Mitochondrial Ca2+ uptake is essential for synaptic plasticity in pain

Mitochondrial CaMKII inhibition in airway epithelium protects against allergic asthma

“…Activation of NLRP3 in dendritic cells by bacterial challenge identified a role for NLRP3/caspase-1/IL-18 in promoting allergic asthma (78). In addition, in diet-induced obesity, NLRP3 induces innate lymphoid cell-mediated AHR (79). Relevant to our study, the NLRP3 pathway has also been implicated in OVA-induced asthma (51), and we extend these findings by demonstrating NLRP3 activation in the A. fumigatus model.…”

Mitochondrial dynamics in neuronal injury, development and plasticity

“…5), which is thought to underlie the development of chronic pain. In mice, depolarizing mitochondria or blocking the MCU interfered with N-methyl-Daspartate (NMDA)-induced Ca 2+ rises in mitochondria but not in the cytosol, therefore attenuating spinal LTP and mechanical hyperalgesia following injury (Kim et al, 2011).…”

Role of mitochondrial Ca2+ uniporter in remifentanil‐induced postoperative allodynia

“…Ru360 (Calbiochem, Billerica, MA, USA) was dissolved in saline at 20 or 50 lM. Rats were injected with 10 lL of Ru360 (20 or 50 lM) or vehicle (saline) intrathecally at the L5/L6 intervertebral space 30 min before incision, as described by Kim et al (2011).…”

“…Rhod-2/AM is cationic, and after loading into the cells, its uptake into the mitochondrial is driven by the potential across the mitochondrial membrane. In this study, loading of Rhod-2/AM in rat spinal tissues for mitochondrial Ca 2+ imaging was performed according to a protocol established by Kim et al (2011), which has been optimized selective Rhod-2 loading in the mitochondria. In brief, 10 lL of 33 lM Rhod-2/AM stock solution (Invitrogen) with or without Ru360 (10 lL of stock solution at 50 lM) was injected intrathecally in rats 30 min before remifentanil infusion.…”

“…In brief, 10 lL of 33 lM Rhod-2/AM stock solution (Invitrogen) with or without Ru360 (10 lL of stock solution at 50 lM) was injected intrathecally in rats 30 min before remifentanil infusion. One hour after the completion of remifentanil infusion, rats were perfused with saline followed by 4% EDC (Kim et al, 2011). Spinal cords at L4/5 were collected, fixed, and cryoprotected in 30% sucrose.…”