Mitochondrial bioenergetics in leukocytes and oxidative stress in blood serum of mild to moderately depressed women

Gumpp, Anja M.; Behnke, Alexander; Bach, Alexandra M.; Piller, Sophia; Boeck, Christina; Rojas, Roberto; Kolassa, Iris‐Tatjana

doi:10.1016/j.mito.2020.12.009

Cited by 9 publications

(3 citation statements)

References 71 publications

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“…To generalize our findings and investigate whether CM-related alterations in mitochondrial bioenergetics become evident specifically under allostatic load, additional research is needed among men and women with concurrent physical or psychosocial stress. The mitochondrial bioenergetic profile was measured in cryopreserved cells and cryopreservation per se has an impact on the quantitative measures related to oxygen consumption rates (Gumpp et al, 2021 ). Although storage time of cryopreserved cells was statistically considered, we can thus not exclude that freezing procedure and cryopreservation might have differentially affected the immune cells of individuals with and without CM.…”

Section: Discussionmentioning

confidence: 99%

“…Recent research linked alterations in mitochondrial bioenergetics to various adverse mental and physical health conditions (Daniels, Olsen, & Tyrka, 2020 ; Srivastava, 2017 ). For example, changes in mitochondrial bioenergetics were observed in major depression as a highly prevalent mental health consequence of CM (Gumpp et al, 2021 ; Hroudová, Fišar, Kitzlerová, Zvěřová, & Raboch, 2013 ; Karabatsiakis et al, 2014 ; Kuffner et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Investigating mitochondrial bioenergetics in peripheral blood mononuclear cells of women with childhood maltreatment from post-parturition period to one-year follow-up

et al. 2022

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Background Childhood maltreatment (CM) exerts various long-lasting psychological and biological changes in affected individuals, with inflammation being an interconnecting element. Besides chronic low-grade inflammation, CM might also affect the energy production of cells by altering the function and density of mitochondria, i.e. the body's main energy suppliers. Here, we compared mitochondrial respiration and density in intact peripheral blood mononuclear cells (PBMC), from women with and without CM between two time points, i.e. at the highly inflammatory phase within 1 week after parturition (t0) and again after 1 year (t2). Methods CM exposure was assessed with the Childhood Trauma Questionnaire. Whole blood was collected from n = 52 healthy women within the study ‘My Childhood – Your Childhood’ at both time points to isolate and cryopreserve PBMC. Thawed PBMC were used to measure mitochondrial respiration and density by high-resolution respirometry followed by spectrophotometric analyses of citrate-synthase activity. Results Over time, quantitative respiratory parameters increased, while qualitative flux control ratios decreased, independently of CM. Women with CM showed higher mitochondrial respiration and density at t0, but not at t2. We found significant CM group × time interaction effects for ATP-turnover-related respiration and mitochondrial density. Conclusions This is the first study to longitudinally investigate mitochondrial bioenergetics in postpartum women with and without CM. Our results indicate that CM-related mitochondrial alterations reflect allostatic load, probably due to higher inflammatory states during parturition, which normalize later. However, later inflammatory states might moderate the vulnerability for a second-hit on the level of mitochondrial bioenergetics, at least in immune cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Investigating mitochondrial bioenergetics in peripheral blood mononuclear cells of women with childhood maltreatment from post-parturition period to one-year follow-up

et al. 2022

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“…The major depressive disorder (MMD) is one of the most common mood disorders characterized by sadness, depres-sion, and irritability, which affect the physical and cognitive functions of patients [1][2][3][4][5]. Although the etiology of MDD is not fully clear, there is increasing evidence suggesting the involvement of a specific component of metabolic stress and oxidative stress, in the pathophysiology of depression [6]. Oxidative stress refers to the biologically damaging effects of free radicals or reactive oxygen [7], which in excess cause damage to lipids, proteins, and DNA and can ultimately result in cell death.…”

Section: Introductionmentioning

confidence: 99%

Association of the Combined Effects between Insulin-Like Growth Factor-1 Gene Polymorphisms and Negative Life Events with Major Depressive Disorder among Chinese population in the Context of Oxidative Stress

