Mitochondrial atpA gene is altered in a new orf220-type cytoplasmic male-sterile line of stem mustard (Brassica juncea)

Yang, Jinghua; Huai, Yan; Zhang, Mingfang

doi:10.1007/s11033-007-9176-1

Cited by 39 publications

(24 citation statements)

References 34 publications

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“…Westar (Sakai and Imamura 1992), Beta vulgaris (Xue et al 1994) and Brassica juncea (Yang et al 2009). In all these reports, the expression of atpA gene was all affected due to the genomic rearrangements in the atpA locus.…”

Section: Pcr Amplification With Cms-specific Scar Primer Pairsmentioning

confidence: 99%

Molecular characterization of cytoplasmic male sterility conditioned by Gossypium harknessii cytoplasm (CMS-D2) in upland cotton

Gong

Cui

et al. 2011

Euphytica

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Cytoplasmic male sterility (CMS) is a maternally inherited trait that fails to produce functional pollen grains. The CMS system is widely employed to facilitate the utilization of heterosis in major crops. However, little is known about the CMS associated genes in Upland cotton (Gossypium hirsutum). The objective of this study was to compare CMS cotton (CMS-D2) with the cytoplasm from G. harknessii and its isogenic maintainer line with the normal fertile Upland cotton cytoplasm to identify CMS-D2 specific gene(s) and to develop CMSspecific sequence characterized amplified region (SCAR) markers. Based on Southern blot analysis using 10 mitochondrial gene-specific probes (cob, cox2, atp6, atp9, nad3, cox3, atpA, cox1, nad6 and nad9), three probes (cox3, atpA, and nad6) revealed restriction fragment length polymorphisms (RFLP) between the CMS-D2 and its isogenic maintainer line. RT-PCR confirmed that the three genes were differentially expressed between the two lines. These results indicated that there existed structural and expression variations in the three genes when the mitochondrial D2 genome was transferred into Upland cotton. Genome walking and rapid amplification of cDNA ends (RACE) were further performed to analyze the sequences of these genes and their flanking regions. For cox3 and nad6, there was only one different nucleotide each in the gene regions between the two lines. Also some nucleotides upstream of the ATG codon were different. For atpA, the sequences downstream the atpA were significantly different between the two cytoplasmic lines. Furthermore, two nucleotides at the -4 and -5 position from ATG codon were also changed between the two cytoplasms (i.e., CG?AA), and this mutation also exists in RNA sequences. Interestingly, nine nucleotides (ATGCAACTA) were also inserted at the location of 899 bp upstream of ATG codon in the CMS line. The results suggest that the abnormal sequence and expression of atpA gene is associated with CMS expression in Upland cotton. According to the significant different sequences downstream the atpA gene, a CMS-D2 specific SCAR marker was developed. The CMS-specific PCR bands were verified for 10 cultivars containing either normal-or CMS-D2cytoplasm. This will allow quick and reliable Jianyong Wu and Yangcang Gong contributed equally to this work.Electronic supplementary material The online version of this article (

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Section: Pcr Amplification With Cms-specific Scar Primer Pairsmentioning

confidence: 99%

Molecular characterization of cytoplasmic male sterility conditioned by Gossypium harknessii cytoplasm (CMS-D2) in upland cotton

Gong

Cui

et al. 2011

Euphytica

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“…tumida Tsen et Lee) and its maintainer line were as described by colleagues (Chen et al 1995;Yang et al 2008a). The CMS stem mustard line was synthesized by distant hybridizations and subsequent back-crossings between Brassica campestris and Brassica juncea.…”

Section: Plant Materialsmentioning

confidence: 99%

“…1). In CMS stem mustard, we previously reported that CMS plants developed homeotic floral organs, in which stamens were replaced by other floral organ structures, such as, petaloidy, pinnate, carpelloidy or silk-like floral structure (Yang et al 2008a).…”

Section: Introductionmentioning

confidence: 99%

MADS-box genes are associated with cytoplasmic homeosis in cytoplasmic male-sterile stem mustard as partially mimicked by specifically inhibiting mtETC

Yang

Zhang

et al. 2008

Plant Growth Regul

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In present investigation, we studied how mitochondrial-nuclear interactions may give rise to the third abnormal whorl floral organ in cytoplasmic male sterility (CMS) stem mustard, which exhibited complete conversion of homeotic transformations of stamens into other floral organ structures. In this system, expressions of AP3-like, PI-like and AG-like genes, the counterpart of the Arabidopsis nuclear ABC model genes related to floral organ development, were specifically altered in floral organs of CMS plants. When mitochondrial-specific inhibitor was applied to wild type fertile stem mustard plants, expressions of AP3-like, PI-like and AG-like genes were repressed. As a consequence, abnormal floral organs were observed in its maintainer line treated with mitochondrial-specific inhibitor. Thereby, we suggest that nuclear MADS-box transcription factors subject to mitochondrial retrograde regulation were associated with cytoplasmic homeosis in CMS stem mustard and could be mimicked by specifically inhibiting mtETC.

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“…Novel and chimeric mitochondrial sequences are a frequent result of this recombination (Wise et al, 1987;Kennell and Pring, 1989;Wen and Chase, 1999;Gallagher et al, 2002;Okazaki et al, 2013;Yamagishi and Bhat, 2014;Tang et al, 2017), in which recombination sometimes leads to the creation of transcripts that interfere with normal male gametophyte development (Kitazaki and Kubo, 2010) via the generation of toxic and/or disruptive transmembrane proteins (Korth et al, 1991;Kim et al, 2007;Wan et al, 2007;Zhang et al, 2007;Yang et al, 2009;Gulyas et al, 2010;Jing et al, 2012;Flores-Renteria et al, 2013;Ji et al, 2013;Luo et al, 2013;Okazaki et al, 2013;Park et al, 2013;Hu et al, 2014). Surprisingly, such genes are not only abundant in many fertile plants, such as Arabidopsis thaliana Unseld et al, 1997), Beta vulgaris (Kubo et al, 2000), Oryza sativa (Notsu et al, 2002), Brassica napus (Handa, 2003), Zea mays (Clifton et al, 2004), Triticum aestivum (Ogihara et al, 2005), and Nicotiana tabacum (Sugiyama et al, 2005), but are also constitutively expressed.…”

Section: Discussionmentioning

confidence: 99%

Plant Mitochondrial Genome Evolution and Cytoplasmic Male Sterility

Chen

Zhao

et al. 2017

Critical Reviews in Plant Sciences

128

116

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Mitochondrial atpA gene is altered in a new orf220-type cytoplasmic male-sterile line of stem mustard (Brassica juncea)

Cited by 39 publications

References 34 publications

Molecular characterization of cytoplasmic male sterility conditioned by Gossypium harknessii cytoplasm (CMS-D2) in upland cotton

Molecular characterization of cytoplasmic male sterility conditioned by Gossypium harknessii cytoplasm (CMS-D2) in upland cotton

MADS-box genes are associated with cytoplasmic homeosis in cytoplasmic male-sterile stem mustard as partially mimicked by specifically inhibiting mtETC

Plant Mitochondrial Genome Evolution and Cytoplasmic Male Sterility

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