Mitochondria-rich cell activity in the yolk-sac membrane of tilapia(<i>Oreochromis mossambicus</i>) larvae acclimatized to different ambient chloride levels

Li, Lin; Hwang, Pung Pung

doi:10.1242/jeb.00869

Cited by 39 publications

(26 citation statements)

References 35 publications

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“…By contrast, convex apical surfaces were prominent in the LowCl group, suggesting that convex apical surfaces are involved in Cl -uptake. The convex apical surface is considered to be identical to the wavy-convex structure, previously reported in tilapia (Lee et al, 1996;Chang et al, 2001;Chang et al, 2003;Lin and Hwang, 2004) and goldfish (Chang et al, 2002). The apical size and relative density of wavy-convex MR cells were found to increase in low Cl -conditions and to be positively correlated with Cl -influx .…”

Section: Discussionmentioning

confidence: 94%

“…The apical size and relative density of wavy-convex MR cells were found to increase in low Cl -conditions and to be positively correlated with Cl -influx . Since MR cells are in contact with ambient water via their apical surface, the appearance of wavy-convex MR cells with larger apical surface was considered to enhance Cl -uptake Lin and Hwang, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Half the water was changed every other day to guarantee optimal water quality. Artificial freshwaters were prepared by dissolving appropriate amounts of NaCl, Na 2 SO 4 , MgSO 4 , K 2 HPO 4 , KH 2 PO 4 , CaCl 2 and CaSO 4 in deionized water, according to the method described in previous studies (Chang et al, 2001;Chang et al, 2002;Chang et al, 2003;Lin and Hwang, 2004). The pH of the media was between 6.65 and 6.75.…”

Section: Materials and Methods Fishmentioning

confidence: 99%

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Morphological and functional classification of ion-absorbing mitochondria-rich cells in the gills of Mozambique tilapia

Inokuchi

Hiroi

Watanabe

et al. 2009

Journal of Experimental Biology

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SUMMARY To clarify ion-absorbing functions and molecular mechanisms of mitochondria-rich (MR) cells, Mozambique tilapia (Oreochromis mossambicus) were acclimated to artificial freshwaters with normal or lowered Na+ and/or Cl– concentration: (1) normal Na+/normal Cl– (control); (2) normal Na+/low Cl–; (3) low Na+/normal Cl–; and (4) low Na+/low Cl–. Scanning electron microscopy (SEM) revealed that concave and convex apical surfaces of MR cells predominantly developed in low Na+ and low Cl– waters, respectively, whereas small apical pits predominated in control conditions. Expression of Na+/H+exchanger-3 (NHE3) mRNA in the gills was increased in low Na+waters (low Na+/normal Cl– and low Na+/low Cl–), whereas that of Na+/Cl– cotransporter (NCC) expression was upregulated in low Cl–, but not in low Na+/low Cl–. Immunofluorescence staining showed that enlarged NHE3-immunoreactive apical regions were concave or flat in low Na+waters, whereas NCC-immunoreactive regions were enlarged convexly in low Cl– waters. Using SEM immunocytochemistry the distribution of NHE3/NCC was compared with SEM images obtained simultaneously, it was further demonstrated that NHE3 and NCC were confined to concave and convex apical surfaces, respectively. These results indicated that small apical pits developed into concave apical surfaces to facilitate Na+ uptake through NHE3, and into convex apical surfaces to enhance Na+/Cl– uptake through NCC. Our findings integrated morphological and functional classifications of ion-absorbing MR cells in Mozambique tilapia.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Materials and Methods Fishmentioning

confidence: 99%

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Morphological and functional classification of ion-absorbing mitochondria-rich cells in the gills of Mozambique tilapia

Inokuchi

Hiroi

Watanabe

et al. 2009

Journal of Experimental Biology

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“…Therefore, tilapia and killifish, the two typical experimental models for euryhaline fish, seem to share a similar on-off mechanism of active ion secretion, which is achieved by the rapid appearance and disappearance of CFTR at the apical membrane of MRCs. It has also been reported that MRCs close the apical openings quickly (about 30·min or a few hours, which seem likely to be quicker than the response of CFTR) in response to abrupt salinity changes or acid-base disturbances (Goss et al, 1995;Sakamoto et al, 2000;Daborn et al, 2001;Lin and Hwang, 2004). Although we confirmed in DIC observations that all of the type-III cells that appeared following transfer from seawater to freshwater possessed a distinct apical opening, it is possible that the apical opening of the MRCs was closed within 12·h, in advance of the disappearance of the apical CFTR distribution.…”

Section: Seawater-type Ion Secretory Cellmentioning

confidence: 95%

Functional classification of mitochondrion-rich cells in euryhaline Mozambique tilapia (Oreochromis mossambicus) embryos, by means of triple immunofluorescence staining for Na+/K+-ATPase,Na+/K+/2Cl- cotransporter and CFTR anion channel

