonists compared to that of LH indicate that the site of the ATP-dependent action of adenosine appears to be before or on the G-protein of adenylate cyclase. We suggest that adenosine, by an ATP-dependent process, either increases the availability of functional LH receptors or increases coupling between the LH receptor and adenylate cyclase.

show abstract

“…The mixture was centrifuged for 10 min (4°C, 2000 g). Following centrifugation, the supernatant was mixed with two volumes of tri-n-octylamine-freon (0.22 M tri-n-octylamine), centrifuged (4°C, 2000 g) and the aqueous layer collected and stored at -75 °C as described (Soodak et al, 1988).…”

Section: Preparation Of Samplesmentioning

confidence: 99%

Purine levels and metabolism in human follicular fluid*

1990

Self Cite

View full text Add to dashboard Cite

Adenosine (ADO) in low micromolar levels and hypoxanthine (HYP) in millimolar levels have been shown to inhibit maturation of cumulus-enclosed oocytes. To determine the effect of ovarian stimulation with gonadotrophins on follicular purine metabolism, we measured ADO, HYP, inosine (INO), adenine (ADE) and cyclic AMP (cAMP) levels in follicular fluid (FF) from natural (n = 7) or human menopausal gonadotrophin/human chorionic gonadotrophin (HMG/HCG)-stimulated (n = 35) cycles. Purines were extracted immediately (natural cycles) or within 30 min of recovery (HMG/HCG cycles) and analysed by high pressure liquid chromatography (HPLC). The concentration of all ADE purines in FF was in the low micromolar range (1-20 microM); cAMP levels were markedly increased (greater than 100 microM) in FF of HMG/HCG-treated patients. While ADO levels were within the range effective for inhibition of oocyte maturation, those of HYP were not. No correlation was found between purine levels in FF and ovum maturation. Purine levels in FF of natural cycles were uniformly lower than those of stimulated cycles. Significant conversion of 5'-AMP into ADO, ADO into INO and INO into HYP occurred within 1 h when FF was incubated at 25 but not at 4 degrees C. These purine levels in human FF confirm our previous findings with bovine FF and suggest a possible role of ADO, but not of HYP, in the inhibition of oocyte maturation in the human.

show abstract

Mitochondria Mediate Amplification of Luteinizing Hormone Action by Adenosine in Luteal Cells*

Cited by 7 publications

References 0 publications

Serum and ovarian Müllerian inhibiting substance, and their decline in reproductive aging

Serum and ovarian Müllerian inhibiting substance, and their decline in reproductive aging

Selective Amplification of Luteinizing Hormone by Adenosine in Rat Luteal Cells*

Purine levels and metabolism in human follicular fluid*

Contact Info

Product

Resources

About