Qiao

Xie

Yong-mei

et al. 2022

Oxidative Medicine and Cellular Longevity

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Background. Oxidative stress may be increased in a number of psychiatric disorders, including major depressive disorder (MDD). MDD has been shown to be related to insulin-like growth factor-1 (IGF-1) as well as to negative life events; exploring the interaction of IGF-1 polymorphisms and negative life events on the risk of MDD is needed. The aim of this study was to analyze the single and combined effects of IGF-1 polymorphisms (rs972936 and rs978458) and negative life events with MDD among Chinese population. Methods. 420 MDD patients (according to DSM-V) and 420 age- and gender-matched control subjects were recruited in a case-control study. Negative life events were assessed using standard rating scales. IGF-1 rs972936 and rs978458 were identified by sequencing. The chi-square ( χ 2 ) tests were performed to explore the association of negative life events and IGF-1 polymorphisms with MDD. Results. Our results found that the negative life events were associated with the risk of MDD ( P < 0.001 ; OR = 3.28 , 95% CI: 2.19-4.85). The genotypes of IGF-1 were associated with the risk of MDD ( P < 0.001 ); carrying the IGF-1 rs972936 C allele ( OR = 1.53 , 95% CI: 1.26-1.85) and rs978458 T allele ( OR = 1.92 , 95% CI: 1.58-2.34) had a higher risk of MDD. The combined effects between IGF-1 rs978458 and negative life events were associated with the risk of MDD ( P < 0.05 ; OR = 2.94 , 95% CI: 1.23-7.03), but IGF-1 rs972936 was not associated ( P > 0.05 ). Conclusions. Based on the oxidative stress hypothesis, we confirm that carrying IGF-1 rs972936 C allele and rs978458 T allele have a higher risk of MDD and the combined effects between IGF-1 rs978458 and negative life events were associated with the risk of MDD among Chinese population.

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Age-related changes in energy metabolism in peripheral mononuclear blood cells (PBMCs) and the brains of cognitively healthy seniors

Silaidos¹,

Reutzel²,

Wachter³

et al. 2023

GeroScience

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Mitochondrial dysfunction is a hallmark of cellular senescence and many age-related neurodegenerative diseases. We therefore investigated the relationship between mitochondrial function in peripheral blood cells and cerebral energy metabolites in young and older sex-matched, physically and mentally healthy volunteers. Cross-sectional observational study involving 65 young (26.0 ± 0.49 years) and 65 older (71.7 ± 0.71 years) women and men recruited. Cognitive health was evaluated using established psychometric methods (MMSE, CERAD). Blood samples were collected and analyzed, and fresh peripheral blood mononuclear cells (PBMCs) were isolated. Mitochondrial respiratory complex activity was measured using a Clarke electrode. Adenosine triphosphate (ATP) and citrate synthase activity (CS) were determined by bioluminescence and photometrically. N-aspartyl-aspartate (tNAA), ATP, creatine (Cr), and phosphocreatine (PCr) were quantified in brains using 1H- and 31P-magnetic resonance spectroscopic imaging (MRSI). Levels of insulin-like growth factor 1 (IGF-1) were determined using a radio-immune assay (RIA). Complex IV activity (CIV) (− 15%) and ATP levels (− 11%) were reduced in PBMCs isolated from older participants. Serum levels of IGF-1 were significantly reduced (− 34%) in older participants. Genes involved in mitochondrial activity, antioxidant mechanisms, and autophagy were unaffected by age. tNAA levels were reduced (− 5%), Cr (+ 11%), and PCr (+ 14%) levels were increased, and ATP levels were unchanged in the brains of older participants. Markers of energy metabolism in blood cells did not significantly correlate with energy metabolites in the brain. Age-related bioenergetic changes were detected in peripheral blood cells and the brains of healthy older people. However, mitochondrial function in peripheral blood cells does not reflect energy related metabolites in the brain. While ATP levels in PBMCs may be be a valid marker for age-related mitochondrial dysfunction in humans, cerebral ATP remained constant.

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Mitochondrial bioenergetics in leukocytes and oxidative stress in blood serum of mild to moderately depressed women

Cited by 9 publications

References 71 publications

Investigating mitochondrial bioenergetics in peripheral blood mononuclear cells of women with childhood maltreatment from post-parturition period to one-year follow-up

Investigating mitochondrial bioenergetics in peripheral blood mononuclear cells of women with childhood maltreatment from post-parturition period to one-year follow-up

Association of the Combined Effects between Insulin-Like Growth Factor-1 Gene Polymorphisms and Negative Life Events with Major Depressive Disorder among Chinese population in the Context of Oxidative Stress

Age-related changes in energy metabolism in peripheral mononuclear blood cells (PBMCs) and the brains of cognitively healthy seniors

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