Mitochondrial and nuclear intraspecific variation in the rusty blenny (Parablennius sanguinolentus, Blenniidae)

Pappalardo, Andrea; Francisco, Sara Martins; Fruciano, Carmelo; Lima, Cristina S.; Pulvirenti, Valentina; Tigano, Concetta; Robalo, Joana Isabel; Ferrito, Venera

doi:10.1007/s10750-017-3248-6

Cited by 6 publications

(4 citation statements)

References 76 publications

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“…While the present study used only one geographical location and two genetic markers, these apparent limitations allowed a rigorous comparison with data from several species collected in the same area over the past fifteen years and using the same two markers (mitochondrial control region and nuclear S7) (e.g., Domingues et al, 2006;Almada et al, 2012;Robalo et al, 2013;Francisco et al, 2014;Stefanni et al, 2015;Almada et al, 2017;Pappalardo et al, 2017), which facilitated comparison and calibration of genetic diversity results. Thus, it was possible to observe the high genetic diversity pattern found in several coastal fish populations in western Portugal (e.g., D. vulgaris (Stefanni et al, 2015) and Labrus bergylta (Almada et al, 2017)).…”

Section: Estimates Of Contemporary Effective Population Sizementioning

confidence: 99%

Time matters: genetic composition and evaluation of effective population size in temperate coastal fish species

Francisco

Robalo

2020

PeerJ

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Background Extensive knowledge on the genetic characterization of marine organisms has been assembled, mainly concerning the spatial distribution and structuring of populations. Temporal monitoring assesses not only the stability in genetic composition but also its trajectory over time, providing critical information for the accurate forecast of changes in genetic diversity of marine populations, particularly important for both fisheries and endangered species management. We assessed fluctuations in genetic composition among different sampling periods in the western Portuguese shore in three fish species. Methods White seabream Diplodus sargus, sand smelt Atherina presbyter and shanny Lipophrys pholis were chosen, because of their genetic patterns in distinct ecological environments, insight into historical and contemporary factors influencing population effective size (Ne), and degree of commercial exploitation. Samples were obtained near Lisbon between 2003 and 2014 and screened for genetic variation with mitochondrial and nuclear markers. Analyses included genealogies, genetic diversities, temporal structures and contemporary Ne. Results For mtDNA no temporal structure was detected, while for nDNA significant differences were recorded between some sampling periods for the shanny and the sand smelt. Haplotype networks revealed deep genealogies, with various levels of diversification. The shanny revealed a smaller Ne/generation when compared to the other species, which, in turn, revealed no evidence of genetic drift for most study periods. These results highlight the fact that temporal variations in genetic pool composition should be considered when evaluating the population structure of fish species with long distance dispersal, which are more vulnerable to recruitment fluctuations.

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Section: Estimates Of Contemporary Effective Population Sizementioning

confidence: 99%

Time matters: genetic composition and evaluation of effective population size in temperate coastal fish species

Francisco

Robalo

2020

PeerJ

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“…A possible explanation for these results is that during glacial maxima the polar front extended far south and reached the north of the Bay of Biscay, the demographic expansion observed in Porcupine Seabight could be due to recolonization from southern glacial refugia when new areas became habitable (Almada et al, 2017). This is a general trend observed for marine species in the eastern Atlantic Ocean such as teleosts like the rusty blenny (Parablennius sanguinolentus; Pappalardo et al, 2017) or the peacock blenny (Salaria pavo; Castilho et al, 2017); elasmobranchs (Raja clavata; Chevolot et al, 2006) or benthic organisms like seaweed (Chondrus crispus; Provan and Maggs, 2012).…”

Section: Tablementioning

confidence: 83%

“…Due to the shallow nature of the Gibraltar strait and the known vertical distribution of L. lepidion, this connectivity can be mostly explained by the pelagic stages mobility. In fact, differences on both sides of the Gibraltar Strait have been detected in populations of deep-sea elasmobranchs that lack these life stages (Catarino et al, 2015(Catarino et al, , 2017Gubili et al, 2016), and in shallow water fishes (Pappalardo et al, 2017;Fruciano et al, 2011). Actually, the presence of two different Atlantic/Mediterranean partially mixed genetic lineages in L. lepidion seems to suggest a scenario of isolation with secondary contact between the two basins (Castilho et al, 2017).…”

Section: Tablementioning

confidence: 99%

Phylogeography highlights two different Atlantic/Mediterranean lineages and a phenotypic latitudinal gradient for the deep-sea morid codling Lepidion lepidion (Gadiformes: Moridae)

Barros-García¹,

Froufe²,

Bañón

et al. 2020

Deep Sea Research Part I: Oceanographic Research Papers

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“…Several molecular marker and methodologies have been proposed to be applied for authentication of species [ 13 ] and the mitochondrial genes (Cytochrome Oxidase I, cytochrome b, control region, 16SrDNA) are the most used molecular markers [ 14 ]. However, while the barcoding methodology based on sequencing of the mitochondrial Cytochrome Oxidase I gene (COI) has become the marker of choice for identifying animal species and fish species in particular [ 15 , 16 ], the other mitochondrial genes and the control region in particular, have been most used for studies of population genetic structure [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ]. Indeed, after almost 20 years since Hebert et al [ 29 ] proposed the COI as a tool for a global bioidentification system for animals [ 15 , 30 , 31 , 32 ], COI barcode is being worldwide used to unveil commercial seafood fraud based on mislabeling [ 2 , 33 , 34 , 35 , 36 , 37 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products

Pappalardo

Giuga

Raffa

et al. 2022

Foods

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The DNA analysis is the best approach to authenticate species in seafood products and to unveil frauds based on species substitution. In this study, a molecular strategy coupling Cytochrome Oxidase I (COI) DNA barcoding with the consolidated methodology of Restriction Fragment Length Polymorphisms (RFLPs), named COIBar-RFLP, was applied for searching pattern of restriction enzyme digestion, useful to discriminate seven different fish species (juveniles of Engraulis encrasicolus and Sardina pilchardus sold in Italy as “bianchetto” and Aphia minuta sold as “rossetto”; icefish Neosalanx tangkahkeii; European perch, Perca fluviatilis and the Nile Perch, Lates niloticus; striped catfish, Pangasianodon hypophthalmus). A total of 30 fresh and frozen samples were processed for DNA barcoding, analyzed against a barcode library of COI sequences retrieved from GenBank, and validated for COIBar–RFLP analysis. Cases of misdescription were detected: 3 samples labeled as “bianchetto” were substituted by N. tangkahkeii (2 samples) and A. minuta (1 sample); 3 samples labeled as “persico reale” (P. fluviatilis) were substituted by L. niloticus and P. hypophthalmus. All species were simultaneously discriminated through the restriction pattern obtained with MspI enzyme. The results highlighted that the COIBar-RFLP could be an effective tool to authenticate fish in seafood products by responding to the emerging interest in molecular identification technologies.

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Mitochondrial and nuclear intraspecific variation in the rusty blenny (Parablennius sanguinolentus, Blenniidae)

Cited by 6 publications

References 76 publications

Time matters: genetic composition and evaluation of effective population size in temperate coastal fish species

Time matters: genetic composition and evaluation of effective population size in temperate coastal fish species

Phylogeography highlights two different Atlantic/Mediterranean lineages and a phenotypic latitudinal gradient for the deep-sea morid codling Lepidion lepidion (Gadiformes: Moridae)

COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products

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