Mitochondrial and nuclear ribosomal DNA dataset supports that Paramphistomum leydeni (Trematoda: Digenea) is a distinct rumen fluke species

Ma, Jun; He, Jun-Jun; Li, Guohua; Zhou, Dong‐Hui; Liu, Jianzhi; Liu, Yi; Zhu, Xing‐Quan

doi:10.1186/s13071-015-0823-4

Cited by 27 publications

(26 citation statements)

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“…C. daubneyi was the only species identified. It should be noted that no intraspecific differences were detected in either rumen fluke species, which agrees with results obtained previously by other authors [ 39 , 40 ], who sequenced samples of P. leydeni adults, and [ 18 ] who characterised samples of C. daubneyi from different hosts.…”

Section: Discussionsupporting

confidence: 92%

Rumen fluke in Irish sheep: prevalence, risk factors and molecular identification of two paramphistome species

et al. 2016

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BackgroundRumen flukes are trematode parasites found globally; in tropical and sub-tropical climates, infection can result in paramphistomosis, which can have a deleterious impact on livestock. In Europe, rumen fluke is not regarded as a clinically significant parasite, recently however, the prevalence of rumen fluke has sharply increased and several outbreaks of clinical paramphistomosis have been reported. Gaining a better understanding of rumen fluke transmission and identification of risk factors is crucial to improve the control of this parasitic disease. In this regard, a national prevalence study of rumen fluke infection and an investigation of associated risk factors were conducted in Irish sheep flocks between November 2014 and January 2015. In addition, a molecular identification of the rumen fluke species present in Ireland was carried out using an isolation method of individual eggs from faecal material coupled with a PCR. After the DNA extraction of 54 individual eggs, the nuclear fragment ITS-2 was amplified and sequenced using the same primers.ResultsAn apparent herd prevalence of 77.3 % was determined. Several risk factors were identified including type of pasture grazed, regional variation, and sharing of the paddocks with other livestock species. A novel relationship between the Suffolk breed and higher FEC was reported for the first time. The predominant rumen fluke species found was C. daubneyi. Nevertheless, P. leydeni was unexpectedly identified infecting sheep in Ireland for the first time.ConclusionsAn exceptionally high prevalence of rumen fluke among Irish sheep flocks has been highlighted in this study and a more thorough investigation is necessary to analyse its economic impact. The isolation of individual eggs coupled with the PCR technique used here has proven a reliable tool for discrimination of Paramphistomum spp. This technique may facilitate forthcoming studies of the effects of paramphistomosis on livestock production. The most noteworthy finding was the identification of P. leydeni affecting sheep in Ireland, however further studies are required to clarify its implications. Also, a significant relationship between Suffolk breed and a heavier infection was found, which can be used as a starting point for future research on control strategies of rumen fluke infection.

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Section: Discussionsupporting

confidence: 92%

Rumen fluke in Irish sheep: prevalence, risk factors and molecular identification of two paramphistome species

et al. 2016

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“…Besides, the nad 5 is highly variable, and genes of nad 4L and cyt b are rather conserved. These characteristics are in accordance with flukes of the families Paramphistomatidae and Notocotylidae [ 18 , 28 ].…”

Section: Resultssupporting

confidence: 74%

Complete mitochondrial genome of the giant liver fluke Fascioloides magna (Digenea: Fasciolidae) and its comparison with selected trematodes

et al. 2016

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BackgroundRepresentatives of the trematode family Fasciolidae are responsible for major socio-economic losses worldwide. Fascioloides magna is an important pathogenic liver fluke of wild and domestic ungulates. To date, only a limited number of studies concerning the molecular biology of F. magna exist. Therefore, the objective of the present study was to determine the complete mitochondrial (mt) genome sequence of F. magna, and assess the phylogenetic relationships of this fluke with other trematodes based on the mtDNA dataset.FindingsThe complete F. magna mt genome sequence is 14,047 bp. The gene content and arrangement of the F. magna mt genome is similar to those of Fasciola spp., except that trnE is located between trnG and the only non-coding region in F. magna mt genome. Phylogenetic relationships of F. magna with selected trematodes using Bayesian inference (BI) was reconstructed based on the concatenated amino acid sequences for 12 protein-coding genes, which confirmed that the genus Fascioloides is closely related to the genus Fasciola; the intergeneric differences of amino acid composition between the genera Fascioloides and Fasciola ranged 17.97–18.24 %.ConclusionsThe determination of F. magna mt genome sequence provides a valuable resource for further investigations of the phylogeny of the family Fasciolidae and other trematodes, and represents a useful platform for designing appropriate molecular markers.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1699-7) contains supplementary material, which is available to authorized users.

