Mitochondrial characteristics in oocytes of the domestic cat (<i>Felis catus</i>) after in vitro maturation and vitrification

Sowińska, Natalia; Müller, Karin; Niżański, Wojciech; Jewgenow, Katarina

doi:10.1111/rda.12982

Cited by 10 publications

(7 citation statements)

References 46 publications

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“…Previous studies have recently shown that mitochondria in human oocytes at the GV stage have a heterogeneous distribution but after GVBD this configuration changed rapidly to a homogeneous distribution (Takahashi et al ., 2016). In contrast, cat oocytes either in GV or MII predominantly have mitochondria distributed in the peripheral region (Sowińska et al ., 2017). In sheep, migration of mitochondria to the periphery of the oocyte was observed after 6 h of IVM in COCs from follicles with a diameter of approximately 2 mm (Máximo et al ., 2012).…”

Section: Discussionmentioning

confidence: 99%

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Influence of interleukin 1 beta and tumour necrosis factor alpha on the in vitro growth, maturation and mitochondrial distribution of bovine oocytes from small antral follicles

Lima

Bezerra

Souza

et al. 2018

Zygote

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SummaryThis study aimed to investigate the effects of IL1β and TNFα on growth and maturation of oocytes from small follicles (1–3 mm) during in vitro culture. To this end, cumulus–oocyte complexes (COCs) with diameters of ~110 µm were cultured in TCM-199 medium alone or supplemented with IL1β (10 ng/ml), TNFα (10 ng/ml) or both for 48 h. The oocytes were measured at the beginning and at the end of the culture period. COCs were cultured for 20 h in pre-maturation medium and then half of the COCs of each group was destined for in vitro maturation and the remaining COCs were used to evaluate meiotic progression, mitochondrial distribution and the expression of mRNAs for GDF-9, c-Mos, Cyclin-B1 and H1foo. The results showed that COCs cultured with TNFα alone or together with IL1β had higher diameters than those cultured in control medium alone or supplemented with IL1β. Control oocytes isolated from large antral follicles (>5 mm) had heterogeneous distribution of mitochondria. Oocytes isolated from small antral follicles, that had been grown in vitro in TCM-199 alone or supplemented with TNFα had similar heterogeneous mitochondrial distribution before in vitro maturation (IVM). After IVM, mitochondria were heterogeneously distribution when cultured in TCM-199. However, when cultured with TNFα and/or IL1β, mitochondria were homogeneously distributed. Presence of TNFα and/or IL1β in TCM-199 culture medium did not influence the expression of mRNAs for GDF-9, c-Mos, Cyclin-B1 and H1foo. In conclusion, TNFα and a mixture of TNFα and IL1β both stimulated the growth of bovine oocytes during their in vitro culture, but do not influence gene expression in grown oocytes.

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Section: Discussionmentioning

confidence: 99%

“…predominantly have mitochondria distributed in the peripheral region (Sowińska et al, 2017). In sheep, migration of mitochondria to the periphery of the oocyte was observed after 6 h of IVM in COCs from follicles with a diameter of approximately 2 mm (Máximo et al, 2012).…”

Section: Effects Of Il1β and Tnfα On Oocyte Maturationmentioning

confidence: 99%

Influence of interleukin 1 beta and tumour necrosis factor alpha on the in vitro growth, maturation and mitochondrial distribution of bovine oocytes from small antral follicles

Lima

Bezerra

Souza

et al. 2018

Zygote

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“…In several species and cell types, including gametes, cryopreservation-induced apoptosis has been reported (5)(6)(7)(8)(9)(10)(11)(12)(13)(14). In domestic cat immature oocytes, vitrification affects mitochondria aggregation (15), and probably also the permeability of gap junctions hemichannels, causing the loss of small essential metabolites, ionic imbalance, and the penetration of small, potentially toxic, molecules (16). However, information is still limited, and only one study tried to target the apoptosis pathways through an inhibitor of a signaling molecule known as ROCK (Rho-associated coiled-coil containing protein kinase), obtaining an improvement in the developmental competence of VOs in terms of normal fertilization and cleavage rates (4).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Apoptotic Pathways Improves DNA Integrity but Not Developmental Competence of Domestic Cat Immature Vitrified Oocytes

et al. 2020

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Being a model for endangered wild felids, cryopreservation protocols for domestic cat oocytes are under continuous development. Immature vitrified oocytes (VOs) are a valuable resource for fertility preservation programs, but they often degenerate after warming and their in vitro development is poor. Since the exact mechanisms are not clear, this study assessed whether vitrification might trigger two apoptotic markers (DNA fragmentation and caspase activity, Experiment I) and the effects of a chemical inhibitor (i.e., the pan-caspase inhibitor Z-VAD-FMK) on the same markers (Experiment II) and on VOs in vitro development (Experiment III). The overarching aim was to check whether apoptosis inhibition might be a strategy to improve cat oocytes cryotolerance. In Experiment I, vitrification induced DNA fragmentation and increased caspase activity in VOs incubated for 24 h after warming (DNA fragmentation: 59.38%; caspase activity: 414.6 ± 326.8) compared to a fresh control (9.68%; 199.6 ± 178.3; p = 0.02). In Experiment II, the addition of Z-VAD-FMK to vitrification-warming and incubation media decreased DNA fragmentation and caspase activity (8.82%; 243.7 ± 106.9) compared to control (untreated) VOs (69.44%; 434.5 ± 248.3; p < 0.001). In Experiment III, Z-VAD-FMK brought maturation rates of treated VOs close to those of fresh oocytes (53.13 and 65.38%, respectively, p = 0.057), but there were no differences in VOs embryo development (cleavage rates; Z-VAD-FMK-treated VOs: 34.38%; control VOs: 31.78%; p = 0.69). In summary, vitrification increased apoptotic markers in cat VOs, and while Z-VAD-FMK was able to hinder DNA damage and caspase activity, its addition was not determinant for embryo development. To make the best use of VOs, other oocyte in vitro maturation and embryo culture strategies, such as the addition of other inhibitors or their prolonged use, should be investigated.