Hiroi

McCormick

Ohtani-Kaneko

et al. 2005

Journal of Experimental Biology

197

154

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SUMMARY Mozambique tilapia Oreochromis mossambicus embryos were transferred from freshwater to seawater and vice versa, and short-term changes in the localization of three major ion transport proteins,Na+/K+-ATPase,Na+/K+/2Cl- cotransporter (NKCC) and cystic fibrosis transmembrane conductance regulator (CFTR) were examined within mitochondrion-rich cells (MRCs) in the embryonic yolk-sac membrane. Triple-color immunofluorescence staining allowed us to classify MRCs into four types: type I, showing only basolateral Na+/K+-ATPase staining; type II, basolateral Na+/K+-ATPase and apical NKCC; type III, basolateral Na+/K+-ATPase and basolateral NKCC; type IV, basolateral Na+/K+-ATPase,basolateral NKCC and apical CFTR. In freshwater, type-I, type-II and type-III cells were observed. Following transfer from freshwater to seawater, type-IV cells appeared at 12 h and showed a remarkable increase in number between 24 h and 48 h, whereas type-III cells disappeared. When transferred from seawater back to freshwater, type-IV cells decreased and disappeared at 48 h, type-III cells increased, and type-II cells, which were not found in seawater, appeared at 12 h and increased in number thereafter. Type-I cells existed consistently irrespective of salinity changes. These results suggest that type I is an immature MRC, type II is a freshwater-type ion absorptive cell, type III is a dormant type-IV cell and/or an ion absorptive cell (with a different mechanism from type II), and type IV is a seawater-type ion secretory cell. The intracellular localization of the three ion transport proteins in type-IV cells is completely consistent with a widely accepted model for ion secretion by MRCs. A new model for ion absorption is proposed based on type-II cells possessing apical NKCC.

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“…For both species, a specific mitochondrial dye was used to distinguish between cells from Fractions I and II (Lin and Hwang 2004;Lin et al 2006;Buhariwalla et al 2012). The fluorescent dye DASPEI (2-(4-dimethylaminostyryl)-1-methylpyridinium iodide; Sigma-Aldrich, St. Louis, MO, USA) was used in isolated gill cells of L. costata.…”

Section: Cell Distinctionmentioning

confidence: 99%

Isolation and fractionation of gill cells from freshwater (Lasmigona costata) and seawater (Mesodesma mactroides) bivalves for use in toxicological studies with copper

Nogueira¹,

Wood

Gillis

et al. 2013

Cytotechnology

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Gills cells of the freshwater mussel Lasmigona costata and the seawater clam Mesodesma mactroides were isolated (mussel: chemical dissociation; clam: mechanical dissociation) and fractionated (Percoll gradient) into Fractions I and II. Mitochondrial dyes (DASPEI: mussel; MitoTracker Ò : clam) and Na ? , K ? -ATPase activity measurement were used to distinguish between cells of Fractions I and II. For mussel and clam, 80.5 ± 1.5 and 48.3 ± 3.2 % of cells were in Fraction II, respectively. For both species, cells of Fraction II had higher fluorescence emission and higher enzyme activity than those of Fraction I, being characterized as 'cells rich in mitochondria'. Cells of Fraction II were kept in saline solutions approximating the ionic composition of hemolymph either under control conditions (no Cu addition) or exposed (3 h) to copper (Cu: 5, 9 and 20 lg Cu/L). Cell viability and Cu and Na ? content were measured. For both species, Cu content was higher and Na ? content was lower in cells exposed to 20 lg Cu/L. Furthermore, a strong negative correlation was observed between cell Na ? and Cu content in the two bivalve species, indicating a possible competition between Cu and Na ? for ion-transporting mechanisms or binding sites at gill cells of Fraction II. Considering that Cu is an ionoregulatory toxicant in aquatic invertebrates, these preliminary toxicological data support the idea of using isolated gill cells rich in mitochondria to study the mechanisms underlying the acute toxicity of waterborne Cu in freshwater and marine bivalves.

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Mitochondria-rich cell activity in the yolk-sac membrane of tilapia(Oreochromis mossambicus) larvae acclimatized to different ambient chloride levels

Abstract: Mitochondria-rich cells (MRCs

Cited by 39 publications

References 35 publications

Morphological and functional classification of ion-absorbing mitochondria-rich cells in the gills of Mozambique tilapia

Morphological and functional classification of ion-absorbing mitochondria-rich cells in the gills of Mozambique tilapia

Functional classification of mitochondrion-rich cells in euryhaline Mozambique tilapia (Oreochromis mossambicus) embryos, by means of triple immunofluorescence staining for Na+/K+-ATPase,Na+/K+/2Cl- cotransporter and CFTR anion channel

Isolation and fractionation of gill cells from freshwater (Lasmigona costata) and seawater (Mesodesma mactroides) bivalves for use in toxicological studies with copper

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