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“…gigantica and Fa. hepatica (12.2%) [ 20 ], between Paramphistomum cervi and P. leydeni (14.7%) [ 23 ], and between Paragonimus westermani isolates from Korea and India (20.74%) [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

“…The accurate identification of trematode species is relevant in relation to better understand their biology, epidemiology and ecology, and has implications for the diagnosis of infections. Genetic markers in mtDNA and rDNA have been proven effective for identifying trematode species [ 23 , 24 ] that cannot be differentiated by morphological analysis, including flukes in the family Fasciolidae [ 10 ]. Here, we determined the sequences of the complete mt genome and ITS-1, ITS-2 and partial 18S rDNA sequences of F. buski from China, and compared these with sequences derived from isolates from India and Vietnam to test the hypothesis that the isolate of F. buski sampled in China may represent a genetically distinct taxon from that sampled in India.…”

Section: Introductionmentioning

confidence: 99%

Fasciolopsis buski (Digenea: Fasciolidae) from China and India may represent distinct taxa based on mitochondrial and nuclear ribosomal DNA sequences

Ma¹,

Sun

et al. 2017

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Background Fasciolopsis buski is a zoonotic intestinal fluke infecting humans and pigs, but it has been seriously neglected. It is yet to know whether there is any genetic diversity among F. buski from different geographical locations, particularly in sequences of nuclear ribosomal DNA (rDNA) and mitochondrial (mt) DNA. Therefore, we determined the sequences of partial 18S, the complete internal transcribed spacer (ITS) rDNA and the complete mt genome of F. buski from China, compared the rDNA and mtDNA sequences with those of isolates from India and Vietnam, and assessed the phylogenetic relationships of this fluke and related fasciolid trematodes based on the mtDNA dataset.ResultsThe complete mt genome sequence of F. buski from China is 14,833 bp, with 36 genes, including 12 protein-coding genes (PCGs), 22 tRNA genes, and two rRNA genes (rrnL and rrnS). The AT content of F. buski from China is 65.12%. The gene content and arrangement of the F. buski mt genome is similar to that of Fascioloides magna. Genetic distances between isolates of F. buski from China and India were high (28.2% in mtDNA, 13.2% in ITS-1 and 9.8% in ITS-2) and distinctly higher than the interspecific differences between Fasciola hepatica and Fasciola gigantica. The rDNA and mtDNA datasets for F. buski from China (isolate from pigs) and Vietnam (isolates from humans) were identical. The intergeneric differences in amino acid and nucleotide sequences among the genera Fasciolopsis, Fascioloides and Fasciola ranged between 24.64–25.56% and 26.35–28.46%, respectively.ConclusionsOur results indicate that F. buski from China and India may represent distinct taxa, while F. buski in Vietnam and China represent the same species. These findings might have implications for the implementation of appropriate control strategies in different regions. Further studies are needed to decode mtDNA and rDNA sequences of F. buski from various geographical isolates for the better understanding of the species complex of F. buski.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-017-2039-2) contains supplementary material, which is available to authorized users.

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Mitochondrial and nuclear ribosomal DNA dataset supports that Paramphistomum leydeni (Trematoda: Digenea) is a distinct rumen fluke species

Cited by 27 publications

References 41 publications

Rumen fluke in Irish sheep: prevalence, risk factors and molecular identification of two paramphistome species

Rumen fluke in Irish sheep: prevalence, risk factors and molecular identification of two paramphistome species

Complete mitochondrial genome of the giant liver fluke Fascioloides magna (Digenea: Fasciolidae) and its comparison with selected trematodes

Fasciolopsis buski (Digenea: Fasciolidae) from China and India may represent distinct taxa based on mitochondrial and nuclear ribosomal DNA sequences

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