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“…It has been reported for both GV and MII stage domestic cat oocytes that vitrification does not disturb the mitochondrial distribution, but causes mitochondrial aggregation and a decreased fluorescence intensity when evaluated using the MitoTracker Red CMXRos staining (199).…”

Section: Discussionmentioning

confidence: 99%

“…There have been several studies which investigated the cryopreserved MII oocyte subcellular structures including cortical granules in humans (191)(192)(193)(194)(195), meiotic spindles in mice (194,196,197), mitochondria distribution in humans and domestic cats (195,198,199) and F-actin in humans (108). Our study simultaneously characterized the vitrified-warmed MII mouse oocyte subcellular structures including meiotic spindles, Factin, CGs and mitochondria.…”

Section: Discussionmentioning

confidence: 99%

Investigation of methods and factors influencing mouse oocyte quality, in vitro fertilization and embryo development

Wuri¹

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Mice are one of the most commonly used rodent species as a model for biomedical research to better understand molecular, genetic, and cellular causes of human disease and disorders. The production of good quality oocytes is one of the important determinant factors for successful assisted reproductive technologies (ARTs) clinical outcome as well as reproductive biology research. Mouse oocyte quality, morphology and functions are influenced by a variety of the factors such as euthanasia methods of female donors, superovulation regimes and cryopreservation. The objectives of these studies were mainly to investigate the methods and factors that are influencing oocyte quality, in-vitro fertilization (IVF) and embryonic development. First, how different euthanasia methods including cervical dislocation (CD), high flow rate CO[2] (H CO[2]) and low flow CO2 (L CO[2]) would affect the quality and integrity of the metaphase II (MII) oocytes have been investigated. Cumulus oocyte complexes (COCs) were collected from female donors that were euthanized by three different methods and then the oocytes' subcellular structures including microtubules, F-actin, cortical granules (CGs) and mitochondria integrities were detected by specific fluorescence dyes. The results showed that L CO[2] caused significant increase in the incidence of premature cortical granule exocytosis (PCGE) which might be responsible for significantly reducing the in-vitro fertilization (IVF) and embryonic development rate compared to CD and H CO[2]. Secondly, how the superovulation methods would affect the resulting oocyte morphology, quality, IVF competence and embryonic development was investigated. The anti-inhibin serum (AIS) superovulation method produced a significantly higher number of oocytes compared to the pregnant mare serum gonadotrophin (PMSG). Overall, both methods yielded oocytes with similar sizes and comparable subcellular structures including microtubules, F-actin, cortical granules and mitochondria. However, superovulation with AIS produced significantly thinner zona pellucide than PMSG and the perivitelline space of the oocytes generated from AIS were significantly larger than PMSG. There were no differences in terms of two-cell embryo development, or morula and blastocyst formation rates between AIS and PMSG when the oocytes from two methods were in-vitro fertilized with fresh sperm. Morula and blastocyst development rates were significantly higher for AIS compared to PMSG when oocytes were fertilized with frozen-thawed sperm. Thirdly, clutches of mouse cumulus oocytes complexes (COCs) were cryopreserved by the cryoloop vitrification method after PMSG superovulation. The cryo-survival rate and integrity and distribution of subcellular structures including the meiotic spindles, F-actin, cortical granules and mitochondria were examined and compared with fresh MII oocytes. The vitrified-warmed oocytes maintained their subcellular structures to a high degree and resulted in acceptable IVF and embryonic development. In conclusion, for optimal research and clinical outcome, considerations should be given regard to euthanasia methods of oocyte donor mice and type of superovulation regimes. Despite of its high oocyte yield, superovulation of mice with AIS provides comparable quality oocytes to the PMSG method. Cryopreservation of the clutches of mouse COCs via cryo-loop vitrification should be considered for genome banking of genetically modified mice and biomedical research.

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Mitochondrial characteristics in oocytes of the domestic cat (Felis catus) after in vitro maturation and vitrification

Cited by 10 publications

References 46 publications

Influence of interleukin 1 beta and tumour necrosis factor alpha on the in vitro growth, maturation and mitochondrial distribution of bovine oocytes from small antral follicles

Influence of interleukin 1 beta and tumour necrosis factor alpha on the in vitro growth, maturation and mitochondrial distribution of bovine oocytes from small antral follicles

Inhibition of Apoptotic Pathways Improves DNA Integrity but Not Developmental Competence of Domestic Cat Immature Vitrified Oocytes

Investigation of methods and factors influencing mouse oocyte quality, in vitro fertilization and embryo development

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