Mitochondrial aspartate aminotransferase in myocardial infarction

Murros, Juhani; Konttinen, Aarne; Somer, Hannu

doi:10.1016/0009-8981(73)90373-2

Cited by 18 publications

(3 citation statements)

References 7 publications

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“…In contrast to this and other previous reports (Fleisher and Wakim, 1961;Bodansky et al, 1966;Murros et al, 1973), all the patients with acute myocardial infarction in our study had abnormally high m-AST activities. This difference suggests that our immunological method, using the sensitised erythrocytes of sheep to eliminate the antigen-antibody complex of s-AST, has higher sensitivity than the previous technique.…”

Section: (4) Serum M-ast Activity In Patients With Liver Diseasecontrasting

confidence: 99%

“…These results agree with the report by Murros et al (1973) that serum m-AST activity determined by electrophoresis increased to above normal in 23 of 24 patients with acute myocardial infarction and that its peak level was usually obtained later than that of t-AST, indicating the delayed release of m-AST into the circulation.…”

Section: (4) Serum M-ast Activity In Patients With Liver Diseasesupporting

confidence: 93%

“…This difference suggests that our immunological method, using the sensitised erythrocytes of sheep to eliminate the antigen-antibody complex of s-AST, has higher sensitivity than the previous technique. Indeed we detected serum m-AST activity in 31 of 35 patients with liver disease, though Murros et al (1973) could not detect m-AST activity in the serum of their patients with liver disease whose serum t-AST was less than 100 KU/ml, even with the immunological technique.…”

Section: (4) Serum M-ast Activity In Patients With Liver Diseasementioning

confidence: 61%

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Immunological determination of serum m-AST activity in patients with acute myocardial infarction.

Inoue¹,

Hori²,

Nishimoto³

et al. 1978

Heart

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Serum m-AST (mitochondrial isoenzyme of AST) activity in patients with acute myocardial infarction was determined quantitatively by a new immunological technique which is sensitive and easily available. All 31 patients with acute myocardial infarction showed abnormally high levels of serum m-AST (more than 5 KU/ml); the mean serum m-AST activity attained its peak (42.0 +/- 4.9 KU/ml) on the first day after the onset of infarction 5 hours later than that of serum t-AST (total AST) activity in 15 patients whose peak m- and t-AST activities were identified clearly. The individual peak m-AST activity correlated with the total CK released (r = 0.83, n = 15), indicating that the release of m-AST also reflects the infarct size. The ratio of serum m-AST/t-AST increased following myocardial infarction and showed the maximal value (average 25.7%) on the third to seventh day after onset. This ratio in the patients with acute myocardial ifarction was also greater than that in patients with liver disease or with heart failure from causes other than acute myocardial infarction. In the patients who had the additional complication of heart failure and/or cardiogenic shock the ratio was also greater than that is the patients without these hazards. These results indicate that the ratio of serum m-AST/t-AST reflects the severity of the myocardial cellular damage in acute myocardial infarction.

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Section: (4) Serum M-ast Activity In Patients With Liver Diseasecontrasting

confidence: 99%

Section: (4) Serum M-ast Activity In Patients With Liver Diseasesupporting

confidence: 93%

Section: (4) Serum M-ast Activity In Patients With Liver Diseasementioning

confidence: 61%

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Immunological determination of serum m-AST activity in patients with acute myocardial infarction.

Inoue¹,

Hori²,

Nishimoto³

et al. 1978

Heart

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Optimal conditions for protease use in the assay of serum mitochondrial aspartate aminotransferase

Okabe

Uji

Sugiuchi

et al. 1990

Clinical Laboratory Analysis

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The optimal conditions for selective proteolytic inactivation of cytosolic aspartate aminotransferase (c-AST) to determine mitochondrial aspartate aminotransferase (m-AST) in serum were studied. Protease 401 was found to be effective over a pH range of 6.0-10.0. A pH of 9.5 with 0.5% albumin in the reagent mixture was determined to be optimal for inactivation of c-AST and preservation of m-AST, lactic dehydrogenase (LDH), and malic dehydrogenase (MDH) in the assay procedure. The presence of serum endogenous protein inhibitors such as alpha 1-antitrypsin and alpha 2-macroglobin did not inhibit protease 401.

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The SZASZ-ratio (CK/GOT) as example for the diagnostic significance of enzyme ratios in serum

Schmidt

Chemnitz

et al. 1980

Klin Wochenschr

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The diagnostic significance of enzyme and isoenzyme ratios in serum relies on their different distribution at various levels: from organs to subcellular structures. Factors which modify the ratios are the molecular properties of enzymes and isoenzymes and their turnover in the cells and in the extracellular space. These pathophysiological principles are considered with respect to the Szasz ratio CK/GOT and its application for the differential diagnosis of acute myocardial infarction.

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Mitochondrial aspartate aminotransferase in myocardial infarction

Cited by 18 publications

References 7 publications

Immunological determination of serum m-AST activity in patients with acute myocardial infarction.

Immunological determination of serum m-AST activity in patients with acute myocardial infarction.

Optimal conditions for protease use in the assay of serum mitochondrial aspartate aminotransferase

The SZASZ-ratio (CK/GOT) as example for the diagnostic significance of enzyme ratios in serum

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